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Anti-ATP5H antibody [7F9BG1] (ab110275)

Reviews (3)Q&A (1)References (16)
Western blot - Anti-ATP5H antibody [7F9BG1] (ab110275)
  • Western blot - Anti-ATP5H antibody [7F9BG1] (ab110275)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [7F9BG1] (ab110275)
  • Flow Cytometry - Anti-ATP5H antibody [7F9BG1] (ab110275)

Key features and details

  • Mouse monoclonal [7F9BG1] to ATP5H
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Cow, Human, African green monkey
  • Isotype: IgG2b

Overview

  • Product name

    Anti-ATP5H antibody [7F9BG1]
    See all ATP5H primary antibodies

  • Description

    Mouse monoclonal [7F9BG1] to ATP5H

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, African green monkey

  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells

  • General notes

    For mouse and rat samples, this antibody will only recognize ATP5H in purified mitochondrial samples. Mouse and rat cell lysates and tissue homogenates are not recommneded with this antibody.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa.
ICC/IF
Use a concentration of 1 - 5 µg/ml.

(heat-induced antigen-retrieval improvessignal)
Flow Cyt (1)
Use a concentration of 1 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa.
ICC/IF
Use a concentration of 1 - 5 µg/ml.

(heat-induced antigen-retrieval improvessignal)
Flow Cyt
Use a concentration of 1 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 

Target

  • Function

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

  • Sequence similarities

    Belongs to the ATPase d subunit family.

  • Cellular localization

    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ATP synthase D chain mitochondrial antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
    • ATP synthase subunit d antibody
    • ATP synthase subunit d, mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody
    • ATP5H antibody
    • ATP5H_HUMAN antibody
    • ATP5JD antibody
    • ATPase subunit d antibody
    • ATPQ antibody
    • mitochondrial antibody
    • My032 protein antibody
    see all

Images

  • Western blot - Anti-ATP5H antibody [7F9BG1] (ab110275)
    Western blot - Anti-ATP5H antibody [7F9BG1] (ab110275)
    All lanes : Anti-ATP5H antibody [7F9BG1] (ab110275) at 1 µg/ml

    Lane 1 : HDFn (human) cell lysates at 20 µg
    Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysates at 20 µg
    Lane 3 : H4IIE (rat) cell lysates at 20 µg
    Lane 4 : MEF (mouse) cell lysates at 20 µg
    Lane 5 : bovine heart mitochondria lysates at 5 µg

    Predicted band size: 18 kDa

  • Western blot - Anti-ATP5H antibody [7F9BG1] (ab110275)
    Western blot - Anti-ATP5H antibody [7F9BG1] (ab110275)
    All lanes : Anti-ATP5H antibody [7F9BG1] (ab110275) at 1/1 dilution

    Lane 1 : Human heart mitochondria at 10 µg
    Lane 2 : Bovine heart mitochondria at 4 µg
    Lane 3 : Rat heart mitochondria at 10 µg
    Lane 4 : Mouse heart mitochondria at 10 µg
    Lane 5 : HepG2 mitochondria at 20 µg

    Predicted band size: 18 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [7F9BG1] (ab110275)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [7F9BG1] (ab110275)
    Mitochondrial localization of ATP5H. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110275 (5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG(H+L) secondary antibody (2 µg/ml).
  • Flow Cytometry - Anti-ATP5H antibody [7F9BG1] (ab110275)
    Flow Cytometry - Anti-ATP5H antibody [7F9BG1] (ab110275)
    HeLa cells were stained with 1 µg/mL ab110275 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

References (16)

Publishing research using ab110275? Please let us know so that we can cite the reference in this datasheet.

ab110275 has been referenced in 16 publications.

  • Lewis CJ  et al. Codon optimization is an essential parameter for the efficient allotopic expression of mtDNA genes. Redox Biol 30:101429 (2020). WB ; Human . PubMed: 31981894
  • Ng R  et al. Contractile work directly modulates mitochondrial protein levels in human engineered heart tissues. Am J Physiol Heart Circ Physiol 318:H1516-H1524 (2020). PubMed: 32383992
  • Chaphalkar RM  et al. Endothelin-1 Mediated Decrease in Mitochondrial Gene Expression and Bioenergetics Contribute to Neurodegeneration of Retinal Ganglion Cells. Sci Rep 10:3571 (2020). PubMed: 32107448
  • Vilella R  et al. Effects of Standardized Green Tea Extract and Its Main Component, EGCG, on Mitochondrial Function and Contractile Performance of Healthy Rat Cardiomyocytes. Nutrients 12:N/A (2020). PubMed: 32993022
  • Pecina P  et al. Role of the mitochondrial ATP synthase central stalk subunits ? and d in the activity and assembly of the mammalian enzyme. Biochim Biophys Acta 1859:374-381 (2018). PubMed: 29499186
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-ATP5H antibody [7F9BG1]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (CaSki(cervical cancer))
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
20 µg
Specification
CaSki(cervical cancer)
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Suyeon Kim

Verified customer

Submitted Jan 26 2018

Flow Cytometry abreview for Anti-ATP5H [7F9BG1] antibody

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Fixation
Beckman Coulter PerFix-nc
Permeabilization
Yes - Beckman Coulter PerFix-nc
Sample
Rhesus monkey Cell (MSC)
Specification
MSC
Gating Strategy
The initial gate is set for total bone marrow
Preparation
Cell harvesting/tissue preparation method: Isolated and expanded MSCs
Sample buffer: MEM + 20% FBS
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 17 2013

Western blot abreview for Anti-ATP5H [7F9BG1] antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 29#T)
Loading amount
20 µg
Specification
HEK 29#T
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 01 2011

Question

I would like to know the fixation method you use for Immunocitochemistry. Do you use PFA, methanol?

Read More
Answer



For this antibody we have used a protocol with PFA and then a permeabilisation step with TritonX:

1. Fix the cells by incubating them in 4% (v/v) paraformaldehyde in PBS for 20 minutes at room

temperature.

2. Rinse the cells three times with PBS. The cells can be stored in 0.02% (w/v) sodium azide in PBS

at 4°C for several days.

3. Incubate the cells in 0.1% Triton X-100 in PBS for 15 minutes at room temperature.

4. Rinse the cells 3 times in PBS.

Read More

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