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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ARL2BP antibody [EPR15265-21] (ab188322)

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Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
  • Immunocytochemistry/ Immunofluorescence - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
  • Flow Cytometry (Intracellular) - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
  • Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
  • Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
  • Immunoprecipitation - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
  • Anti-ARL2BP antibody [EPR15265-21] (ab188322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15265-21] to ARL2BP
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-ARL2BP antibody [EPR15265-21]
    See all ARL2BP primary antibodies

  • Description

    Rabbit monoclonal [EPR15265-21] to ARL2BP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MCF7, Human fetal brain, HUVEC, A549, HepG2, Mouse brain, Mouse heart, Rat spleen, Raw264.7 and NIH/3T3 lysates. IHC-P: Human kidney and Rat liver tissues. ICC: A549 cells. Flow Cyt (intra): A549 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15265-21

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/200.
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
IP
1/40 - 1/60.
WB
1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
Notes
Flow Cyt (Intra)
1/200.
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
IP
1/40 - 1/60.
WB
1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

Target

  • Function

    Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. May play a role as an effector of ARL2.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the ARL2BP family.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Cytoplasm. Mitochondrion intermembrane space. Cytoplasm > cytoskeleton > centrosome. Nucleus. Cytoplasm > cytoskeleton > spindle. The complex formed with ARL2BP, ARL2 and SLC25A4 is expressed in mitochondria (By similarity). Detected in the midbody matrix. Not detected in the Golgi, nucleus and on the mitotic spindle. Centrosome-associated throughout the cell cycle. Not detected to interphase microtubules.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ADP ribosylation factor like 2 binding protein antibody
    • ADP-ribosylation factor-like protein 2-binding protein antibody
    • AR2BP_HUMAN antibody
    • Arf like 2 binding protein BART1 antibody
    • ARF-like 2-binding protein antibody
    • ARL2 binding protein antibody
    • Arl2bp antibody
    • ARL2BP protein antibody
    • BART antibody
    • BART1 antibody
    • Binder of ARF2 protein 1 antibody
    • Binder of Arl Two antibody
    • Binder of Arl2 antibody
    • Retinitis pigmentosa 66 (autosomal recessive) antibody
    • RP66 antibody
    see all

Images

  • Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    All lanes : Anti-ARL2BP antibody [EPR15265-21] (ab188322) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ARL2BP knockout HeLa cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 19 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab188322 observed at 20 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab188322 Anti-ARL2BP antibody [EPR15265-21] was shown to specifically react with ARL2BP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265269 (knockout cell lysate ab258312) was used. Wild-type and ARL2BP knockout samples were subjected to SDS-PAGE. ab188322 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (ab188322)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ARL2BP with ab188322 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Immunocytochemistry/ Immunofluorescence - Anti-ARL2BP antibody [EPR15265-21] (ab188322)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling ARL2BP with ab188322 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counterstained with Dapi (blue).

  • Flow Cytometry (Intracellular) - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Flow Cytometry (Intracellular) - Anti-ARL2BP antibody [EPR15265-21] (ab188322)

    Intracellular Flow Cytometry analysis of A549 (human lung carcinoma) cells labelling ARL2BP (red) with purified ab188322 at a dilution of 1/200. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody. 

     

     

  • Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    All lanes : Anti-ARL2BP antibody [EPR15265-21] (ab188322) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : HUVEC cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    All lanes : Anti-ARL2BP antibody [EPR15265-21] (ab188322) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Rat spleen tissue lysate
    Lane 4 : RAW 264.7 cell lysate
    Lane 5 : NIH/3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • Immunoprecipitation - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Immunoprecipitation - Anti-ARL2BP antibody [EPR15265-21] (ab188322)

    Western blot analysis of ARL2BP in HepG2 cell lysate immunoprecipitated with ab188322 at 1/50 dilution (Lane 1). Lane 2: PBS instead of HepG2 lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (ab188322)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ARL2BP with ab188322 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Anti-ARL2BP antibody [EPR15265-21] (ab188322)

References (0)

Publishing research using ab188322? Please let us know so that we can cite the reference in this datasheet.

ab188322 has not yet been referenced specifically in any publications.

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