Recombinant Anti-BANK1 antibody [EPR20795] (ab229189)
![Western blot - Anti-BANK1 antibody [EPR20795] (ab229189)](https://www.abcam.com/ps/products/229/ab229189/Images/ab229189-298374-229189WB1.jpg "Western blot - Anti-BANK1 antibody [EPR20795] (ab229189)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20795] to BANK1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-BANK1 antibody [EPR20795]
See all BANK1 primary antibodies -
Description
Rabbit monoclonal [EPR20795] to BANK1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Daudi and Raji whole cell lysates; Human tonsil and fetal spleen lysates. IHC-P: Human tonsil and large B cell lymphoma tissues; Mouse and rat spleen tissues. Flow Cyt (intra): Daudi cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20795 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Buffer
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/600.
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| WB |
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 89 kDa).
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
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Flow Cyt (Intra)
1/600. |
|
WB
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 89 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Relevance
BANK1 is a B cell specific scaffold protein and LYN tyrosine kinase substrate that promotes tyrosine phosphorylation of inositol 1,4,5-trisphosphate receptors. -
Database links
- Entrez Gene: 55024 Human
- Entrez Gene: 242248 Mouse
- Omim: 610292 Human
- SwissProt: Q8NDB2 Human
- SwissProt: Q80VH0 Mouse
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Alternative names
- A530094C12Rik antibody
- AI451642 antibody
- AVIEF antibody
see all
Images
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Western blot - Anti-BANK1 antibody [EPR20795] (ab229189)All lanes : Anti-BANK1 antibody [EPR20795] (ab229189) at 1/1000 dilution
Lane 1 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 89 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with the literature (PMID: 11782428).
The Jurkat cell line is a negative control for BANK1.
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![Western blot - Anti-BANK1 antibody [EPR20795] (ab229189)](https://www.abcam.com/ps/products/229/ab229189/Images/ab229189-298375-229189WB2.jpg)
Western blot - Anti-BANK1 antibody [EPR20795] (ab229189)All lanes : Anti-BANK1 antibody [EPR20795] (ab229189) at 1/1000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : Human fetal spleen lysate
Lane 3 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 89 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Exposure time : Lane 1: 3 minutes; Lane 2: 10 seconds; Lane 3: 58 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 3 was developed on a BIO-RAD® ChemiDoc™ MP instrument.
The molecular mass observed is consistent with the literature (PMID: 11782428).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20795] (ab229189)](https://www.abcam.com/ps/products/229/ab229189/Images/ab229189-298378-229189IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20795] (ab229189)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling BANK1 with ab229189 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining is weak in the germinal center but strong in the mantle zones of human tonsil (PMID: 24879116). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20795] (ab229189)](https://www.abcam.com/ps/products/229/ab229189/Images/ab229189-298379-229189IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20795] (ab229189)Immunohistochemical analysis of paraffin-embedded human large B cell lymphoma tissue labeling BANK1 with ab229189 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in human large B cell lymphoma cells is observed (PMID: 24879116). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20795] (ab229189)](https://www.abcam.com/ps/products/229/ab229189/Images/ab229189-298383-229189IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20795] (ab229189)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling BANK1 with ab229189 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in mouse spleen is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20795] (ab229189)](https://www.abcam.com/ps/products/229/ab229189/Images/ab229189-298386-229189IHC4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20795] (ab229189)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling BANK1 with ab229189 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in rat spleen is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-BANK1 antibody [EPR20795] (ab229189)](https://www.abcam.com/ps/products/229/ab229189/Images/ab229189-298387-229189FC1.jpg)
Flow Cytometry (Intracellular) - Anti-BANK1 antibody [EPR20795] (ab229189)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) (left panel) and Daudi (human Burkitt's lymphoma cell line) (right panel) cell lines labeling BANK1 with ab229189 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor� 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Anti-BANK1 antibody [EPR20795] (ab229189)](https://www.abcam.com/ps/products/229/ab229189/Images/ab229189-8-benefits-of-recombinant-antibodies.png)
Anti-BANK1 antibody [EPR20795] (ab229189)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab229189 has not yet been referenced specifically in any publications.