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Anti-ATPB antibody [7E3F2] (ab110280)

Reviews (1)Q&A (3)References (6)
Western blot - Anti-ATPB antibody [7E3F2] (ab110280)

    Key features and details

    • Mouse monoclonal [7E3F2] to ATPB
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Cow, Human
    • Isotype: IgG2a

    Overview

    • Product name

      Anti-ATPB antibody [7E3F2]
      See all ATPB primary antibodies

    • Description

      Mouse monoclonal [7E3F2] to ATPB

    • Host species

      Mouse

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse, Rat, Cow, Human

    • Immunogen

      Full length native protein (purified). This information is considered to be commercially sensitive.

    • Positive control

      • Isolated mitochondria from Human heart, bovine heart, rat heart, mouse heart, and HepG2 cells.

    • General notes

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

      Product was previously marketed under the MitoSciences sub-brand.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab110280 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 4 µg/ml. Predicted molecular weight: 57 kDa.
    Notes
    WB
    Use a concentration of 4 µg/ml. Predicted molecular weight: 57 kDa.

    Target

    • Function

      Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

    • Sequence similarities

      Belongs to the ATPase alpha/beta chains family.

    • Cellular localization

      Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ATP 5B antibody
      • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
      • ATP synthase subunit beta mitochondrial antibody
      • ATP synthase subunit beta, mitochondrial antibody
      • atp5b antibody
      • ATPB antibody
      • ATPB_HUMAN antibody
      • ATPMB antibody
      • ATPSB antibody
      • Epididymis secretory protein Li 271 antibody
      • HEL-S-271 antibody
      • Mitochondrial ATP synthase beta subunit antibody
      • Mitochondrial ATP Synthase Subunit Beta antibody
      • Mitochondrial ATP synthetase beta subunit antibody
      see all

    Images

    • Western blot - Anti-ATPB antibody [7E3F2] (ab110280)
      Western blot - Anti-ATPB antibody [7E3F2] (ab110280)
      All lanes : Anti-ATPB antibody [7E3F2] (ab110280) at 4 µg/ml

      Lane 1 : Isolated mitochondria from Human heart at 15 µg
      Lane 2 : Isolated mitochondria from cow heart at 6 µg
      Lane 3 : Isolated mitochondria from rat heart at 30 µg
      Lane 4 : Isolated mitochondria from mouse heart at 30 µg
      Lane 5 : Isolated mitochondria from HepG2 cells at 30 µg

      Predicted band size: 57 kDa

    References (6)

    Publishing research using ab110280? Please let us know so that we can cite the reference in this datasheet.

    ab110280 has been referenced in 6 publications.

    • Yeom J  et al. Small proteins regulate Salmonella survival inside macrophages by controlling degradation of a magnesium transporter. Proc Natl Acad Sci U S A 117:20235-20243 (2020). PubMed: 32753384
    • Hou X  et al. Testosterone disruptor effect and gut microbiome perturbation in mice: Early life exposure to doxycycline. Chemosphere 222:722-731 (2019). PubMed: 30738315
    • Park HE  et al. Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio. Adv Sci (Weinh) 6:1901673 (2019). PubMed: 31763149
    • Yeom J  et al. Sequestration from Protease Adaptor Confers Differential Stability to Protease Substrate. Mol Cell 66:234-246.e5 (2017). PubMed: 28431231
    • Yavlovich A  et al. Ectopic ATP synthase facilitates transfer of HIV-1 from antigen-presenting cells to CD4(+) target cells. Blood 120:1246-53 (2012). PubMed: 22753871
    • Kim M  et al. Increased expression of the F(1)F(o) ATP synthase in response to iron in heart mitochondria. BMB Rep 41:153-7 (2008). WB ; Rat . PubMed: 18315952

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-ATPB antibody [7E3F2]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Bacteria Cell lysate - whole cell (Sinorhizobium meliloti whole cell lysate)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    3e+007 cells
    Treatment
    in SDS sample buffer 2-mercaptoethanol
    Specification
    Sinorhizobium meliloti whole cell lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Joseph Chen

    Verified customer

    Submitted Mar 06 2017

    Question

    Sorry for the delayed reply. We would like to proceed and test ab110280 with Acinetobacter baumannii.

    Read More
    Answer

    I am very pleased to hear you would like to accept our offer and test ab110280 in Acinetobacter baumannii.. This code will give you one free antibody before the expiration date. To redeem this offer, please submit an Abreview for Acinetobacter baumannii and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    We publish both positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Question

    We are interested in an anti-AtpB antibody to detect AtpB of the bacterium Acinetobacter baumannii. We see that you sell an Anti-ATPB antibody [7E3F2] (ab110280) that recognizes Escherichia coli AtpB. Do you know if it reacts with Acinetobacter baumannii AtpB? If you do not know, we were wondering if you might be able to provide us with a small sample in order to determine if it reacts with Acinetobacter baumannii AtpB. If it does, we intend to purchase this antibody in the future; however, we would like to avoid spending the money on an antibody that may not work for us. If you would like, we can let you know if it does work for your product reference sheet. We would be very appreciative of your help.

    Read More
    Answer

    Thank you very much for your interest in ab110280.

    To our knowledge, ab110280 has not been tested against samples of Acinetobacter baumannii . Therefore, I can offer a discount off a future purchase if you buy ab110280 now, test it in Acinetobacter baumannii, and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of one free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab110280 with Acinetobacter baumannii. I will then send a discount code. This code must be issued before purchasing ab110280 so please wait for my reply before ordering.

    2. Purchase ab110280 either by phone, fax, or online (www.abcam.com).

    3. Test it against Acinetobacter baumannii.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab110280 turns out to be unsuitable, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Question

    Thank you very much for the prompt response. I wonder of you also have human mitochondria similar to the bovine preparation? Also, please let me know if the anti ATP synthase that you sell (particularly the mouse monoclonal ab5432 and the monoclonal antobody clone 7E3F2 - AB110280) interact with the bovine mitochondria - AB110338. I thank you in advance for your answer as soon as you can.

    Read More
    Answer

    Thank you for your email. Yes, we do carry human mitochondrial lysates as well: Human Liver Mitochondrial lysate (ab110339) Human Heart Mitochondrial lysate (ab110337) However, both lysates come in WB loading buffer (ready to be used in WB) and not iso-osmotic buffer as the bovine lysate. Regarding the antibodies: ab5432: This antibody has not been tested with bovine samples yet, but the sequence homology between rat (immunogen) and bovine is 96%. If you would like to try the antibody in bovine, you can take advantage of our testing discount program. For details please see below. ab110280: This antibody has been tested with bovine samples in WB and would be guaranteed for this species and application. Please see lane 2 for the WB data with bovine mitochondria. *Testing discount program: Here is some information about how the testing discount program works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. *Abpromise guarantee: All our products are guaranteed for the tested species and applications as listed on the datasheet for 6 months after the purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com