Anti-Basophils antibody [2D7] (ab155577)
Key features and details
- Mouse monoclonal [2D7] to Basophils
- Suitable for: WB, IHC-P, Flow Cyt, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Basophils antibody [2D7] -
Description
Mouse monoclonal [2D7] to Basophils -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Basophils.
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Positive control
- Chronic myeloid leukaemia (basophilia). Negative control: CD203c negative cells (Basophil depleted cell fractions).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2D7 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
Fresh surgical tissues can be fixed in Carnoy's fluid for 24 h and transferred to absolute ethanol. |
Flow Cyt | (1) |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr | (2) |
Use at an assay dependent concentration.
Fix in either 10% NBF or Carnoy's fluid for 15 minutes at RT. |
ICC/IF | (1) |
Use at an assay dependent concentration.
Cytospin preparations should be fixed in methanol for 15 min, incubated in methanol containing 0.6% H2O2, for 30 min to block endogenous peroxidase. |
Notes |
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WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Fresh surgical tissues can be fixed in Carnoy's fluid for 24 h and transferred to absolute ethanol. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr
Use at an assay dependent concentration. Fix in either 10% NBF or Carnoy's fluid for 15 minutes at RT. |
ICC/IF
Use at an assay dependent concentration. Cytospin preparations should be fixed in methanol for 15 min, incubated in methanol containing 0.6% H2O2, for 30 min to block endogenous peroxidase. |
Target
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Relevance
Basophils are multifunctional haematopoietic cells that secrete a wide variety of proinflammatory agents upon activation such as histamine and cytokines. Moreover, basophils can induce IgE production by B cells. Under normal conditions basophils are primarily produced in the bone marrow, whereas mature basophils reside in the circulation and can enter tissues at sites of inflammation. Both basophils and mast cells have been implicated in the pathogenesis of allergic inflammation due to the abundant expression of high affinity receptors for IgE. The 2D7 antibody can be used as a sensitive and precise marker for human basophils and does not react with lymphocytes, monocytes, eosinophils, neutrophils or mast cells in immunohistochemistry. Moreover, the antibody is suitable for western blot analysis of the 2D7 antigen.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab155577 has been referenced in 6 publications.
- Hashimoto T et al. Pathophysiological mechanisms of itch in bullous pemphigoid. J Am Acad Dermatol N/A:N/A (2019). PubMed: 31351883
- Yanagawa M et al. Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis. J Gastroenterol 53:449-460 (2018). IHC-P ; Human . PubMed: 28921377
- Kim DK et al. MBP-positive and CD11c-positive cells are associated with different phenotypes of Korean patients with non-asthmatic chronic rhinosinusitis. PLoS One 9:e111352 (2014). IHC ; Human . PubMed: 25361058
- Agis H et al. Enumeration and immunohistochemical characterisation of bone marrow basophils in myeloproliferative disorders using the basophil specific monoclonal antibody 2D7. J Clin Pathol 59:396-402 (2006). PubMed: 16461568
- Plager DA et al. Identification of basophils by a mAb directed against pro-major basic protein 1. J Allergy Clin Immunol 117:626-34 (2006). PubMed: 16522463
- Irani AM et al. Immunohistochemical detection of human basophils in late-phase skin reactions. J Allergy Clin Immunol 101:354-62 (1998). PubMed: 9525452