Anti-ATP5D antibody [4D11] (ab174438)
![Western blot - Anti-ATP5D antibody [4D11] (ab174438)](https://www.abcam.com/ps/products/174/ab174438/Images/ab174438-199865-ab1744385.jpg "Western blot - Anti-ATP5D antibody [4D11] (ab174438)")
Key features and details
- Mouse monoclonal [4D11] to ATP5D
- Suitable for: WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-ATP5D antibody [4D11]
See all ATP5D primary antibodies -
Description
Mouse monoclonal [4D11] to ATP5D -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- Rat and mouse heart and liver homogenates, rat H4IIE lysate and mouse MEF cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.9% HEPES buffered saline -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
4D11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174438 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
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| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. |
Target
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Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. -
Sequence similarities
Belongs to the ATPase epsilon chain family. -
Cellular localization
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 338081 Cow
- Entrez Gene: 513 Human
- Entrez Gene: 66043 Mouse
- Entrez Gene: 245965 Rat
- Omim: 603150 Human
- SwissProt: P05630 Cow
- SwissProt: P30049 Human
- SwissProt: Q9D3D9 Mouse
see all -
Alternative names
- ATP synthase subunit delta, mitochondrial antibody
- ATP synthase subunit delta, mitochondrial antibody
- ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit antibody
see all
Images
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Western blot - Anti-ATP5D antibody [4D11] (ab174438)All lanes : Anti-ATP5D antibody [4D11] (ab174438) at 1 µg/ml (5% milk PBST)
Lane 1 : Human Heart Homogenate at 10 µg
Lane 2 : Human Liver Homogenate at 10 µg
Lane 3 : Rat Liver Homogenate at 10 µg
Lane 4 : Mouse Liver Homogenate at 10 µg
Lane 5 : Bovine Heart Homogenate at 10 µg
Lane 6 : Human HepG2 cell lysate at 20 µg
Lane 7 : Rat H4IIE cell lysate at 20 µg
Lane 8 : Mouse MEF cell lysate at 20 µg
Secondary
All lanes : Goat anti Mouse IR700 in 5% milk/PBST at 1/5000 dilution
Predicted band size: 17 kDa
Datasheets and documents
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Datasheet download
References (0)
ab174438 has not yet been referenced specifically in any publications.
