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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-BACE1 antibody [EPR22802-233] (ab263901)

Reviews (1) Submit a question
Western blot - Anti-BACE1 antibody [EPR22802-233] (ab263901)
  • Western blot - Anti-BACE1 antibody [EPR22802-233] (ab263901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] (ab263901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] (ab263901)
  • Anti-BACE1 antibody [EPR22802-233] (ab263901)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22802-233] to BACE1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-BACE1 antibody [EPR22802-233]
    See all BACE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22802-233] to BACE1

  • Host species

    Rabbit

  • Specificity

    This antibody is not recommended for human IHC-P

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human BACE1 recombinant protein; Wild-type HAP1 and SH-SY5Y whole cell lysates; Mouse brain, Human brain and Rat hippocampus lysates. IHC-P: Mouse hippocampus and Rat hippocampus tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab263901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This antibody is not recommended for human IHC-P
Notes
WB
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This antibody is not recommended for human IHC-P
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.

  • Tissue specificity

    Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.

  • Sequence similarities

    Belongs to the peptidase A1 family.

  • Domain

    The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.

  • Post-translational
    modifications

    Glycosylated.

  • Cellular localization

    Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • APP beta secretase antibody
    • Asp 2 antibody
    • ASP2 antibody
    • Aspartyl protease 2 antibody
    • BACE 1 antibody
    • BACE antibody
    • BACE1 antibody
    • BACE1_HUMAN antibody
    • Beta secretase 1 antibody
    • Beta secretase antibody
    • Beta site amyloid beta A4 precursor protein cleaving enzyme antibody
    • Beta site amyloid precursor protein cleaving enzyme 1 antibody
    • Beta site amyloid precursor protein cleaving enzyme antibody
    • Beta site APP cleaving enzyme 1 antibody
    • Beta site APP cleaving enzyme antibody
    • Beta-secretase 1 antibody
    • Beta-site amyloid precursor protein cleaving enzyme 1 antibody
    • Beta-site APP cleaving enzyme 1 antibody
    • FLJ90568 antibody
    • HSPC104 antibody
    • Memapsin 2 antibody
    • Memapsin-2 antibody
    • Memapsin2 antibody
    • Membrane associated aspartic protease 2 antibody
    • Membrane-associated aspartic protease 2 antibody
    • Transmembrane aspartic proteinase Asp2 antibody
    see all

Images

  • Western blot - Anti-BACE1 antibody [EPR22802-233] (ab263901)
    Western blot - Anti-BACE1 antibody [EPR22802-233] (ab263901)
    All lanes : Anti-BACE1 antibody [EPR22802-233] (ab263901) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : BACE1 knockout HAP1 whole cell lysate
    Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Human brain lysate
    Lane 6 : Rat hippocampus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-2:26 seconds;
    Lanes 3-6:10 seconds.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17425515).

    ab263901 was shown to specifically react with BACE1 in wild-type HAP1 cells as signal was lost in BACE1 knockout cells. Wild-type and BACE1 knockout samples were subjected to SDS-PAGE. ab263901 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

  • Western blot - Anti-BACE1 antibody [EPR22802-233] (ab263901)
    Western blot - Anti-BACE1 antibody [EPR22802-233] (ab263901)
    Anti-BACE1 antibody [EPR22802-233] (ab263901) at 1/1000 dilution + His-tagged human BACE1 recombinant protein (aa22-460 &aa46-460), 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa
    Observed band size: 65-67,68-70 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] (ab263901)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] (ab263901)

    Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling BACE1 with ab263901 at 1/4000 dilution (0.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mossy fibers in the hilar region of the dentate gyrus. The section was incubated with ab263901 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] (ab263901)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] (ab263901)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling BACE1 with ab263901 at 1/4000 dilution (0.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mossy fibers in the hilar region of the dentate gyrus. The section was incubated with ab263901 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)..

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Anti-BACE1 antibody [EPR22802-233] (ab263901)
    Anti-BACE1 antibody [EPR22802-233] (ab263901)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab263901? Please let us know so that we can cite the reference in this datasheet.

ab263901 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Western blot abreview for Anti-BACE1 antibody [EPR22802-233]

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Axolotl Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Mar 17 2023

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