Recombinant Anti-BACE1 antibody [027] - BSA and Azide free (ab277127)
![Flow Cytometry (Intracellular) - Anti-BACE1 antibody [027] - BSA and Azide free (ab277127)](https://www.abcam.com/ps/products/277/ab277127/Images/ab277127-2-AntiBACE1-antibody-Flow-cyt-Jurkat-cells.jpg "Flow Cytometry (Intracellular) - Anti-BACE1 antibody [027] - BSA and Azide free (ab277127)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [027] to BACE1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC
- Reacts with: Human
Overview
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Product name
Anti-BACE1 antibody [027] - BSA and Azide free
See all BACE1 primary antibodies -
Description
Rabbit monoclonal [027] to BACE1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Human BACE1 aa 1-457 (C terminal). Genbank: NP_036236.1
Database link: P56817 -
Positive control
- Flow Cyt (Intra): Jurkat cells. ICC: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS
0.2 µm filtered solution -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
027 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab277127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/25 - 1/100.
|
|
| ICC |
1/20 - 1/100.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/25 - 1/100. |
|
ICC
1/20 - 1/100. |
Target
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Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. -
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. -
Sequence similarities
Belongs to the peptidase A1 family. -
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 23621 Human
- Omim: 604252 Human
- SwissProt: P56817 Human
- Unigene: 504003 Human
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Alternative names
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-BACE1 antibody [027] - BSA and Azide free (ab277127)
Intracellular flow cytometric analysis of BACE1 expression on Jurkat (human T cell leukemia cell line from peripheral blood) cells. The cells were stained with ab277127 at 1/25 dilution, then a FITC-conjugated second step antibody (Black). Cells were also stained with an isotype control (Grey). The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Immunocytochemistry - Anti-BACE1 antibody (ab277127)Immunofluorescence staining of BACE1 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells. Cells were fixed with 4% PFA, permeabilized with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab277127 at 1/60 dilution at 37°C for 1 hour. Then cells were stained with the AlexaFluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
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![Anti-BACE1 antibody [027] - BSA and Azide free (ab277127)](https://www.abcam.com/ps/products/277/ab277127/Images/ab277127-3-benefits-of-recombinant-antibodies.png)
Anti-BACE1 antibody [027] - BSA and Azide free (ab277127)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab277127 has not yet been referenced specifically in any publications.
