Anti-ATP5C1 antibody [2A1AA11] (ab119686)
![Western blot - Anti-ATP5C1 antibody [2A1AA11] (ab119686)](https://www.abcam.com/ps/products/119/ab119686/Images/ab119686-132838-anti-atpg-antibody-2a1aa11-western-blot.jpg "Western blot - Anti-ATP5C1 antibody [2A1AA11] (ab119686)")
Key features and details
- Mouse monoclonal [2A1AA11] to ATP5C1
- Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
Related conjugates and formulations
Overview
-
Product name
Anti-ATP5C1 antibody [2A1AA11]
See all ATP5C1 primary antibodies -
Description
Mouse monoclonal [2A1AA11] to ATP5C1 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
Clonality
Monoclonal -
Clone number
2A1AA11 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.
|
|
| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
|
| IHC-P |
Use a concentration of 5 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
IHC-P
Use a concentration of 5 µg/ml. |
Target
-
Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. -
Tissue specificity
Isoform Heart is expressed specifically in the heart and skeletal muscle, which require rapid energy supply. Isoform Liver is expressed in the brain, liver and kidney. Isoform Heart and Isoform Liver are expressed in the skin, intestine, stomach and aorta. -
Sequence similarities
Belongs to the ATPase gamma chain family. -
Cellular localization
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
-
Database links
- Entrez Gene: 327668 Cow
- Entrez Gene: 509 Human
- Entrez Gene: 11949 Mouse
- Entrez Gene: 116550 Rat
- Omim: 108729 Human
- SwissProt: P05631 Cow
- SwissProt: P36542 Human
- SwissProt: Q91VR2 Mouse
see all -
Alternative names
- ATP synthase gamma chain, mitochondrial antibody
- ATP synthase subunit gamma antibody
- ATP synthase subunit gamma, mitochondrial antibody
see all
Images
-
Western blot - Anti-ATP5C1 antibody [2A1AA11] (ab119686)All lanes : Anti-ATP5C1 antibody [2A1AA11] (ab119686) at 1 µg/ml
Lane 1 : human heart homogenate lysate at 15 µg
Lane 2 : human HepG2 cell lysate at 15 µg
Lane 3 : human liver mitochondria lysate at 7.5 µg
Lane 4 : rat liver mitochondria lysate at 7.5 µg
Lane 5 : mouse liver mitochondria lysate at 7.5 µg
Lane 6 : bovine heart mitochondria lysate at 7.5 µg
Secondary
All lanes : Goat anti-mouse HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-ATP5C1 antibody [2A1AA11] (ab119686)](https://www.abcam.com/ps/products/119/ab119686/Images/ab119686-132837-anti-atpg-antibody-2a1aa11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ATP5C1 antibody [2A1AA11] (ab119686)Immunocytochemistry using ab119686 stained HDFn cells (human). The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) with antigen retrieval. The cells were then incubated with the antibody (ab119686, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria. -
![Flow Cytometry - Anti-ATP5C1 antibody [2A1AA11] (ab119686)](https://www.abcam.com/ps/products/119/ab119686/Images/ab119686-132836-anti-atpg-antibody-2a1aa11-flow-cytometry.jpg)
Flow Cytometry - Anti-ATP5C1 antibody [2A1AA11] (ab119686)Flow cytometry. Hela cells were stained with 1µg/mL anti-ATP5C1 antibody (ab119686) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5C1 antibody [2A1AA11] (ab119686)](https://www.abcam.com/ps/products/119/ab119686/Images/ab119686-132835-anti-atpg-antibody-2a1aa11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5C1 antibody [2A1AA11] (ab119686)IHC image of ATP5C1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119686, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
-
Datasheet download
References (7)
ab119686 has been referenced in 7 publications.
- Li K et al. Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNAThr Gene. J Diabetes Res 2020:2057187 (2020). PubMed: 32083134
- Wüst S et al. Metabolic Maturation during Muscle Stem Cell Differentiation Is Achieved by miR-1/133a-Mediated Inhibition of the Dlk1-Dio3 Mega Gene Cluster. Cell Metab 27:1026-1039.e6 (2018). PubMed: 29606596
- Krus U et al. Pyruvate dehydrogenase kinase 1 controls mitochondrial metabolism and insulin secretion in INS-1 832/13 clonal beta-cells. Biochem J 429:205-13 (2010). PubMed: 20415663
- Fogal V et al. Mitochondrial p32 protein is a critical regulator of tumor metabolism via maintenance of oxidative phosphorylation. Mol Cell Biol 30:1303-18 (2010). PubMed: 20100866
- Stenson BM et al. Activation of liver X receptor regulates substrate oxidation in white adipocytes. Endocrinology 150:4104-13 (2009). PubMed: 19556420
- Sharma P et al. Role of pyruvate dehydrogenase complex in traumatic brain injury and Measurement of pyruvate dehydrogenase enzyme by dipstick test. J Emerg Trauma Shock 2:67-72 (2009). PubMed: 19561963
- Ristorcelli E et al. Human tumor nanoparticles induce apoptosis of pancreatic cancer cells. FASEB J 22:3358-69 (2008). PubMed: 18511551
