Recombinant Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)
![Western blot - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)](https://www.abcam.com/ps/products/267/ab267796/Images/ab267796-367689-ab2639014antibace1antibodyepr22802233westernblot.jpg "Western blot - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22802-233] to BACE1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free
See all BACE1 primary antibodies -
Description
Rabbit monoclonal [EPR22802-233] to BACE1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged human BACE1 recombinant protein; Wild-type HAP1 and SH-SY5Y whole cell lysates; Mouse brain, Human brain and Rat hippocampus lysates. IHC-P: Mouse hippocampus and Rat hippocampus tissues.
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General notes
ab267796 is the carrier-free version of ab263901.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22802-233 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab267796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This antibody is not recommended for human IHC-P |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa). |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. This antibody is not recommended for human IHC-P |
Target
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Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. -
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. -
Sequence similarities
Belongs to the peptidase A1 family. -
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 23621 Human
- Entrez Gene: 23821 Mouse
- Entrez Gene: 29392 Rat
- Omim: 604252 Human
- SwissProt: P56817 Human
- SwissProt: P56818 Mouse
- SwissProt: P56819 Rat
- Unigene: 504003 Human
see all -
Alternative names
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
see all
Images
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Western blot - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)All lanes : Anti-BACE1 antibody [EPR22802-233] (ab263901) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : BACE1 knockout HAP1 whole cell lysate
Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 4 : Mouse brain lysate
Lane 5 : Human brain lysate
Lane 6 : Rat hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lanes 1-2:26 seconds;
Lanes 3-6:10 seconds.The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17425515).
ab263901 was shown to specifically react with BACE1 in wild-type HAP1 cells as signal was lost in BACE1 knockout cells. Wild-type and BACE1 knockout samples were subjected to SDS-PAGE. ab263901 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263901).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)](https://www.abcam.com/ps/products/267/ab267796/Images/ab267796-2-anti-bace1-antibody-epr22802-233-immunohistochemistry-hippocampus-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling BACE1 with ab263901 at 1/4000 dilution (0.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mossy fibers in the hilar region of the dentate gyrus. The section was incubated with ab263901 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263901).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)](https://www.abcam.com/ps/products/267/ab267796/Images/ab267796-1-anti-bace1-antibody-epr22802-233-immunohistochemistry-hippocampus-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling BACE1 with ab263901 at 1/4000 dilution (0.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mossy fibers in the hilar region of the dentate gyrus. The section was incubated with ab263901 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)..
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263901).
-
![Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)](https://www.abcam.com/ps/products/267/ab267796/Images/ab267796-4-benefits-of-recombinant-antibodies.png)
Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab267796 has not yet been referenced specifically in any publications.
