Recombinant Anti-ASIC3 antibody [EPR26557-87] (ab305243)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26557-87] to ASIC3
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-ASIC3 antibody [EPR26557-87]
See all ASIC3 primary antibodies -
Description
Rabbit monoclonal [EPR26557-87] to ASIC3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Frmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat dorsal ganglion tissue lysate. IHC-Fr: Mouse and rat dorsal root ganglion (fresh).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26557-87 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab305243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 58 kDa.
Not suitable for mouse and human |
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IHC-Fr |
1/100.
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Notes |
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WB
1/1000. Predicted molecular weight: 58 kDa. Not suitable for mouse and human |
IHC-Fr
1/100. |
Target
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Function
Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. -
Tissue specificity
Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis. -
Sequence similarities
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN3 subfamily. -
Developmental stage
Expressed in fetal tissues, expression increases in lung and kidney adult tissues. -
Domain
The PDZ domain-binding motif is involved in interaction with LIN7A, GOPC and MAGI1. -
Post-translational
modificationsPhosphorylated by PKA. Phosphorylated by PKC. In vitro, PRKCABP/PICK-1 is necessary for PKC phosphorylation and activation of a ACCN3/ASIC3-ACCN1/ASIC2b channel, but does not activate a homomeric ACCN3 channel. -
Cellular localization
Cell membrane. Cytoplasm. Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells. - Information by UniProt
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Database links
- Entrez Gene: 171209 Mouse
- Entrez Gene: 286920 Rat
- SwissProt: Q6X1Y6 Mouse
- SwissProt: O35240 Rat
- Unigene: 299636 Mouse
- Unigene: 24225 Rat
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Alternative names
- ACCN 3 antibody
- Accn3 antibody
- ACCN3_HUMAN antibody
see all
Images
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All lanes : Anti-ASIC3 antibody [EPR26557-87] (ab305243) at 1/1000 dilution
Lane 1 : Rat dorsal ganglion tissue lysate
Lane 2 : Rat lung tissue lysate
Lane 3 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDaNegative controls: kidney, lung (PMID:11872753).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 26 seconds.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse dorsal root ganglion (fresh) tissue labeling Acid-sensing ion channel 3 with ab305243 at 1/100 dilution (4.76 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
Confocal image showing positive staining on mouse dorsal root ganglion.
The nuclear counterstain was DAPI (Blue). The section was incubated with ab305243 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse heart (fresh) tissue labeling Acid-sensing ion channel 3 with ab305243 at 1/100 dilution (4.76 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
Negative control: confocal image showing no staining on mouse heart (PMID: 9707631).
The nuclear counterstain was DAPI (Blue). The section was incubated with ab305243 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat dorsal root ganglion (fresh) tissue labeling Acid-sensing ion channel 3 with ab305243 at 1/100 dilution (4.76 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
Confocal image showing positive staining on rat dorsal root ganglion.
The nuclear counterstain was DAPI (Blue). The section was incubated with ab305243 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat heart (fresh) tissue labeling Acid-sensing ion channel 3 with ab305243 at 1/100 dilution (4.76 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
Negative control: confocal image showing no staining on rat heart (PMID: 9707631).
The nuclear counterstain was DAPI (Blue). The section was incubated with ab305243 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab305243 has not yet been referenced specifically in any publications.