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    products/primary-antibodies/bag2-antibody-epr3567-ab79406.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-BAG2 antibody [EPR3567] (ab79406)

  • Datasheet
  • SDS
Submit a review Submit a question References (5)

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Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAG2 antibody [EPR3567] (ab79406)
  • Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)
  • Immunoprecipitation - Anti-BAG2 antibody [EPR3567] (ab79406)
  • Anti-BAG2 antibody [EPR3567] (ab79406)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3567] to BAG2
  • Suitable for: IP, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-BAG2 antibody [EPR3567]
    See all BAG2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3567] to BAG2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BAG2 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat, HeLa and HepG2 cell lysates. IHC-P: Human ovarian carcinoma tissue. IP: Jurkat lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3567
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human BAG2 knockout HeLa cell line (ab265907)
  • KO cell lysates

    • Human BAG2 knockout HeLa cell lysate (ab257369)
  • Recombinant Protein

    • Recombinant Human BAG2 protein (ab104795)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab79406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
WB
1/1000 - 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
IP
Use at an assay dependent concentration.
WB
1/1000 - 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.
  • Sequence similarities

    Contains 1 BAG domain.
  • Target information above from: UniProt accession O95816 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9532 Human
    • Omim: 603882 Human
    • SwissProt: O95816 Human
    • Unigene: 745046 Human
    • Alternative names

      • BAG 2 antibody
      • BAG family molecular chaperone regulator 2 antibody
      • BAG-2 antibody
      • BAG-family molecular chaperone regulator-2 antibody
      • Bag2 antibody
      • BAG2_HUMAN antibody
      • BCL 2 associated athanogene 2 antibody
      • Bcl-2-associated athanogene 2 antibody
      • BCL2 associated athanogene 2 antibody
      • BCL2-associated athanogene 2 antibody
      • dJ417I1.2 (BAG family molecular chaperone regulator 2) antibody
      • dJ417I1.2 antibody
      • KIAA0576 antibody
      • MGC149462 antibody
      • OTTHUMP00000016668 antibody
      see all

    Images

    • Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)
      Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)
      All lanes : Anti-BAG2 antibody [EPR3567] (ab79406) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : BAG2 knockout HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 24 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?



      Lanes 1-3: Merged signal (red and green). Green - ab79406 observed at 25 kDa. Red - loading control ab8245 observed at 36 kDa. 

       ab79406 Anti-BAG2 antibody [EPR3567] was shown to specifically react with BAG2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265907 (knockout cell lysate ab257369) was used.  Wild-type and BAG2 knockout samples were subjected to SDS-PAGE.  ab79406 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAG2 antibody [EPR3567] (ab79406)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAG2 antibody [EPR3567] (ab79406)

      ab79406, at 1/100 dilution, staining BAG2 in human ovarian carcinoma by Immunohistochemistry using paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)
      Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)
      All lanes : Anti-BAG2 antibody [EPR3567] (ab79406) at 1/5000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 24 kDa
      Observed band size: 24 kDa

    • Immunoprecipitation - Anti-BAG2 antibody [EPR3567] (ab79406)
      Immunoprecipitation - Anti-BAG2 antibody [EPR3567] (ab79406)

      BAG2 was immunoprecipitated from 0.35 mg Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 µg with 79406 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 µg

      Lane 2: ab79406 IP in Jurkat whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab79406 in Jurkat whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Anti-BAG2 antibody [EPR3567] (ab79406)
      Anti-BAG2 antibody [EPR3567] (ab79406)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab79406? Please let us know so that we can cite the reference in this datasheet.

    ab79406 has been referenced in 5 publications.

    • Huang T  et al. Fbxo7 and Pink1 play a reciprocal role in regulating their protein levels. Aging (Albany NY) 13:77-88 (2020). PubMed: 33291077
    • Wang C  et al. Quantitating the epigenetic transformation contributing to cholesterol homeostasis using Gaussian process. Nat Commun 10:5052 (2019). PubMed: 31699992
    • Liu YS & Wei B Over-expression of Bcl2-associated athanogene 2 in oral cancer promotes cellular proliferation and is associated with poor prognosis. Arch Oral Biol 102:164-170 (2019). PubMed: 31055249
    • Swärd K  et al. Identification of the intermediate filament protein synemin/SYNM as a target of myocardin family coactivators. Am J Physiol Cell Physiol 317:C1128-C1142 (2019). PubMed: 31461342
    • Azoitei N  et al. Targeting of KRAS mutant tumors by HSP90 inhibitors involves degradation of STK33. J Exp Med 209:697-711 (2012). WB . PubMed: 22451720

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