Recombinant Anti-BAG2 antibody [EPR3567] (ab79406)
![Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)](https://www.abcam.com/ps/products/79/ab79406/Images/ab79406-393561-anti-bag2-antibody-epr3567-western-blot-human-knockout-lysate.jpg "Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3567] to BAG2
- Suitable for: IP, WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BAG2 antibody [EPR3567]
See all BAG2 primary antibodies -
Description
Rabbit monoclonal [EPR3567] to BAG2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human BAG2 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: Jurkat, HeLa and HepG2 cell lysates. IHC-P: Human ovarian carcinoma tissue. IP: Jurkat lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3567 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB |
1/1000 - 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
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| IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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IP
Use at an assay dependent concentration. |
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WB
1/1000 - 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. -
Sequence similarities
Contains 1 BAG domain. - Information by UniProt
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Database links
- Entrez Gene: 9532 Human
- Omim: 603882 Human
- SwissProt: O95816 Human
- Unigene: 745046 Human
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Alternative names
- BAG 2 antibody
- BAG family molecular chaperone regulator 2 antibody
- BAG-2 antibody
see all
Images
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Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)All lanes : Anti-BAG2 antibody [EPR3567] (ab79406) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BAG2 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab79406 observed at 25 kDa. Red - loading control ab8245 observed at 36 kDa.
ab79406 Anti-BAG2 antibody [EPR3567] was shown to specifically react with BAG2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265907 (knockout cell lysate ab257369) was used. Wild-type and BAG2 knockout samples were subjected to SDS-PAGE. ab79406 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAG2 antibody [EPR3567] (ab79406)](https://www.abcam.com/ps/products/79/ab79406/Images/ab79406-94960-anti-bag2-antibody-epr3567-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAG2 antibody [EPR3567] (ab79406)ab79406, at 1/100 dilution, staining BAG2 in human ovarian carcinoma by Immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)](https://www.abcam.com/ps/products/79/ab79406/Images/ab79406-94961-anti-bag2-antibody-epr3567-western-blot.jpg)
Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)All lanes : Anti-BAG2 antibody [EPR3567] (ab79406) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa -
![Immunoprecipitation - Anti-BAG2 antibody [EPR3567] (ab79406)](https://www.abcam.com/ps/products/79/ab79406/Images/ab79406-5-anti-bag2-antibody-epr3567-immunoprecipitation-jurkat-human.jpg)
Immunoprecipitation - Anti-BAG2 antibody [EPR3567] (ab79406)BAG2 was immunoprecipitated from 0.35 mg Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 µg with 79406 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 µg
Lane 2: ab79406 IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab79406 in Jurkat whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-BAG2 antibody [EPR3567] (ab79406)](https://www.abcam.com/ps/products/79/ab79406/Images/ab79406-4-benefits-of-recombinant-antibodies.png)
Anti-BAG2 antibody [EPR3567] (ab79406)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab79406 has been referenced in 9 publications.
- McDonald EF et al. Distinct proteostasis states drive pharmacologic chaperone susceptibility for cystic fibrosis transmembrane conductance regulator misfolding mutants. Mol Biol Cell 33:ar62 (2022). PubMed: 35389766
- Carrettiero DC et al. Stress routes clients to the proteasome via a BAG2 ubiquitin-independent degradation condensate. Nat Commun 13:3074 (2022). PubMed: 35654899
- Hammond CM et al. DNAJC9 integrates heat shock molecular chaperones into the histone chaperone network. Mol Cell 81:2533-2548.e9 (2021). PubMed: 33857403
- Wang F et al. MicroRNA-325 inhibits the proliferation and induces the apoptosis of T cell acute lymphoblastic leukemia cells in a BAG2-dependent manner. Exp Ther Med 21:631 (2021). PubMed: 33936287
- Huang T et al. Fbxo7 and Pink1 play a reciprocal role in regulating their protein levels. Aging (Albany NY) 13:77-88 (2020). PubMed: 33291077
- Wang C et al. Quantitating the epigenetic transformation contributing to cholesterol homeostasis using Gaussian process. Nat Commun 10:5052 (2019). PubMed: 31699992
- Liu YS & Wei B Over-expression of Bcl2-associated athanogene 2 in oral cancer promotes cellular proliferation and is associated with poor prognosis. Arch Oral Biol 102:164-170 (2019). PubMed: 31055249
- Swärd K et al. Identification of the intermediate filament protein synemin/SYNM as a target of myocardin family coactivators. Am J Physiol Cell Physiol 317:C1128-C1142 (2019). PubMed: 31461342
- Azoitei N et al. Targeting of KRAS mutant tumors by HSP90 inhibitors involves degradation of STK33. J Exp Med 209:697-711 (2012). WB . PubMed: 22451720