Anti-ATP5H antibody [10G5AB9] (ab173006)
![Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)](https://www.abcam.com/ps/products/173/ab173006/Images/ab173006-196454-anti-atp5h-antibody-10g5ab9-western-blot.jpg "Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)")
Key features and details
- Mouse monoclonal [10G5AB9] to ATP5H
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human, African green monkey
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-ATP5H antibody [10G5AB9]
See all ATP5H primary antibodies -
Description
Mouse monoclonal [10G5AB9] to ATP5H -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human, African green monkey -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Human, Rat, Mouse and Bovine Heart Homogenates; HeLa, HepG2, H4IIE, MEF, NIH-3T3 and COS7 cell lysates; HDFn cells; HeLa cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
10G5AB9 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 18 kDa.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 18 kDa. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. -
Sequence similarities
Belongs to the ATPase d subunit family. -
Cellular localization
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 282710 Cow
- Entrez Gene: 10476 Human
- Entrez Gene: 71679 Mouse
- Entrez Gene: 641434 Rat
- SwissProt: P13620 Cow
- SwissProt: O75947 Human
- SwissProt: Q9DCX2 Mouse
- SwissProt: P31399 Rat
see all -
Alternative names
- ATP synthase D chain mitochondrial antibody
- ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
- ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
see all
Images
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Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)All lanes : Anti-ATP5H antibody [10G5AB9] (ab173006) at 0.200000002980232 µg/ml
Lane 1 : Human Heart Homogenate at 20 µg
Lane 2 : Rat Heart Homogenate at 20 µg
Lane 3 : Mouse Heart Homogenate at 20 µg
Lane 4 : Bovine Heart Homogenate at 15 µg
Lane 5 : Hela cell lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution
Predicted band size: 18 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB9] (ab173006)](https://www.abcam.com/ps/products/173/ab173006/Images/ab173006-196461-anti-atp5h-antibody-10g5ab9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB9] (ab173006)Immunocytochemistry with HDFn cells stained with ab173006 (red) and DAPI (blue).
Fixation: Paraformaldehyde fixed (4%, 20 min).
Used heated antigen retrieval for optimal signal.
Permeabilization: Triton X-100 permeabilized (0.1%, 10min).
Blocking: 10% Goat Serum/PBS (1 hour).
Primary: ab173006 5 µg/ml (10% Goat Serum/PBS, 2hr at room temperature or over night at 4°C).
Secondary: Alexa Fluor® 594 goat anti-mouse H+L used at a 1/1000 dilution in 1% Goat Serum/PBS for 1hr at RT.
Washing: 3X 1% Goat Serum/PBS, 10 mins/wash.
DAPI: 2 µM in 1% Goat Serum/PBS, 10 mins.
The target protein locates to the mitochondria (red).
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![Flow Cytometry - Anti-ATP5H antibody [10G5AB9] (ab173006)](https://www.abcam.com/ps/products/173/ab173006/Images/ab173006-196462-anti-atp5h-antibody-10g5ab9-flow-cytometry.jpg)
Flow Cytometry - Anti-ATP5H antibody [10G5AB9] (ab173006)Flow Cytometry with HeLa cells stained with ab173006 (blue) and isotype-specific negative control (red).
Fixation: Paraformaldehyde fixed (4%, 15 min).
Permeabilization: Methanol permeabilized (90%, @ -20°C for >30 min).
Blocking: 1% BSA /PBS (20 min).
Primary: ab173006 0.5 µg/ml in 1% BSA/PBS, 1hr at room temperature.
Secondary: Alexa Fluor® 488 goat anti-mouse IgG1 used at a 1/1000 dilution in 1% BSA/PBS for 30 min at RT.
Washing: 2x 1% BSA/PBS.
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![Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)](https://www.abcam.com/ps/products/173/ab173006/Images/ab173006-196457-anti-atp5h-antibody-10g5ab9-western-blot.jpg)
Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)All lanes : Anti-ATP5H antibody [10G5AB9] (ab173006) at 0.5 µg/ml
Lane 1 : HepG2 cell lysate at 20 µg
Lane 2 : H4IIE cell lysate at 20 µg
Lane 3 : MEF cell lysate at 20 µg
Lane 4 : NIH-3T3 cell lysate at 20 µg
Lane 5 : COS7 cell lysate at 10 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution
Predicted band size: 18 kDa
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab173006 has been referenced in 1 publication.
- Zhao Y et al. Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes. BMC Genomics 20:569 (2019). PubMed: 31291894
