Recombinant Anti-ATP1A3 antibody [EPR14138] (ab182571)
![Western blot - Anti-ATP1A3 antibody [EPR14138] (ab182571)](https://www.abcam.com/ps/products/182/ab182571/Images/ab182571-506531-anti-atp1a3-antibody-epr14138-western-blot-brain-lysate-mouse-rat.jpg "Western blot - Anti-ATP1A3 antibody [EPR14138] (ab182571)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14138] to ATP1A3
- Suitable for: IP, WB, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-ATP1A3 antibody [EPR14138]
See all ATP1A3 primary antibodies -
Description
Rabbit monoclonal [EPR14138] to ATP1A3 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and Rat brain lysates,unboiled. ICC/IF: Mouse primary neuron cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14138 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/20 - 1/40.
|
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| WB |
1/1000. Detects a band of approximately 100-150 kDa (predicted molecular weight: 111 kDa).
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| ICC/IF |
1/50.
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| Notes |
|---|
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IP
1/20 - 1/40. |
|
WB
1/1000. Detects a band of approximately 100-150 kDa (predicted molecular weight: 111 kDa). |
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ICC/IF
1/50. |
Target
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Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
Involvement in disease
Dystonia 12
Alternating hemiplegia of childhood 2
Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 478 Human
- Entrez Gene: 232975 Mouse
- Entrez Gene: 24213 Rat
- Omim: 182350 Human
- SwissProt: P13637 Human
- SwissProt: Q6PIC6 Mouse
- SwissProt: P06687 Rat
- Unigene: 515427 Human
see all -
Alternative names
- AHC2 antibody
- Alpha(III) antibody
- AT1A3_HUMAN antibody
see all
Images
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Western blot - Anti-ATP1A3 antibody [EPR14138] (ab182571)All lanes : Anti-ATP1A3 antibody [EPR14138] (ab182571) at 1/1000 dilution
Lane 1 : Mouse brain lysate, unboiled
Lane 2 : Mouse brain lysate, boiled
Lane 3 : Rat brain lysate, unboiled
Lane 4 : Rat brain lysate, boiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 111 kDa
Observed band size: 102 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration:5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] (ab182571)](https://www.abcam.com/ps/products/182/ab182571/Images/ab182571-3-anti-atp1a3-antibody-epr14138-immunocytochemistry-neuron-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] (ab182571)Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling ATP1A3 with purified ab182571 at 1/50 (5.2 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. -
![Anti-ATP1A3 antibody [EPR14138] (ab182571)](https://www.abcam.com/ps/products/182/ab182571/Images/ab182571-4-benefits-of-recombinant-antibodies.png)
Anti-ATP1A3 antibody [EPR14138] (ab182571)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab182571 has been referenced in 1 publication.
- Fernández ÁF et al. Interaction between the autophagy protein Beclin 1 and Na+,K+-ATPase during starvation, exercise, and ischemia. JCI Insight 5:N/A (2020). PubMed: 31941841