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RecombinantRabMAb

Recombinant Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)

Submit a review Submit a question References (9)
Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
  • Western blot - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
  • Western blot - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
  • Western blot - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
  • Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16401] to ATP6V1B1 + ATP6V1B2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401]
    See all ATP6V1B1 + ATP6V1B2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16401] to ATP6V1B1 + ATP6V1B2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: JAR and RAW 264.7 cell lysates; Mouse brain, mouse kidney and rat kidney tissue lysates. IHC-P: Human, mouse and rat kidney tissues. ICC/IF: HEK293 and JAR cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16401

  • Isotype

    IgG

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab200839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P
1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
Notes
WB
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P
1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized JAR (Human placenta choriocarcinoma cell line) cells labeling ATP6V1B1 + ATP6V1B2 with ab200839 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic staining on JAR cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab200839 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human embryonic kidney) cells labeling ATP6V1B1 + ATP6V1B2 with ab200839 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic staining on HEK293 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab200839 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Western blot - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
    Western blot - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
    Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839) at 1/5000 dilution + JAR (Human placenta choriocarcinoma cell line) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 57 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
    Western blot - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
    All lanes : Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 57 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
    Western blot - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
    All lanes : Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839) at 1/1000 dilution

    Lane 1 : JAR (Human placenta choriocarcinoma cell line) cell lysate
    Lane 2 : JAR (Human placenta choriocarcinoma cell line) cell lysate with immunizing peptide
    Lane 3 : JAR (Human placenta choriocarcinoma cell line) cell lysate with ATP6V1B2 peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 57 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ATP6V1B1 + ATP6V1B2 with ab200839 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling ATP6V1B1 + ATP6V1B2 with ab200839 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATP6V1B1 + ATP6V1B2 with ab200839 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on rat kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling ATP6V1B1 + ATP6V1B2 with ab200839 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human cardiac muscle tissue represents a negative control for ATP6V1B1 + ATP6V1B2. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)
    Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)

References (9)

Publishing research using ab200839? Please let us know so that we can cite the reference in this datasheet.

ab200839 has been referenced in 9 publications.

  • Sivaraj KK  et al. Mesenchymal stromal cell-derived septoclasts resorb cartilage during developmental ossification and fracture healing. Nat Commun 13:571 (2022). PubMed: 35091558
  • Xu C  et al. Induction of osteogenesis by bone-targeted Notch activation. Elife 11:N/A (2022). PubMed: 35119364
  • Gong QQ  et al. A novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter. PLoS One 16:e0254802 (2021). PubMed: 34310634
  • Xue P  et al. PGE2/EP4 skeleton interoception activity reduces vertebral endplate porosity and spinal pain with low-dose celecoxib. Bone Res 9:36 (2021). PubMed: 34334792
  • Sivaraj KK  et al. YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells. Elife 9:N/A (2020). PubMed: 31958058
  • Leir SH  et al. An atlas of human proximal epididymis reveals cell-specific functions and distinct roles for CFTR. Life Sci Alliance 3:N/A (2020). PubMed: 32855272
  • Leir SH  et al. An organoid model to assay the role of CFTR in the human epididymis epithelium. Cell Tissue Res 381:327-336 (2020). PubMed: 32377875
  • Li M  et al. Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK. Cell Metab 30:508-524.e12 (2019). PubMed: 31204282
  • Song XB  et al. Autophagy blockade and lysosomal membrane permeabilization contribute to lead-induced nephrotoxicity in primary rat proximal tubular cells. Cell Death Dis 8:e2863 (2017). PubMed: 28594408

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