For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/atad2-antibody-epr12730-ab176319.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Plasma Membrane ATPases
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ATAD2 antibody [EPR12730] (ab176319)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-ATAD2 antibody [EPR12730] (ab176319)
  • Flow Cytometry (Intracellular) - Anti-ATAD2 antibody [EPR12730] (ab176319)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATAD2 antibody [EPR12730] (ab176319)
  • Western blot - Anti-ATAD2 antibody [EPR12730] (ab176319)
  • Western blot - Anti-ATAD2 antibody [EPR12730] (ab176319)
  • Anti-ATAD2 antibody [EPR12730] (ab176319)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12730] to ATAD2
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free HRP

You may also be interested in

Protein
Product image
Recombinant Human ATAD2 protein (ab153302)
Primary
Product image
Anti-TSPAN1 antibody (ab254730)
Knockout
Product image
Human ATAD2 knockout HeLa cell line (ab264957)

View more associated products

Overview

  • Product name

    Anti-ATAD2 antibody [EPR12730]
    See all ATAD2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12730] to ATAD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ATAD2 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q6PL18

  • Positive control

    • WB: HeLa, MCF-7, T47-D and Saos-2 cell lysates. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12730
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Estrogen
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Bromodomain containing
    • Epigenetics and Nuclear Signaling
    • Bromodomains

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-ATAD2 antibody [EPR12730] (ab201353)
    • HRP Anti-ATAD2 antibody [EPR12730] (ab201743)
    • Anti-ATAD2 antibody [EPR12730] - BSA and Azide free (ab249904)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ATAD2 knockout HeLa cell line (ab264957)
  • KO cell lysates

    • Human ATAD2 knockout HeLa cell lysate (ab257359)
  • Positive Controls

    • T-47D whole cell lysate (ab14899)
    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human ATAD2 protein (ab153302)
  • Related Products

    • Recombinant Human ATAD2 protein (ab153302)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab176319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/60.
WB
1/1000 - 1/10000. Predicted molecular weight: 159 kDa.
ICC/IF
1/100 - 1/250.
Notes
Flow Cyt (Intra)
1/60.
WB
1/1000 - 1/10000. Predicted molecular weight: 159 kDa.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    May be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells.
  • Tissue specificity

    Highly expressed in estrogen receptor positive breast tumors and in osteosarcoma tumors.
  • Sequence similarities

    Belongs to the AAA ATPase family.
    Contains 1 bromo domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q6PL18 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29028 Human
    • Omim: 611941 Human
    • SwissProt: Q6PL18 Human
    • Unigene: 370834 Human
    • Alternative names

      • AAA nuclear coregulator cancer-associated protein antibody
      • AAA nuclear coregulator, cancer-associated antibody
      • ANCCA antibody
      • Atad2 antibody
      • ATAD2_HUMAN antibody
      • ATPase family AAA domain containing 2 antibody
      • ATPase family AAA domain containing protein 2 antibody
      • ATPase family AAA domain-containing protein 2 antibody
      • CT137 antibody
      • DKFZp667N1320 antibody
      • L16 antibody
      • MGC131938 antibody
      • MGC142216 antibody
      • MGC29843 antibody
      • MGC5254 antibody
      • PRO2000 antibody
      see all

    Images

    • Western blot - Anti-ATAD2 antibody [EPR12730] (ab176319)
      Western blot - Anti-ATAD2 antibody [EPR12730] (ab176319)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: ATAD2 knockout HAP1 whole cell lysate (20 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab176319 observed at 200 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab176319 was shown to specifically react with ATAD2 in wild type cells as signal was lost in ATAD2 knockout cells. Wild-type and ATAD2 knockout samples were subjected to SDS-PAGE. Ab176319 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

       

    • Flow Cytometry (Intracellular) - Anti-ATAD2 antibody [EPR12730] (ab176319)
      Flow Cytometry (Intracellular) - Anti-ATAD2 antibody [EPR12730] (ab176319)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ATAD2 (red) with ab176319 at a 1/60 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-ATAD2 antibody [EPR12730] (ab176319)
      Immunocytochemistry/ Immunofluorescence - Anti-ATAD2 antibody [EPR12730] (ab176319)

      Immunofluorescent staining of HeLa cells labeling ATAD2 using ab176319 at a 1/100 dilution.

    • Western blot - Anti-ATAD2 antibody [EPR12730] (ab176319)
      Western blot - Anti-ATAD2 antibody [EPR12730] (ab176319)
      All lanes : Anti-ATAD2 antibody [EPR12730] (ab176319) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : ATAD2 CRISPR/Cas9 edited HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 159 kDa
      Observed band size: 200 kDa why is the actual band size different from the predicted?



      Lanes 1- 2: Merged signal (red and green). Green - ab176319 observed at 200 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

       ab176319 was shown to react with ATAD2 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab264957 (CRISPR/Cas9 edited cell lysate ab257359) lane below 200kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and ATAD2 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab176319 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-ATAD2 antibody [EPR12730] (ab176319)
      Western blot - Anti-ATAD2 antibody [EPR12730] (ab176319)
      All lanes : Anti-ATAD2 antibody [EPR12730] (ab176319) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : MCF-7 lysate
      Lane 3 : T47-D lysate
      Lane 4 : Saos-2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 159 kDa

    • Anti-ATAD2 antibody [EPR12730] (ab176319)
      Anti-ATAD2 antibody [EPR12730] (ab176319)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab176319? Please let us know so that we can cite the reference in this datasheet.

    ab176319 has been referenced in 2 publications.

    • Cao LJ  et al. ATAD2 interacts with C/EBPß to promote esophageal squamous cell carcinoma metastasis via TGF-ß1/Smad3 signaling. J Exp Clin Cancer Res 40:109 (2021). PubMed: 33757572
    • Wu S  et al. Overexpression of microRNA-186 inhibits angiogenesis in retinoblastoma via the Hedgehog signaling pathway by targeting ATAD2. J Cell Physiol N/A:N/A (2019). PubMed: 30993715

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab176319.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.