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RecombinantRabMAb

Recombinant Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)

Submit a review Submit a question References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
  • Immunocytochemistry/ Immunofluorescence - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
  • Flow Cytometry (Intracellular) - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
  • Western blot - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
  • Western blot - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
  • Immunocytochemistry/ Immunofluorescence - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
  • Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10429] to Arp3 - C-terminal
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Arp3 antibody [EPR10429] - C-terminal
    See all Arp3 primary antibodies

  • Description

    Rabbit monoclonal [EPR10429] to Arp3 - C-terminal

  • Host species

    Rabbit

  • Specificity

    The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat, A549, Mouse spleen, Rat spleen, and Mouse brain lysates. IHC-P: human kidney, and mouse kidney tissues. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR10429

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  For unpurified please use at 1/40
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.

See IHC antigen retrieval protocols
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF
1/50 - 1/100.
Notes
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  For unpurified please use at 1/40
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.

See IHC antigen retrieval protocols
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF
1/50 - 1/100.

Target

  • Relevance

    The Arp2/3 complex involved in Golgi polerization in NIH3T3 cells. In a different model it has been shown that the Arp2/3 complex is necessary for neutrophil chemotaxis and phagocytosis.

  • Cellular localization

    Cytoplasmic

  • Database links

    • Alternative names

      • Actin like protein 3 antibody
      • Actin related protein 3 antibody
      • ACTR3 antibody
      • ARP3 actin related protein 3 homolog (yeast) antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Arp3 with purified ab181164 at 1:250 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunocytochemistry/ Immunofluorescence - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      Immunocytochemistry/ Immunofluorescence - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Arp3 with purified ab181164 at 1:50 dilution (5.06 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Flow Cytometry (Intracellular) - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      Flow Cytometry (Intracellular) - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)

      Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Arp3 with purified ab181164 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Arp3 with purified ab181164 at 1:250 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      Western blot - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      All lanes : Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse spleen lysates
      Lane 3 : Rat spleen lysates
      Lane 4 : Mouse brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 47 kDa
      Observed band size: 47 kDa

    • Western blot - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      Western blot - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      All lanes : Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164) at 1/10000 dilution (Unpurified)

      Lane 1 : HeLa lysate
      Lane 2 : Jurkat lysate
      Lane 3 : A549 lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 47 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      Immunocytochemistry/ Immunofluorescence - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)

      Immunofluorescence analysis of -20°C Acetone fixed HeLa cells labeling Arp3 with unpurified ab181164 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)

      Immunohistochemical analysis of paraffin embedded human kidney tissue labeling Arp3 with unpurified ab181164 at a 1/250 dilution. Prediluted HRP Rabbit IgG secondary was used. Counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
      Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)

    References (2)

    Publishing research using ab181164? Please let us know so that we can cite the reference in this datasheet.

    ab181164 has been referenced in 2 publications.

    • Li Y  et al. Dragon's Blood Regulates Rac1-WAVE2-Arp2/3 Signaling Pathway to Protect Rat Intestinal Epithelial Barrier Dysfunction Induced by Simulated Microgravity. Int J Mol Sci 22:N/A (2021). PubMed: 33800361
    • Wu D  et al. Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells. Oncotarget 8:88630-88644 (2017). WB ; Mouse . PubMed: 29179463

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