Anti-ASF1b antibody (ab70126)
Key features and details
- Rabbit polyclonal to ASF1b
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-ASF1b antibody
See all ASF1b primary antibodies -
Description
Rabbit polyclonal to ASF1b -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthesized peptide derived from internal of human ASF1B.
-
Positive control
- Extracts from K562 cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 22 kDa.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 22 kDa. |
Target
-
Function
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis. -
Tissue specificity
Highly expressed in testis and at lower levels in colon, small intestine and thymus. -
Sequence similarities
Belongs to the ASF1 family. -
Post-translational
modificationsPhosphorylated by TLK1 and TLK2. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 55723 Human
- Entrez Gene: 66929 Mouse
- Entrez Gene: 304648 Rat
- Omim: 609190 Human
- SwissProt: Q9NVP2 Human
- SwissProt: Q9DAP7 Mouse
- Unigene: 26516 Human
- Unigene: 29680 Mouse
-
Alternative names
- Anti silencing function 1B antibody
- Anti silencing function 1B histone chaperone antibody
- Anti-silencing function protein 1 homolog B antibody
see all
Images
-
Western blot - Anti-ASF1b antibody (ab70126)All lanes : Anti-ASF1b antibody (ab70126) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab70126 has not yet been referenced specifically in any publications.