Anti-Azurocidin antibody (ab167336)
Key features and details
- Mouse polyclonal to Azurocidin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Azurocidin antibody
See all Azurocidin primary antibodies -
Description
Mouse polyclonal to Azurocidin -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Azurocidin aa 1-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human spleen lysate; Azurocidin transfected 293T cell lysate; Human lymph node tissue; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
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| IHC-P |
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. |
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IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Cytoplasmic granule. Cytoplasmic granules of neutrophils. - Information by UniProt
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Database links
- Entrez Gene: 566 Human
- Omim: 162815 Human
- SwissProt: P20160 Human
- Unigene: 72885 Human
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Alternative names
- Azu 1 antibody
- AZU1 antibody
- Azurocidin antibody
see all
Images
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Western blot - Anti-Azurocidin antibody (ab167336)Anti-Azurocidin antibody (ab167336) at 1 µg/ml + Human spleen lysate at 50 µg
Secondary
Goat Anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 26 kDa -
Western blot - Anti-Azurocidin antibody (ab167336)All lanes : Anti-Azurocidin antibody (ab167336) at 1 µg/ml
Lane 1 : Azurocidin transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 26 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Azurocidin antibody (ab167336)Immunofluorescent analysis of Azurocidin expression in HeLa cells using ab167336 at 10 µg/ml dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody (ab167336)Immunohistochemical analysis of Azurocidin expression in formalin-fixed paraffin-embedded Human lymph node tissue using ab167336 at 3 µg/ml dilution
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab167336 has been referenced in 2 publications.
- Naito T et al. Azurocidin is loaded into small extracellular vesicles via its N-linked glycosylation and promotes intravasation of renal cell carcinoma cells. FEBS Lett 595:2522-2532 (2021). PubMed: 34418081
- Mayer P et al. Changes in the microarchitecture of the pancreatic cancer stroma are linked to neutrophil-dependent reprogramming of stellate cells and reflected by diffusion-weighted magnetic resonance imaging. Theranostics 8:13-30 (2018). PubMed: 29290790
