Recombinant Anti-ATG4A antibody [EPR4122] - BSA and Azide free (ab247630)
![Western blot - Anti-ATG4A antibody [EPR4122] - BSA and Azide free (ab247630)](https://www.abcam.com/ps/products/247/ab247630/Images/ab247630-453276-anti-atg4a-antibody-epr4122-western-blot-hela-wt-atg4a-knockout-hela-hepg2-daudi-human.jpg "Western blot - Anti-ATG4A antibody [EPR4122] - BSA and Azide free (ab247630)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4122] to ATG4A - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ATG4A antibody [EPR4122] - BSA and Azide free
See all ATG4A primary antibodies -
Description
Rabbit monoclonal [EPR4122] to ATG4A - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal liver, HeLa, Daudi, HepG2, Jurkat and fetal brain lysates. Human brain tissue and Human skeletal muscle tissue.
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General notes
ab247630 is the carrier-free version of ab108322.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR4122 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval.
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Target
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Function
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. -
Tissue specificity
Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. -
Sequence similarities
Belongs to the peptidase C54 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 115201 Human
- Entrez Gene: 666468 Mouse
- Omim: 300663 Human
- SwissProt: Q8WYN0 Human
- SwissProt: Q8C9S8 Mouse
- Unigene: 8763 Human
- Unigene: 102230 Mouse
- Unigene: 393320 Mouse
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Alternative names
- AI627006 antibody
- APG4 autophagy 4 homolog A antibody
- Apg4a antibody
see all
Images
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Western blot - Anti-ATG4A antibody [EPR4122] - BSA and Azide free (ab247630)All lanes : Anti-ATG4A antibody [EPR4122] (ab108322) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ATG4A knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab108322).
Lanes 1 - 4: Merged signal (red and green). Green - ab108322 observed at 45 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab108322 was shown to react with ATG4A in wild-type HeLa cells in Western blot with loss of signal observed in ATG4A knockout cell line ab265738 (ATG4A knockout cell lysate ab257846). Wild-type HeLa and ATG4A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab108322 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] - BSA and Azide free (ab247630)](https://www.abcam.com/ps/products/247/ab247630/Images/ab247630-2-anti-atg4a-antibody-epr4122-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] - BSA and Azide free (ab247630)This data was developed using ab108322, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108322 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] - BSA and Azide free (ab247630)](https://www.abcam.com/ps/products/247/ab247630/Images/ab247630-1-anti-atg4a-antibody-epr4122-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] - BSA and Azide free (ab247630)This data was developed using ab108322, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue using ab108322 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Western blot - Anti-ATG4A antibody [EPR4122] - BSA and Azide free (ab247630)](https://www.abcam.com/ps/products/247/ab247630/Images/ab247630-405868-anti-atg4a-antibody-epr4122-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ATG4A antibody [EPR4122] - BSA and Azide free (ab247630)All lanes : Anti-ATG4A antibody [EPR4122] (ab108322) at 1/1000 dilution
Lane 1 : Fetal liver tissue lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDaThis data was developed using ab108322, the same antibody clone in a different buffer formulation.
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![Anti-ATG4A antibody [EPR4122] - BSA and Azide free (ab247630)](https://www.abcam.com/ps/products/247/ab247630/Images/ab247630-4-benefits-of-recombinant-antibodies.png)
Anti-ATG4A antibody [EPR4122] - BSA and Azide free (ab247630)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247630 has not yet been referenced specifically in any publications.
