Anti-Bag3 antibody (ab47124)
Key features and details
- Rabbit polyclonal to Bag3
- Suitable for: ICC/IF, IP, IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Bag3 antibody
See all Bag3 primary antibodies -
Description
Rabbit polyclonal to Bag3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A recombinant protein fragment corresponding to the C terminal 196 amino acids of human BAG3.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| IHC-P |
1/1000 - 1/5000.
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| IHC-Fr |
Use at an assay dependent concentration.
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| WB | (1) |
1/1000 - 1/2000. Predicted molecular weight: 62 kDa.
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| Notes |
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ICC/IF
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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IHC-P
1/1000 - 1/5000. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
1/1000 - 1/2000. Predicted molecular weight: 62 kDa. |
Target
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Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. -
Involvement in disease
Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material. -
Sequence similarities
Contains 1 BAG domain.
Contains 2 WW domains. - Information by UniProt
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Database links
- Entrez Gene: 9531 Human
- Entrez Gene: 29810 Mouse
- Omim: 603883 Human
- SwissProt: O95817 Human
- SwissProt: Q9JLV1 Mouse
- Unigene: 523309 Human
- Unigene: 84073 Mouse
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Alternative names
- BAG 3 antibody
- BAG family molecular chaperone regulator 3 antibody
- BAG-3 antibody
see all
Images
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Western blot - Anti-Bag3 antibody (ab47124)All lanes : Anti-Bag3 antibody (ab47124) at 1/2000 dilution
Lane 1 : Thymus lysates, total protein (20ug/lane)
Lane 2 : Spleen lysates, total protein (20ug/lane)
Lane 3 : Skeletal(quadriceps) lysates, total protein (20ug/lane)
Lane 4 : Skeletal(diaphragm) lysates, total protein (20ug/lane)
Lane 5 : Smooth (heart) muscle lysates, total protein (20ug/lane)
Predicted band size: 62 kDa
Observed band size: 86 kDa why is the actual band size different from the predicted?
Tissue lysates, normalized for total protein (20 µg/lane), were from a 4 week old male mouse. BAG3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens. -
Immunocytochemistry/ Immunofluorescence - Anti-Bag3 antibody (ab47124)ICC/IF image of ab47124 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47124) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Frozen sections) - Anti-Bag3 antibody (ab47124)Frozen mouse muscle tissue section stained for BAG3 expression using ab47124 at 1/2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins." -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag3 antibody (ab47124)Immunohistochemistry of formalin fixed paraffin embedded human pancreas tissue sections using ab47124 at 1/2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain. -
Western blot - Anti-Bag3 antibody (ab47124)Anti-Bag3 antibody (ab47124) + Hela lysate at 0.5 mg/ml
Predicted band size: 62 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Bag3 antibody (ab47124)Immunocytochemistry/Immunofluorescence of A431 cells stained with Anti-Bag3 antibody (ab47124) green. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Protocols
Datasheets and documents
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Datasheet download
References (21)
ab47124 has been referenced in 21 publications.
- Piccolella M et al. Retinoic Acid Downregulates HSPB8 Gene Expression in Human Breast Cancer Cells MCF-7. Front Oncol 11:652085 (2021). PubMed: 34136389
- Lee S et al. Targeting HSF1 as a Therapeutic Strategy for Multiple Mechanisms of EGFR Inhibitor Resistance in EGFR Mutant Non-Small-Cell Lung Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34203709
- Lu SZ et al. Suppression of astrocytic autophagy by aB-crystallin contributes to a-synuclein inclusion formation. Transl Neurodegener 8:3 (2019). PubMed: 30675347
- Sun H et al. FAM111B, a direct target of p53, promotes the malignant process of lung adenocarcinoma. Onco Targets Ther 12:2829-2842 (2019). PubMed: 31114230
- Al-Tahan S et al. New family with HSPB8-associated autosomal dominant rimmed vacuolar myopathy. Neurol Genet 5:e349 (2019). PubMed: 31403083
