Recombinant Anti-ATAD2 antibody [EPR12730] - BSA and Azide free (ab249904)
![Western blot - Anti-ATAD2 antibody [EPR12730] - BSA and Azide free (ab249904)](https://www.abcam.com/ps/products/249/ab249904/Images/ab249904-397587-recombinant-anti-calnexin-antibody-epr36332-western-blot-hela-edited-lysates.jpg "Western blot - Anti-ATAD2 antibody [EPR12730] - BSA and Azide free (ab249904)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12730] to ATAD2 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ATAD2 antibody [EPR12730] - BSA and Azide free
See all ATAD2 primary antibodies -
Description
Rabbit monoclonal [EPR12730] to ATAD2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra), WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell lysate.
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General notes
ab249904 is the carrier-free version of ab176319.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12730 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 159 kDa.
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| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 159 kDa. |
Target
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Function
May be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells. -
Tissue specificity
Highly expressed in estrogen receptor positive breast tumors and in osteosarcoma tumors. -
Sequence similarities
Belongs to the AAA ATPase family.
Contains 1 bromo domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 29028 Human
- Omim: 611941 Human
- SwissProt: Q6PL18 Human
- Unigene: 370834 Human
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Alternative names
- AAA nuclear coregulator cancer-associated protein antibody
- AAA nuclear coregulator, cancer-associated antibody
- ANCCA antibody
see all
Images
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Western blot - Anti-ATAD2 antibody [EPR12730] - BSA and Azide free (ab249904)All lanes : Anti-ATAD2 antibody [EPR12730] (ab176319) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ATAD2 CRISPR/Cas9 edited HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 159 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab176319).
Lanes 1- 2: Merged signal (red and green). Green - ab176319 observed at 200 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.
ab176319 was shown to react with ATAD2 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab264957 (CRISPR/Cas9 edited cell lysate ab257359) lane below 200kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and ATAD2 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab176319 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-ATAD2 antibody [EPR12730] - BSA and Azide free (ab249904)](https://www.abcam.com/ps/products/249/ab249904/Images/ab249904-2-benefits-of-recombinant-antibodies.png)
Anti-ATAD2 antibody [EPR12730] - BSA and Azide free (ab249904)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249904 has not yet been referenced specifically in any publications.
