Recombinant Anti-ASIC1 antibody [EPR25411-45] (ab284406)
![Western blot - Anti-ASIC1 antibody [EPR25411-45] (ab284406)](https://www.abcam.com/ps/products/284/ab284406/Images/ab284406-476157-anti-acidsensing-ion-channel-1-antibody-epr2541145-western-blot-lysate1.jpg "Western blot - Anti-ASIC1 antibody [EPR25411-45] (ab284406)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25411-45] to ASIC1
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-ASIC1 antibody [EPR25411-45]
See all ASIC1 primary antibodies -
Description
Rabbit monoclonal [EPR25411-45] to ASIC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF,IHC-Fr or IHC-P -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain, Mouse cerebellum and Rat brain, Rat cerebellum lysates. IP: Mouse cerebellum, Rat cerebellum cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25411-45 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab284406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 60 kDa.
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| IP |
1/30.
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| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 60 kDa. |
|
IP
1/30. |
Target
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Function
Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear. -
Tissue specificity
Expressed in most or all neurons. -
Sequence similarities
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN2 subfamily. -
Post-translational
modificationsPhosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel. -
Cellular localization
Cell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4. - Information by UniProt
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Database links
- Entrez Gene: 11419 Mouse
- Entrez Gene: 79123 Rat
- SwissProt: Q6NXK8 Mouse
- SwissProt: P55926 Rat
- Unigene: 440107 Mouse
- Unigene: 37385 Rat
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Alternative names
- ACCN2 antibody
- ACCN2_HUMAN antibody
- Acid sensing ion channel 1 antibody
see all
Images
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Western blot - Anti-ASIC1 antibody [EPR25411-45] (ab284406)All lanes : Anti-ASIC1 antibody [EPR25411-45] (ab284406) at 1/1000 dilution
Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 70, 150 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The observed MW are consistent with what has been described in the literature (PMID: 19218436, PMID: 26252376).
Exposure time: 3 minutes
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![Western blot - Anti-ASIC1 antibody [EPR25411-45] (ab284406)](https://www.abcam.com/ps/products/284/ab284406/Images/ab284406-476156-anti-acidsensing-ion-channel-1-antibody-epr2541145-western-blot-lysate.jpg)
Western blot - Anti-ASIC1 antibody [EPR25411-45] (ab284406)All lanes : Anti-ASIC1 antibody [EPR25411-45] (ab284406) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The observed MW is consistent with what has been described in the literature (PMID: 19218436, PMID: 26252376).
Negative control: heart, kidney (PMID: 9037075).
Exposure time: 15 seconds
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![Immunoprecipitation - Anti-ASIC1 antibody [EPR25411-45] (ab284406)](https://www.abcam.com/ps/products/284/ab284406/Images/ab284406-1-anti-acidsensing-ion-channel-1-antibody-epr2541145-immunoprecipitation-mouse-cerebellum-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-ASIC1 antibody [EPR25411-45] (ab284406)Acid-sensing ion channel 1 was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate 10 ug with ab284406 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab284406 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse cerebellum tissue lysate 10 ug
Lane 2: ab284406 IP in Mouse cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab284406 in mouse cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds
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![Immunoprecipitation - Anti-ASIC1 antibody [EPR25411-45] (ab284406)](https://www.abcam.com/ps/products/284/ab284406/Images/ab284406-2-anti-acidsensing-ion-channel-1-antibody-epr2541145-immunoprecipitation-rat-cerebellum-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-ASIC1 antibody [EPR25411-45] (ab284406)Acid-sensing ion channel 1 was immunoprecipitated from 0.35 mg Rat cerebellum tissue lysate 10 ug with ab284406 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab284406 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat cerebellum tissue lysate 10 ug
Lane 2: ab284406 IP in Rat cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab284406 in rat cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab284406 has not yet been referenced specifically in any publications.