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    products/primary-antibodies/atg9a-antibody-epr24502-ab108338.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ub-like Proteins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ATG9A antibody [EPR2450(2)] (ab108338)

  • Datasheet
  • SDS
Reviews (14) Submit a question References (53)

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Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
  • Flow Cytometry (Intracellular) - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
  • Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
  • Immunoprecipitation - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
  • Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
  • Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
  • Flow Cytometry (Intracellular) - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
  • Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
  • Anti-ATG9A antibody [EPR2450(2)] (ab108338)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2450(2)] to ATG9A
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
Product image
Alexa Fluor® 488 Anti-ATG9A antibody [EPR2450(2)] (ab206252)

View more associated products

Overview

  • Product name

    Anti-ATG9A antibody [EPR2450(2)]
    See all ATG9A primary antibodies
  • Description

    Rabbit monoclonal [EPR2450(2)] to ATG9A
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, 293T, A375, cell line lysates; Mouse brain and rat brain cell lysates IHC-P: Paraffin-embedded human colon tissue; Human thyroid carcinoma tissue. ICC/IF: HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2450(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ub-like Proteins
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cardiovascular
    • Heart
    • Autophagy
    • APG gene products
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • APG gene products
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Cancer
    • Cell Death
    • Autophagy
    • APG gene products
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-ATG9A antibody [EPR2450(2)] (ab206252)
    • Alexa Fluor® 647 Anti-ATG9A antibody [EPR2450(2)] (ab206253)
    • Alexa Fluor® 555 Anti-ATG9A antibody [EPR2450(2)] (ab211969)
    • Anti-ATG9A antibody [EPR2450(2)] - BSA and Azide free (ab223528)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.
WB (8)
1/1000. Predicted molecular weight: 94 kDa.
IP
1/10 - 1/100.
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use 1/100 - 1/250.

 

ICC/IF (5)
1/50 - 1/100.
Notes
Flow Cyt (Intra)
1/20.
WB
1/1000. Predicted molecular weight: 94 kDa.
IP
1/10 - 1/100.
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use 1/100 - 1/250.

 

ICC/IF
1/50 - 1/100.

Target

  • Function

    Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.
  • Sequence similarities

    Belongs to the ATG9 family.
  • Cellular localization

    Cytoplasmic vesicle, autophagosome membrane. Golgi apparatus, trans-Golgi network membrane. Late endosome membrane. Endoplasmic reticulum membrane. Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation-induced redistribution depends on ULK1, ATG13, as well as SH3GLB1.
  • Target information above from: UniProt accession Q7Z3C6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 79065 Human
    • Entrez Gene: 245860 Mouse
    • Entrez Gene: 363254 Rat
    • Omim: 612204 Human
    • SwissProt: Q7Z3C6 Human
    • SwissProt: Q68FE2 Mouse
    • SwissProt: Q5FWU3 Rat
    • Unigene: 323363 Human
    • Unigene: 238266 Mouse
    • Unigene: 491221 Mouse
    • Unigene: 35248 Rat
    see all
  • Alternative names

    • APG9 autophagy 9-like 1 antibody
    • APG9 like 1 antibody
    • APG9-like 1 antibody
    • APG9L1 antibody
    • ATG9 antibody
    • ATG9 autophagy related 9 homolog A antibody
    • ATG9 autophagy related 9 homolog A (S. cerevisiae) antibody
    • ATG9A antibody
    • ATG9A_HUMAN antibody
    • Autophagy 9-like 1 protein antibody
    • Autophagy related 9A antibody
    • Autophagy related protein 9A antibody
    • Autophagy-related protein 9A antibody
    • mATG9 antibody
    • MGD3208 antibody
    • OTTHUMP00000206046 antibody
    • OTTHUMP00000206048 antibody
    • OTTHUMP00000206049 antibody
    • OTTHUMP00000206062 antibody
    see all

Images

  • Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: ATG9A knockout HAP1 cell lysate (20 µg)
    Lane 3: HepG2 cell lysate (20 µg)
    Lane 4: A375 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab108338 observed at 100 and 130 kDa. Red - loading control, ab8245, observed at 37 kDa.

    Unpurified ab108338 was shown to specifically react with ATG9A when ATG9A knockout samples were used. Wild-type and ATG9A knockout samples were subjected to SDS-PAGE. ab108338 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody [EPR2450(2)] (ab108338)De Pace, R. et al PLoS Genet. 2018 Apr 26;14(4):e1007363. doi: 10.1371/journal.pgen.1007363. eCollection 2018 Apr Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

    Accumulation of ATG9A at the TGN of AP-4 μ4 mutant patient fibroblasts.

    Skin fibroblasts were from one control individual and two patients homozygous for mutations in the AP4M1 gene encoding AP-4 μ4.

    Co-immunostaining for endogenous ATG9A (green) and AP-4 ε (red) (B) or GM130 of the fibroblasts.

    ATG9A is detected using ab108338.

     

    (From Figure 4B of De Pace et al)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG9A antibody [EPR2450(2)] (ab108338)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling ATG9A with Purified ab108338 at 1:50 dilution (4.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Flow Cytometry (Intracellular) - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Flow Cytometry (Intracellular) - Anti-ATG9A antibody [EPR2450(2)] (ab108338)

    Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ATG9A with purified ab108338 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 100% Methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody [EPR2450(2)] (ab108338)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ATG9A with Purified ab108338 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    All lanes : Anti-ATG9A antibody [EPR2450(2)] (ab108338) at 1/10000 dilution (purified)

    Lane 1 : 293 (Human embryonic kidney epithelial cell) whole cell lysate prepared in non-boiled method
    Lane 2 : 293 (Human embryonic kidney epithelial cell) whole cell lysate prepared in boiled method
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared in non-boiled method
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared in boiled method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 94 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    We suggest not to boil the sample after lysis.
    Blocking and diluting buffer: 5% NFDM/TBST

    Exposure time:  
    Left image: 5 seconds
    Right image: 2 seconds

     

  • Immunoprecipitation - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Immunoprecipitation - Anti-ATG9A antibody [EPR2450(2)] (ab108338)

    ab108338 (purified) at 1:20 dilution (2μg) immunoprecipitating ATG9A in HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate.

    Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10μg
    Lane 2 (+): ab108338 & HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108338 in HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution. No band in input lane is due to the boiled lysates
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Anti-ATG9A antibody [EPR2450(2)] (ab108338) at 1/2000 dilution (purified) + Mouse spinal cord lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 94 kDa



    Blocking and diluting buffer: 5% NFDM/TBST. 

    The lysates are boiled.

  • Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Anti-ATG9A antibody [EPR2450(2)] (ab108338) at 1/2000 dilution (purified) + Rat brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 94 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

    The lysates are boiled.

  • Flow Cytometry (Intracellular) - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Flow Cytometry (Intracellular) - Anti-ATG9A antibody [EPR2450(2)] (ab108338)

    Unpurified ab108338 staining ATG9Ain the human cell line HepG2 (human hepatocellular carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/40. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody. 

    Isoytype control: Rabbit monoclonal IgG (Black) 

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) 

     

     

  • Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Western blot - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    All lanes : Anti-ATG9A antibody [EPR2450(2)] (ab108338) at 1/1000 dilution (unpurified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A375 cell lysate
    Lane 4 : Mouse brain cell lysate
    Lane 5 : Rat brain cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 94 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG9A antibody [EPR2450(2)] (ab108338)

    Unpurified ab108338, at 1/100, staining ATG9A in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody [EPR2450(2)] (ab108338)

    Unpurified ab108338 at 1/50 dilution, staining ATG9A in HepG2 (Human hepatocellular carcinoma epithelial cell) cells by Immunofluorescence.

  • Anti-ATG9A antibody [EPR2450(2)] (ab108338)
    Anti-ATG9A antibody [EPR2450(2)] (ab108338)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (53)

Publishing research using ab108338? Please let us know so that we can cite the reference in this datasheet.

ab108338 has been referenced in 53 publications.

  • Rigby MJ  et al. Endoplasmic reticulum acetyltransferases Atase1 and Atase2 differentially regulate reticulophagy, macroautophagy and cellular acetyl-CoA metabolism. Commun Biol 4:454 (2021). PubMed: 33846551
  • Besemer AS  et al. Receptor-mediated endocytosis 8 (RME-8)/DNAJC13 is a novel positive modulator of autophagy and stabilizes cellular protein homeostasis. Cell Mol Life Sci 78:645-660 (2021). PubMed: 32322926
  • Xu Z  et al. MicroRNA-383 promotes reactive oxygen species-induced autophagy via downregulating peroxiredoxin 3 in human glioma U87 cells. Exp Ther Med 21:439 (2021). PubMed: 33747176
  • Zou J  et al. Electroacupuncture Inhibits Autophagy of Neuron Cells in Postherpetic Neuralgia by Increasing the Expression of miR-223-3p. Biomed Res Int 2021:6637693 (2021). PubMed: 33860046
  • Zellner S  et al. Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells. STAR Protoc 2:100506 (2021). PubMed: 33997820
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-10 of 14 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-ATG9A antibody [EPR2450(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (fibroblast)
Permeabilization
No
Specification
fibroblast
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 16 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-ATG9A antibody [EPR2450(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Fibroblast cells)
Permeabilization
No
Specification
Fibroblast cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 05 2021

Western blot abreview for Anti-ATG9A antibody [EPR2450(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (kidney)
Gel Running Conditions
Reduced Denaturing (4-12% bis-tris gel, MES buffer)
Loading amount
30 µg
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 02 2020

Western blot abreview for Anti-ATG9A antibody [EPR2450(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Dopaminergic Neurons)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Dopaminergic Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 19 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-ATG9A antibody [EPR2450(2)]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100
Specification
HeLa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Jerome Cattin

Verified customer

Submitted May 06 2019

Western blot abreview for Anti-ATG9A antibody [EPR2450(2)]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse hippocampal lysate)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Mouse hippocampal lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Davor Ivankovic

Verified customer

Submitted Jul 16 2018

Western blot abreview for Anti-ATG9A antibody [EPR2450(2)]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Cow Cell lysate - whole cell (Lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Apr 05 2017

Western blot abreview for Anti-ATG9A antibody [EPR2450(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuroblastoma)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
neuroblastoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Apr 05 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-ATG9A antibody [EPR2450(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney)
Permeabilization
Yes - 0.2% triton x100
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Mar 24 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-ATG9A antibody [EPR2450(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (kidney)
Permeabilization
Yes - 0.2% triton x100
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 24 2017

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