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Anti-ATP5F1 antibody [9D1BC4] (ab117991)

Submit a review Q&A (1)References (7)
Western blot - Anti-ATP5F1 antibody [9D1BC4] (ab117991)
  • Flow Cytometry - Anti-ATP5F1 antibody [9D1BC4] (ab117991)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5F1 antibody [9D1BC4] (ab117991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5F1 antibody [9D1BC4] (ab117991)

Key features and details

  • Mouse monoclonal [9D1BC4] to ATP5F1
  • Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-ATP5F1 antibody [9D1BC4]
    See all ATP5F1 primary antibodies

  • Description

    Mouse monoclonal [9D1BC4] to ATP5F1

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • Human normal colon FFPE tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab117991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 24.6 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 1 µg/ml.
Notes
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 24.6 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

  • Sequence similarities

    Belongs to the eukaryotic ATPase B chain family.

  • Cellular localization

    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AT5F1_HUMAN antibody
    • ATP synthase B chain mitochondrial antibody
    • ATP synthase subunit b antibody
    • ATP synthase subunit b mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 antibody
    • ATP5F1 antibody
    • ATPase subunit b antibody
    • Cell proliferation inducing protein 47 antibody
    • H+ ATP synthase subunit b antibody
    • MGC24431 antibody
    • mitochondrial antibody
    • PIG47 antibody
    see all

Images

  • Western blot - Anti-ATP5F1 antibody [9D1BC4] (ab117991)
    Western blot - Anti-ATP5F1 antibody [9D1BC4] (ab117991)
    All lanes : Anti-ATP5F1 antibody [9D1BC4] (ab117991) at 1 µg/ml

    Lane 1 : Human heart homogenate at 15 µg
    Lane 2 : HepG2 lysate at 15 µg
    Lane 3 : Human liver mitochondria at 7.5 µg
    Lane 4 : Bovine heart mitochondria at 7.5 µg
    Lane 5 : Rat liver mitochondria at 7.5 µg
    Lane 6 : Mouse liver mitochondria at 7.5 µg

    Predicted band size: 24.6 kDa



    Note - Extra bands seen in lane 6 are secondary antibody mouse-on-mouse effects and not related to the primary antibody.
  • Flow Cytometry - Anti-ATP5F1 antibody [9D1BC4] (ab117991)
    Flow Cytometry - Anti-ATP5F1 antibody [9D1BC4] (ab117991)
    ab117991 at 1 ug/ml with Hela cells in flow cytometry.
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5F1 antibody [9D1BC4] (ab117991)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP5F1 antibody [9D1BC4] (ab117991)
    Immunocytochemistry image of ab117991 (MS972) stained NIH3T3 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) with urea/heat antigen retrieval method. The cells were incubated with ab117991 at 1 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates to the mitochondria. The four cells in the upper portion of the image show mitochondria in the elongated, reticular, arrangement, the three cells in the lower portion of the image show a punctuate mitochondrial organization and may be dividing/have recently divided.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5F1 antibody [9D1BC4] (ab117991)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5F1 antibody [9D1BC4] (ab117991)

    IHC image of ATP5F1 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117991, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References (7)

Publishing research using ab117991? Please let us know so that we can cite the reference in this datasheet.

ab117991 has been referenced in 7 publications.

  • Flax J  et al. OGA Inhibition Alters Energetics and Nutrient Sensing in Alzheimer's Disease Cytoplasmic Hybrids. J Alzheimers Dis 78:1743-1753 (2020). PubMed: 33285636
  • Carraro M  et al. The Unique Cysteine of F-ATP Synthase OSCP Subunit Participates in Modulation of the Permeability Transition Pore. Cell Rep 32:108095 (2020). PubMed: 32877677
  • Kovalcíková J  et al. TMEM70 facilitates biogenesis of mammalian ATP synthase by promoting subunit c incorporation into the rotor structure of the enzyme. FASEB J 33:14103-14117 (2019). PubMed: 31652072
  • Ihara M  et al. An interaction between glucagon-like peptide-1 and adenosine contributes to cardioprotection of a dipeptidyl peptidase 4 inhibitor from myocardial ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol 308:H1287-97 (2015). PubMed: 25747753
  • Kimura T  et al. Autophagy protects kidney proximal tubule epithelial cells from mitochondrial metabolic stress. Autophagy 9:1876-86 (2013). PubMed: 24128672
  • Milenkovic D  et al. TWINKLE is an essential mitochondrial helicase required for synthesis of nascent D-loop strands and complete mtDNA replication. Hum Mol Genet 22:1983-93 (2013). WB ; Mouse . PubMed: 23393161
  • Croston TL  et al. Evaluation of the cardiolipin biosynthetic pathway and its interactions in the diabetic heart. Life Sci 93:313-22 (2013). PubMed: 23872101

Customer reviews and Q&As

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Question

Q: Doesnt antigen retrieval destroy the cells thus biasing results?

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Answer

Thank you for your question submitted during our ICC webinar last week. We hope you enjoyed the presentation and found this useful.

Our sister company, Mitosciences, often uses antigen retrieval with ICC testing of the antibodies we sell. I contacted them for further information and can confirm the following:

In their experience they have not seen destruction of cell morphology during antigen retrieval. The cell lines they have typically used at MitoSciences are HdFn, HeLa, HepG2, MRC5, OXPHOS deficient skin fibroblasts. It is possible thatsome cell linesmay be too fragile for this procedure, but for those tested so far there have been no problems. They have not used other methods of antigen retrieval with this procedure.

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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