Anti-ATP5A antibody [7H10BD4F9] (ab110273)
![Western blot - Anti-ATP5A antibody [7H10BD4F9] (ab110273)](https://www.abcam.com/ps/products/110/ab110273/Images/ab110273-123468-anti-atp5a-antibody-7h10bd4f9-western-blot.gif "Western blot - Anti-ATP5A antibody [7H10BD4F9] (ab110273)")
Key features and details
- Mouse monoclonal [7H10BD4F9] to ATP5A
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
Overview
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Product name
Anti-ATP5A antibody [7H10BD4F9]
See all ATP5A primary antibodies -
Description
Mouse monoclonal [7H10BD4F9] to ATP5A -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Full length protein corresponding to Cow ATP5A.
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Positive control
- Isolated mitochondria from Human, Rat, Bovine and Mouse Heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HL-60 cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110273 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
7H10BD4F9 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (6) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
|
| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
|
| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. -
Tissue specificity
Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord. -
Sequence similarities
Belongs to the ATPase alpha/beta chains family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Mitochondrion inner membrane. Peripheral membrane protein. - Information by UniProt
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Database links
- Entrez Gene: 282578 Cow
- Entrez Gene: 498 Human
- Entrez Gene: 11946 Mouse
- Entrez Gene: 65262 Rat
- Omim: 164360 Human
- SwissProt: P19483 Cow
- SwissProt: P25705 Human
- SwissProt: Q03265 Mouse
see all -
Alternative names
- ATP synthase alpha chain antibody
- ATP synthase alpha chain, mitochondrial antibody
- ATP synthase subunit alpha antibody
see all
Images
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Western blot - Anti-ATP5A antibody [7H10BD4F9] (ab110273)All lanes : Anti-ATP5A antibody [7H10BD4F9] (ab110273) at 1 µg/ml
Lane 1 : Human heart mitochondria at 5 µg
Lane 2 : Bovine heart mitochondria at 1 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Lane 5 : HepG2 mitochondria at 20 µg
Predicted band size: 60 kDa -
![Flow Cytometry - Anti-ATP5A antibody [7H10BD4F9] (ab110273)](https://www.abcam.com/ps/products/110/ab110273/Images/ab110273-123467-anti-atp5a-antibody-7h10bd4f9-flow-cytometry.jpg)
Flow Cytometry - Anti-ATP5A antibody [7H10BD4F9] (ab110273)HL-60 cells were stained with 1 µg/mL ab110273 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry. -
![Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [7H10BD4F9] (ab110273)](https://www.abcam.com/ps/products/110/ab110273/Images/ab110273-123466-anti-atp5a-antibody-7h10bd4f9-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [7H10BD4F9] (ab110273)Mitochondrial localization of ATP5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110273 (2 µg/ml) followed by an Alexa Fluor® 594-conjugated-goat-anti-mouse IgG2b isotype specific secondary antibody (2 µg/ml).
Datasheets and documents
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SDS download
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Datasheet download
References (71)
ab110273 has been referenced in 71 publications.
- Gómez-Caudillo L et al. Principal component analysis on LC-MS/MS and 2DE-MALDI-TOF in glioblastoma cell lines reveals that mitochondria act as organelle sensors of the metabolic state in glioblastoma. Oncol Rep 44:661-673 (2020). PubMed: 32468038
- Kalpage HA et al. Brain-Specific Serine-47 Modification of Cytochrome c Regulates Cytochrome c Oxidase Activity Attenuating ROS Production and Cell Death: Implications for Ischemia/Reperfusion Injury and Akt Signaling. Cells 9:N/A (2020). PubMed: 32781572
- Sun D et al. Mitochondrial DNA Haplogroup M7 Confers Disability in a Chinese Aging Population. Front Genet 11:577795 (2020). PubMed: 33193696
- Rodríguez LR et al. Oxidative stress modulates rearrangement of endoplasmic reticulum-mitochondria contacts and calcium dysregulation in a Friedreich's ataxia model. Redox Biol 37:101762 (2020). PubMed: 33128998
- Zhang L et al. Voltage-Dependent Anion Channels Influence Cytotoxicity of ME-344, a Therapeutic Isoflavone. J Pharmacol Exp Ther 374:308-318 (2020). PubMed: 32546528