Recombinant Anti-Artemin antibody [EPR9763] (ab178434)
![Immunoprecipitation - Anti-Artemin antibody [EPR9763] (ab178434)](https://www.abcam.com/ps/products/178/ab178434/Images/ab178434-7-anti-artemin-antibody-epr9763-immunoprecipitation-pc12-rat.jpg "Immunoprecipitation - Anti-Artemin antibody [EPR9763] (ab178434)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9763] to Artemin
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Artemin antibody [EPR9763]
See all Artemin primary antibodies -
Description
Rabbit monoclonal [EPR9763] to Artemin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Artemin aa 150 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q5T4W7 -
Positive control
- WB: T-47D, NIH/3T3 and PC-12 whole cell lysate. Mouse and rat brain lysate; PC-12 whole cell lysate. IP: PC-12 whole cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9763 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
|
|
| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
|
IP
1/10 - 1/100. |
Application notes
Is unsuitable for IHC-P.
Target
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Function
Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. -
Tissue specificity
Ubiquitous. Expressed at high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. Expressed at low levels in the brain. -
Sequence similarities
Belongs to the TGF-beta family. GDNF subfamily. -
Developmental stage
Expressed during embryogenesis. High level expression seen in fetal kidney and lung while a low level expression seen in the fetal brain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9048 Human
- Entrez Gene: 11876 Mouse
- Entrez Gene: 362572 Rat
- Omim: 603886 Human
- SwissProt: Q5T4W7 Human
- SwissProt: Q9Z0L2 Mouse
- SwissProt: Q6AYE8 Rat
- Unigene: 632404 Human
see all -
Alternative names
- Artemin antibody
- Artn antibody
- ARTN_HUMAN antibody
see all
Images
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Immunoprecipitation - Anti-Artemin antibody [EPR9763] (ab178434)Purified ab178434 at 1/30 dilution (2µg) immunoprecipitating Artemin in PC-12 whole cell lysate.
Lane 1 (input): PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate 10µg
Lane 2 (+): ab178434 + PC-12 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab178434 in PC-12 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDa -
![Western blot - Anti-Artemin antibody [EPR9763] (ab178434)](https://www.abcam.com/ps/products/178/ab178434/Images/ab178434-449364-anti-artemin-antibody-epr9763-westernblot-human-mouse-rat.jpg)
Western blot - Anti-Artemin antibody [EPR9763] (ab178434)All lanes : Anti-Artemin antibody [EPR9763] (ab178434) at 1/1000 dilution
Lane 1 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : Rat brain lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 23 kDa -
![Anti-Artemin antibody [EPR9763] (ab178434)](https://www.abcam.com/ps/products/178/ab178434/Images/ab178434-5-benefits-of-recombinant-antibodies.png)
Anti-Artemin antibody [EPR9763] (ab178434)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab178434 has been referenced in 2 publications.
- Song Z et al. Role of artemin in non-small cell lung cancer. Thorac Cancer 9:555-562 (2018). PubMed: 29575549
- Wang J et al. Artemin regulates CXCR4 expression to induce migration and invasion in pancreatic cancer cells through activation of NF-?B signaling. Exp Cell Res 365:12-23 (2018). Human . PubMed: 29453972