Recombinant Anti-BAFF antibody [EPR19704] - BSA and Azide free (ab251400)
![Western blot - Anti-BAFF antibody [EPR19704] - BSA and Azide free (ab251400)](https://www.abcam.com/ps/products/251/ab251400/Images/ab251400-408244-anti-baff-antibody-epr19704-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-BAFF antibody [EPR19704] - BSA and Azide free (ab251400)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19704] to BAFF - BSA and Azide free
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BAFF antibody [EPR19704] - BSA and Azide free
See all BAFF primary antibodies -
Description
Rabbit monoclonal [EPR19704] to BAFF - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251400 is the carrier-free version of ab203791.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19704 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 31, 29, 17 kDa (predicted molecular weight: 31 kDa).
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| IP |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 31, 29, 17 kDa (predicted molecular weight: 31 kDa). |
|
IP
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. -
Tissue specificity
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 10673 Human
- Omim: 603969 Human
- SwissProt: Q9Y275 Human
- Unigene: 525157 Human
- Unigene: 596110 Human
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Alternative names
- ApoL related ligand TALL 1 antibody
- B cell Activating Factor antibody
- B lymphocyte stimulator antibody
see all
Images
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Western blot - Anti-BAFF antibody [EPR19704] - BSA and Azide free (ab251400)All lanes : Anti-BAFF antibody [EPR19704] (ab203791) at 1/5000 dilution
Lane 1 : Empty vector with GFP-Myc tag (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) transfected with a GFP-Myc tagged BAFF vector whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis data was developed using ab203791, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-BAFF antibody [EPR19704] - BSA and Azide free (ab251400)](https://www.abcam.com/ps/products/251/ab251400/Images/ab251400-406405-anti-baff-antibody-epr19704-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-BAFF antibody [EPR19704] - BSA and Azide free (ab251400)Anti-BAFF antibody [EPR19704] (ab203791) at 1/1000 dilution + U937 (Human histiocytic lymphoma cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 17,29,31 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab203791, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Two isoforms have been described in literature (PMID: 12867412).
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![Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR19704] - BSA and Azide free (ab251400)](https://www.abcam.com/ps/products/251/ab251400/Images/ab251400-2-anti-baff-antibody-epr19704-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR19704] - BSA and Azide free (ab251400)This data was developed using ab203791, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (Human epithelial cell line from embryonic kidney) cells labeling BAFF with ab203791 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 647) (ab150079) secondary antibody at 1/1000 dilution. Confocal image showing positive staining on 293T cells transfected with a GFP-tagged BAFF expression construct. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150079 at 1/1000 dilution. ICC/IF was also performed on a few cell lines including U937, no signal was detected. -
![Immunoprecipitation - Anti-BAFF antibody [EPR19704] - BSA and Azide free (ab251400)](https://www.abcam.com/ps/products/251/ab251400/Images/ab251400-1-anti-baff-antibody-epr19704-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-BAFF antibody [EPR19704] - BSA and Azide free (ab251400)This data was developed using ab203791, the same antibody clone in a different buffer formulation.BAFF was immunoprecipitated from 0.35 mg of U937 (Human histiocytic lymphoma cell line) whole cell lysate with ab203791 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203791 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1: U937 whole cell lysate, 10µg (Input). Lane 2: ab203791 IP in U937 whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab203791 in U937 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds. -
![Anti-BAFF antibody [EPR19704] - BSA and Azide free (ab251400)](https://www.abcam.com/ps/products/251/ab251400/Images/ab251400-5-benefits-of-recombinant-antibodies.png)
Anti-BAFF antibody [EPR19704] - BSA and Azide free (ab251400)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251400 has not yet been referenced specifically in any publications.
