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RecombinantRabMAb

Recombinant Anti-ARPC2 antibody [EPR8533] (ab133315)

Reviews (3) Submit a question References (12)
Western blot - Anti-ARPC2 antibody [EPR8533] (ab133315)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [EPR8533] (ab133315)
  • Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody [EPR8533] (ab133315)
  • Flow Cytometry (Intracellular) - Anti-ARPC2 antibody [EPR8533] (ab133315)
  • Immunoprecipitation - Anti-ARPC2 antibody [EPR8533] (ab133315)
  • Western blot - Anti-ARPC2 antibody [EPR8533] (ab133315)
  • Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody [EPR8533] (ab133315)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [EPR8533] (ab133315)
  • OI-RD Scanning - Anti-ARPC2 antibody [EPR8533] (ab133315)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [EPR8533] (ab133315)
  • Anti-ARPC2 antibody [EPR8533] (ab133315)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8533] to ARPC2
  • Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 APC Carrier Free HRP PE

Overview

  • Product name

    Anti-ARPC2 antibody [EPR8533]
    See all ARPC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8533] to ARPC2

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ARPC2 aa 250-350. The exact sequence is proprietary.
    Database link: O15144

  • Positive control

    • IHC-P: Human kidney, tonsil and gastric carcinoma tissue; ICC/IF: HeLa cells; WB: HEK-293, Mouse heart, HT-1080, Jurkat, HeLa, Neuro-2a, and Rat heart lysates IP: HEK-293 lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 1.00 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8533

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/100 - 1/500.
Flow Cyt (Intra)
1/50.
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
IP
1/10 - 1/100.
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/250 - 1/500.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Notes
ICC/IF
1/100 - 1/500.
Flow Cyt (Intra)
1/50.
WB
1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
IP
1/10 - 1/100.
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/250 - 1/500.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Target

  • Function

    Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.

  • Sequence similarities

    Belongs to the ARPC2 family.

  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Actin related protein 2/3 complex subunit 2 34kDa antibody
    • Actin related protein 2/3 complex subunit 2 antibody
    • Actin-related protein 2/3 complex subunit 2 antibody
    • ARC 34 antibody
    • ARC34 antibody
    • Arp2/3 complex 34 kDa subunit antibody
    • ARP2/3 protein compex subunit 34 antibody
    • ARPC 2 antibody
    • Arpc2 antibody
    • ARPC2_HUMAN antibody
    • p34 Arc antibody
    • p34-ARC antibody
    • p34Arc antibody
    • PNAS 139 antibody
    • PNAS139 antibody
    • PRO 2446 antibody
    • PRO2446 antibody
    see all

Images

  • Western blot - Anti-ARPC2 antibody [EPR8533] (ab133315)
    Western blot - Anti-ARPC2 antibody [EPR8533] (ab133315)
    All lanes : Anti-ARPC2 antibody [EPR8533] (ab133315) at 1/1000 dilution (Purified)

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
    Lane 4 : Rat heart lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [EPR8533] (ab133315)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [EPR8533] (ab133315)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue sections labeling ARPC2 with purified ab133315 at 1/2000 dilution (0.25 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody [EPR8533] (ab133315)
    Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody [EPR8533] (ab133315)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ARPC2 with purified ab133315 at 1:100 dilution (5.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry (Intracellular) - Anti-ARPC2 antibody [EPR8533] (ab133315)
    Flow Cytometry (Intracellular) - Anti-ARPC2 antibody [EPR8533] (ab133315)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ARPC2 with purified ab133315 at 1/50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Immunoprecipitation - Anti-ARPC2 antibody [EPR8533] (ab133315)
    Immunoprecipitation - Anti-ARPC2 antibody [EPR8533] (ab133315)
    ab133315 (purified ) at 1/30 dilution (2 µg) immunoprecipitating ARPC2 in HEK-293 whole cell lysate.
    Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab133315 & HEK-293 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133315 in HEK-293 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • Western blot - Anti-ARPC2 antibody [EPR8533] (ab133315)
    Western blot - Anti-ARPC2 antibody [EPR8533] (ab133315)
    All lanes : Anti-ARPC2 antibody [EPR8533] (ab133315) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysates
    Lane 2 : 293T cell lysates
    Lane 3 : HT-1080 cell lysates
    Lane 4 : Jurkat cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 34 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody [EPR8533] (ab133315)
    Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody [EPR8533] (ab133315)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling ARPC2 with purified ab133315 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [EPR8533] (ab133315)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [EPR8533] (ab133315)

    Immunohistochemical analysis of paraffin embedded human kidney tissue labelled with ab133315 (unpurified) at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-ARPC2 antibody [EPR8533] (ab133315)
    OI-RD Scanning - Anti-ARPC2 antibody [EPR8533] (ab133315)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [EPR8533] (ab133315)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [EPR8533] (ab133315)

    Immunohistochemical analysis of paraffin embedded human tonsil tissue labelled with ab133315 (unpurified) at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-ARPC2 antibody [EPR8533] (ab133315)
    Anti-ARPC2 antibody [EPR8533] (ab133315)

References (12)

Publishing research using ab133315? Please let us know so that we can cite the reference in this datasheet.

ab133315 has been referenced in 12 publications.

  • Indra I  et al. Sensing Actin Dynamics through Adherens Junctions. Cell Rep 30:2820-2833.e3 (2020). PubMed: 32101754
  • Jongkamonwiwat N  et al. Noise Exposures Causing Hearing Loss Generate Proteotoxic Stress and Activate the Proteostasis Network. Cell Rep 33:108431 (2020). PubMed: 33238128
  • Ozawa M  et al. Adherens junction regulates cryptic lamellipodia formation for epithelial cell migration. J Cell Biol 219:N/A (2020). PubMed: 32886101
  • Jozic I  et al. Pharmacological and Genetic Inhibition of Caveolin-1 Promotes Epithelialization and Wound Closure. Mol Ther 27:1992-2004 (2019). PubMed: 31409528
  • Choi J  et al. Pimozide suppresses cancer cell migration and tumor metastasis through binding to ARPC2, a subunit of the Arp2/3 complex. Cancer Sci 110:3788-3801 (2019). PubMed: 31571309
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-ARPC2 antibody [EPR8533]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (DU145 Prostate cancer cell line)
Permeabilization
Yes - 0.1% Triton X-100
Specification
DU145 Prostate cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 12 2020

Western blot abreview for Anti-ARPC2 antibody [EPR8533]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (DU145 Prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
DU145 Prostate cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 04 2020

Western blot abreview for Anti-ARPC2 antibody [EPR8533]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
20 µg
Specification
HEK293
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 30 2012

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