Recombinant Anti-BACE2 antibody [EPR23339-221] (ab270458)
![Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)](https://www.abcam.com/ps/products/270/ab270458/Images/ab270458-514386-antibace2antibodyepr23339221westernblotmouse.jpg "Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23339-221] to BACE2
- Suitable for: IHC-Fr, WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-BACE2 antibody [EPR23339-221]
See all BACE2 primary antibodies -
Description
Rabbit monoclonal [EPR23339-221] to BACE2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, A549, C6, Beta-TC-6 and Neuro 2a cell lysate. IHC-P: Mouse and rat pancreas tissue. IHC-fr: Mouse pancreas. ICC/IF: Beta-TC-6 cells. IP: Neuro-2a whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23339-221 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/100.
|
|
| WB | (1) |
1/1000. Predicted molecular weight: 56 kDa.
|
| ICC/IF |
1/50.
100% methanol fixation is recommended. |
|
| IP |
1/30.
|
|
| IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IHC-Fr
1/100. |
|
WB
1/1000. Predicted molecular weight: 56 kDa. |
|
ICC/IF
1/50. 100% methanol fixation is recommended. |
|
IP
1/30. |
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. -
Tissue specificity
Brain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate. -
Sequence similarities
Belongs to the peptidase A1 family. -
Post-translational
modificationsUndergoes autoproteolytic cleavage.
Glycosylated. -
Cellular localization
Membrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface. - Information by UniProt
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Database links
- Entrez Gene: 25825 Human
- Entrez Gene: 56175 Mouse
- Entrez Gene: 288227 Rat
- Omim: 605668 Human
- SwissProt: Q9Y5Z0 Human
- SwissProt: Q9JL18 Mouse
- SwissProt: Q6IE75 Rat
- Unigene: 529408 Human
see all -
Alternative names
- ALP 56 antibody
- ALP56 antibody
- Asp 1 antibody
see all
Images
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Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)All lanes : Anti-BACE2 antibody [EPR23339-221] (ab270458) at 1/1000 dilution
Lane 1 : Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse hippocampus tissue
Lane 4 : Mouse cerebellum tissue
Lane 5 : Mouse cerebral cortex tissue
Lane 6 : Mouse spinal cord tissue
Lane 7 : Mouse midbrain tissue lysate
Lane 8 : Mouse forebrain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Brain has very low level of BACE2 [PMID: 10749877, PMID: 30626751].
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![Immunohistochemistry (Frozen sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)](https://www.abcam.com/ps/products/270/ab270458/Images/ab270458-5-anti-bace2-antibody-epr23339221-immunohistochemistry-frozen-mouse-pancreas.jpg)
Immunohistochemistry (Frozen sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling BACE2 with ab270458 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)](https://www.abcam.com/ps/products/270/ab270458/Images/ab270458-1-anti-bace2-antibody-epr23339221-immunohistochemistry-mouse-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling BACE2 with ab270458 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse pancreatic islet (PMID: 21907142). The section was incubated with ab270458 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)](https://www.abcam.com/ps/products/270/ab270458/Images/ab270458-6-anti-bace2-antibody-epr23339221-western-blot-lysate.jpg)
Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)All lanes : Anti-BACE2 antibody [EPR23339-221] (ab270458) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 3 : C6 (rat glial tumor glial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 136 secs.
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![Immunoprecipitation - Anti-BACE2 antibody [EPR23339-221] (ab270458)](https://www.abcam.com/ps/products/270/ab270458/Images/ab270458-4-anti-bace2-antibody-epr23339221-immunoprecipitation-neuro2a.jpg)
Immunoprecipitation - Anti-BACE2 antibody [EPR23339-221] (ab270458)BACE2 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab270458 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270458 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Neuro-2a whole cell lysate.
Lane 2: ab270458 IP in Neuro-2a whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270458 in Neuro-2a whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 mins.
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![Immunocytochemistry/ Immunofluorescence - Anti-BACE2 antibody [EPR23339-221] (ab270458)](https://www.abcam.com/ps/products/270/ab270458/Images/ab270458-3-anti-bace2-antibody-epr23339221-immunocytochemistry-betatc6.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BACE2 antibody [EPR23339-221] (ab270458)Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 cells labeling BACE2 with ab270458 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic and membranous staining in Beta-TC-6 cell line. 100% methanol fixation is recommended. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)](https://www.abcam.com/ps/products/270/ab270458/Images/ab270458-2-anti-bace2-antibody-epr23339221-immunohistochemistry-rat-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling BACE2 with ab270458 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat pancreatic islet (PMID: 21907142). The section was incubated with ab270458 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)](https://www.abcam.com/ps/products/270/ab270458/Images/ab270458-7-anti-bace2-antibody-epr23339221-western-blot-lysate1.jpg)
Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)All lanes : Anti-BACE2 antibody [EPR23339-221] (ab270458) at 1/1000 dilution
Lane 1 : Beta-TC-6 (mouse pancreas insulinoma beta cell), whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 secs.
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![Anti-BACE2 antibody [EPR23339-221] (ab270458)](https://www.abcam.com/ps/products/270/ab270458/Images/ab270458-8-benefits-of-recombinant-antibodies.png)
Anti-BACE2 antibody [EPR23339-221] (ab270458)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab270458 has been referenced in 2 publications.
- Cao K et al. Activation of α7 Nicotinic Acetylcholine Receptor by its Selective Agonist Improved Learning and Memory of Amyloid Precursor Protein/Presenilin 1 (APP/PS1) Mice via the Nrf2/HO-1 Pathway. Med Sci Monit 28:e933978 (2022). PubMed: 34980874
- Susco SG et al. Molecular convergence between Down syndrome and fragile X syndrome identified using human pluripotent stem cell models. Cell Rep 40:111312 (2022). PubMed: 36070702