Recombinant Anti-ASIC1 antibody [EPR25411-161] (ab300563)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR25411-161] to ASIC1
Suitable for: WB, IP, IHC-P
Reacts with: Mouse, Rat

Carrier Free

Product name

Anti-ASIC1 antibody [EPR25411-161]
See all ASIC1 primary antibodies
Description

Rabbit monoclonal [EPR25411-161] to ASIC1
Host species

Rabbit
Tested applications

Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra) or ICC/IF
Species reactivity

Reacts with: Mouse, Rat
Does not react with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Tissue lysates: Mouse cerebellum, brain and spinal cord; Rat brain and spinal cord. IHC-P: Mouse and rat dorsal root ganglion, HEK-293T transfected with a human ASIC1 expression vector. IP: Mouse cerebellum tissue lysate
General notes
ab300563 does not react in ICC and intracellular flow cytometry with mouse and rat species.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR25411-161
Isotype

IgG
Research areas
- Neuroscience
- Processes

Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab300563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Predicted molecular weight: 59 kDa.
IP		1/30.
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
WB 1/1000. Predicted molecular weight: 59 kDa.
IP 1/30.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt (Intra) or ICC/IF.

Function

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
Tissue specificity

Expressed in most or all neurons.
Sequence similarities

Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN2 subfamily.
Post-translational
modifications

Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.
Cellular localization

Cell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4.
Target information above from: UniProt accession P78348 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 11419 Mouse
- Entrez Gene: 79123 Rat
- SwissProt: Q6NXK8 Mouse
- SwissProt: P55926 Rat
- Unigene: 440107 Mouse
- Unigene: 37385 Rat
Alternative names
- ACCN2 antibody
- ACCN2_HUMAN antibody
- Acid sensing ion channel 1 antibody
- Acid sensing ion channel 1a protein antibody
- Acid sensing ion channel antibody
- Acid-sensing ion channel 1 antibody
- AI843610 antibody
- Amiloride sensitive cation channel 2 neuronal antibody
- Amiloride-sensitive cation channel 2 antibody
- ASIC antibody
- ASIC1 antibody
- ASIC1A antibody
- B530003N02Rik antibody
- BNaC2 antibody
- Brain sodium channel 2 antibody
- Cation channel amiloride sensitive neuronal 2 antibody
- hBNaC2 antibody
- neuronal antibody
see all

Western blot - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

All lanes : Anti-ASIC1 antibody [EPR25411-161] (ab300563) at 1/1000 dilution

Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse spinal cord tissue lysate
Lane 4 : Mouse heart tissue lysate
Lane 5 : Mouse kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : Goat anti-Rabbit IgG (HRP) with minimal cross reactivity with human IgG at 1/2000 dilution
Lanes 2-5 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 59 kDa
Observed band size: 70, 150 kDa why is the actual band size different from the predicted?

Exposure time: 48 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID: 19218436, PMID: 26252376).

Negative controls: mouse heart, kidney (PMID: 9037075).
Western blot - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

All lanes : Anti-ASIC1 antibody [EPR25411-161] (ab300563) at 1/1000 dilution

Lane 1 : Rat brain tissue lysate
Lane 2 : Rat spinal cord tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 59 kDa
Observed band size: 70, 150 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID: 19218436, PMID: 26252376).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded mouse dorsal root ganglion tissue labeling ASIC1 with ab300563 at 1/1000 dilution (0.498 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in mouse dorsal root ganglion neurons. The section was incubated with ab300563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue labeling ASIC1 with ab300563 at 1/1000 dilution (0.498 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in rat dorsal root ganglion neurons. The section was incubated with ab300563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded HEK-293T cells labeling ASIC1 with ab300563 at 1/5000 dilution (0.1 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on (A) HEK-293T transfected with a human ASIC1 expression vector, no staining on (B) HEK-293T cells transfected with a human ASIC2a expression vector and (C) HEK-293T transfected with an empty vector. The section was incubated with ab300563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling ASIC1 with ab300563 at 1/1000 dilution (0.498 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining in mouse cardiac muscle. The section was incubated with ab300563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling ASIC1 with ab300563 at 1/1000 dilution (0.498 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining in rat cardiac muscle. The section was incubated with ab300563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunoprecipitation - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

ASIC1 was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate with ab300563 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300563 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse cerebellum tissue lysate 10 μg

Lane 2: ab300563 IP in mouse cerebellum tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300563 in mouse cerebellum tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes
Anti-ASIC1 antibody [EPR25411-161] (AB300563)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab300563? Please let us know so that we can cite the reference in this datasheet.

ab300563 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (0)

Customer reviews and Q&As

Post-translational
modifications