Recombinant Anti-BAFF antibody [EPR22238] (ab224710)
![Western blot - Anti-BAFF antibody [EPR22238] (ab224710)](https://www.abcam.com/ps/products/224/ab224710/Images/ab224710-337084-anti-baff-antibody-epr22238-western-blot.jpg "Western blot - Anti-BAFF antibody [EPR22238] (ab224710)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22238] to BAFF
- Suitable for: Flow Cyt, ICC/IF, IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BAFF antibody [EPR22238]
See all BAFF primary antibodies -
Description
Rabbit monoclonal [EPR22238] to BAFF -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IP, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U937 and HL-60 whole cell lysates. ICC/IF: U937 cells. Flow Cyt: HL-60 cells. IP: U937 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22238 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/500.
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| ICC/IF |
1/100.
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| IP |
1/30.
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| WB |
1/1000. Detects a band of approximately 30, 32 kDa (predicted molecular weight: 31 kDa).
|
| Notes |
|---|
|
Flow Cyt
1/500. |
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ICC/IF
1/100. |
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IP
1/30. |
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WB
1/1000. Detects a band of approximately 30, 32 kDa (predicted molecular weight: 31 kDa). |
Target
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Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. -
Tissue specificity
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 10673 Human
- Omim: 603969 Human
- SwissProt: Q9Y275 Human
- Unigene: 525157 Human
- Unigene: 596110 Human
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Alternative names
- ApoL related ligand TALL 1 antibody
- B cell Activating Factor antibody
- B lymphocyte stimulator antibody
see all
Images
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Western blot - Anti-BAFF antibody [EPR22238] (ab224710)All lanes : Anti-BAFF antibody [EPR22238] (ab224710) at 1/1000 dilution
Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 2 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 30,32 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-BAFF antibody [EPR22238] (ab224710)](https://www.abcam.com/ps/products/224/ab224710/Images/ab224710-337083-anti-baff-antibody-epr22238-immunoprecipitation.jpg)
Immunoprecipitation - Anti-BAFF antibody [EPR22238] (ab224710)BAFF was immunoprecipitated from 0.35 mg of U937 (human histiocytic lymphoma cell line) whole cell lysate with ab224710 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224710 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: U937 whole cell lysate 10 µg (Input).
Lane 2: ab224710 IP in U937 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224710 in U937 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 20 seconds.
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![Flow Cytometry - Anti-BAFF antibody [EPR22238] (ab224710)](https://www.abcam.com/ps/products/224/ab224710/Images/ab224710-337082-anti-baff-antibody-epr22238-flow-cytometry.jpg)
Flow Cytometry - Anti-BAFF antibody [EPR22238] (ab224710)Flow cytometric analysis of Daudi (human Burkitt's lymphoma cell line; left panel) and HL-60 (human promyelocytic leukemia cell line ;right panel) cell lines labeling BAFF with ab224710 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: Daudi. (PMID: 20951740)
Gated on viable cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR22238] (ab224710)](https://www.abcam.com/ps/products/224/ab224710/Images/ab224710-337081-anti-baff-antibody-epr22238-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR22238] (ab224710)Immunofluorescent analysis of 100% methanol-fixed U937 (human histiocytic lymphoma cell line) cells labeling BAFF with ab224710 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on U937 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Confocal image showing membranous staining in U-937 cells.
We don’t recommend PFA fixation, since the result shows some background staining in the nuclear.
Negative control: HEK-293（PMID: 17393395.
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![Anti-BAFF antibody [EPR22238] (ab224710)](https://www.abcam.com/ps/products/224/ab224710/Images/ab224710-5-benefits-of-recombinant-antibodies.png)
Anti-BAFF antibody [EPR22238] (ab224710)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab224710 has been referenced in 1 publication.
- Liu C et al. microRNA-630 Regulates Underglycosylated IgA1 Production in the Tonsils by Targeting TLR4 in IgA Nephropathy. Front Immunol 11:563699 (2020). PubMed: 33324395