Recombinant Anti-ATOX1 antibody [EPR10352] (ab154179)
![Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)](https://www.abcam.com/ps/products/154/ab154179/Images/ab154179-410349-anti-atox1-antibody-epr10352-western-blot-human-knockout-lysate.jpg "Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10352] to ATOX1
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ATOX1 antibody [EPR10352]
See all ATOX1 primary antibodies -
Description
Rabbit monoclonal [EPR10352] to ATOX1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, HeLa and 293T cell lysates. IHC-P: Human prostatic hyperplasia tissue IP: HeLa.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10352 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 7 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
1/100 - 1/500.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 7 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
1/100 - 1/500. |
Target
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Function
Could bind and deliver cytosolic copper to the copper ATPase proteins. May be important in cellular antioxidant defense. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the ATX1 family.
Contains 1 HMA domain. - Information by UniProt
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Database links
- Entrez Gene: 475 Human
- Omim: 602270 Human
- SwissProt: O00244 Human
- Unigene: 125213 Human
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Alternative names
- ATOX1 antibody
- ATOX1_HUMAN antibody
- ATX1 antibody
see all
Images
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Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ATOX1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 7 kDa
Observed band size: 7 kDaLanes 1-4: Merged signal (red and green). Green - ab154179 observed at 7 kDa. Red - loading control ab8245 observed at 36 kDa.
ab154179 Anti-ATOX1 antibody [EPR10352] was shown to specifically react with ATOX1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266651 (knockout cell lysate ab257849) was used. Wild-type and ATOX1 knockout samples were subjected to SDS-PAGE. ab154179 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATOX1 antibody [EPR10352] (ab154179)](https://www.abcam.com/ps/products/154/ab154179/Images/ab154179-160112-anti-atox1-antibody-epr10352-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATOX1 antibody [EPR10352] (ab154179)Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling ATOX1 with ab154179 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)](https://www.abcam.com/ps/products/154/ab154179/Images/ab154179-414655-anti-atox1-antibody-epr10352-western-blot-hek293-wt-hek293t-atox1-knockout.jpg)
Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : ATOX1 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 7 kDa
Observed band size: 7 kDaLanes 1 - 2: Merged signal (red and green). Green - ab154179 observed at 7 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab154179 was shown to react with ATOX1 in HEK-293 wild-type cells in western blot with loss of signal observed in ATOX1 knockout sample. HEK-293 wild-type and ATOX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab154179 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)](https://www.abcam.com/ps/products/154/ab154179/Images/ab154179-160113-anti-atox1-antibody-epr10352-western-blot.jpg)
Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 7 kDa -
![Immunoprecipitation - Anti-ATOX1 antibody [EPR10352] (ab154179)](https://www.abcam.com/ps/products/154/ab154179/Images/ab154179-7-anti-atox1-antibody-epr10352-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-ATOX1 antibody [EPR10352] (ab154179)ATOX1 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with 154179 at 1/20 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab154179 IP in HeLa whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab154179 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-ATOX1 antibody [EPR10352] (ab154179)](https://www.abcam.com/ps/products/154/ab154179/Images/ab154179-5-benefits-of-recombinant-antibodies.png)
Anti-ATOX1 antibody [EPR10352] (ab154179)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab154179 has been referenced in 5 publications.
- Alvarez-Rodriguez M et al. mRNA expression of oxidative-reductive proteins in boars with documented different fertility can identify relevant prognostic biomarkers. Res Vet Sci 141:195-202 (2021). PubMed: 34763256
- Wen MH et al. Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1. Stem Cell Res 41:101631 (2019). PubMed: 31704540
- Nie Y et al. Lactobacillus frumenti improves antioxidant capacity via nitric oxide synthase 1 in intestinal epithelial cells. FASEB J 33:10705-10716 (2019). PubMed: 31262191
- Yee EMH et al. Dextran-Catechin inhibits angiogenesis by disrupting copper homeostasis in endothelial cells. Sci Rep 7:7638 (2017). PubMed: 28794411
- Blockhuys S & Wittung-Stafshede P Copper chaperone Atox1 plays role in breast cancer cell migration. Biochem Biophys Res Commun 483:301-304 (2017). WB ; Human . PubMed: 28027931