Anti-ATP6V1A antibody [8B1F3] (ab113745)
![Western blot - Anti-ATP6V1A antibody [8B1F3] (ab113745)](https://www.abcam.com/ps/products/113/ab113745/Images/ab113745-126444-anti-atp6v1a-antibody-8b1f3-western-blot.jpg "Western blot - Anti-ATP6V1A antibody [8B1F3] (ab113745)")
Key features and details
- Mouse monoclonal [8B1F3] to ATP6V1A
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG2a
Overview
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Product name
Anti-ATP6V1A antibody [8B1F3]
See all ATP6V1A primary antibodies -
Description
Mouse monoclonal [8B1F3] to ATP6V1A -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation. -
Clonality
Monoclonal -
Clone number
8B1F3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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| Notes |
|---|
|
WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
Target
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Function
Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. -
Tissue specificity
Present in all tissues analyzed. -
Sequence similarities
Belongs to the ATPase alpha/beta chains family. - Information by UniProt
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Alternative names
- 70kDa subunit antibody
- ATP6A1 antibody
- ATP6V1A antibody
see all
Images
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Western blot - Anti-ATP6V1A antibody [8B1F3] (ab113745)Anti-ATP6V1A antibody [8B1F3] (ab113745) at 1 µg/ml + Yeast Whole Cell at 15 µg
Predicted band size: 69 kDa
Datasheets and documents
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Datasheet download
References (2)
ab113745 has been referenced in 2 publications.
- Chandel A & Bachhawat AK Redox regulation of the yeast voltage-gated Ca2+ channel homolog Cch1p by glutathionylation of specific cysteine residues. J Cell Sci 130:2317-2328 (2017). PubMed: 28576969
- Chandel A et al. Glutathione depletion activates the yeast vacuolar transient receptor potential channel, Yvc1p, by reversible glutathionylation of specific cysteines. Mol Biol Cell 27:3913-3925 (2016). IF . PubMed: 27708136
