Recombinant Anti-Bax antibody [2D2] (ab270742)
![Western blot - Anti-Bax antibody [2D2] (ab270742)](https://www.abcam.com/ps/products/270/ab270742/Images/ab270742-390009-AntiBax-antibody-2D2-western-blot-mcf7-hela-human.jpg "Western blot - Anti-Bax antibody [2D2] (ab270742)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [2D2] to Bax
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Bax antibody [2D2]
See all Bax primary antibodies -
Description
Mouse monoclonal [2D2] to Bax -
Host species
Mouse -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and MCF7 whole cell lysate. IP: HeLa whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2D2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use a concentration of 15.9 µg/ml.
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| WB |
Use a concentration of 0.477 µg/ml. Predicted molecular weight: 21 kDa.
|
| Notes |
|---|
|
IP
Use a concentration of 15.9 µg/ml. |
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WB
Use a concentration of 0.477 µg/ml. Predicted molecular weight: 21 kDa. |
Target
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Function
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. -
Tissue specificity
Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
Cellular localization
Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. - Information by UniProt
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Database links
- Entrez Gene: 581 Human
- Omim: 600040 Human
- SwissProt: Q07812 Human
- Unigene: 624291 Human
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Alternative names
- Apoptosis regulator BAX antibody
- BAX antibody
- Bax-protein antibody
see all
Images
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Western blot - Anti-Bax antibody [2D2] (ab270742)All lanes : Anti-Bax antibody [2D2] (ab270742) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution
Predicted band size: 21 kDa
Exposure time: 37 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Bax antibody [2D2] (ab270742)](https://www.abcam.com/ps/products/270/ab270742/Images/ab270742-390008-AntiBax-antibody-2D2-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-Bax antibody [2D2] (ab270742)Bax was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate (2μg in 0.35mg lysates) with ab270742 at 15.9µg/ml. Western blot was performed from the immunoprecipitate using ab270742 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 10µg (Input).
Lane 2: ab270742 IP in HeLa whole cell lysate.
Lane 3: Mouse monoclonal IgG instead of ab270742 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 secs. -
![Anti-Bax antibody [2D2] (ab270742)](https://www.abcam.com/ps/products/270/ab270742/Images/ab270742-3-benefits-of-recombinant-antibodies.png)
Anti-Bax antibody [2D2] (ab270742)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab270742 has been referenced in 3 publications.
- Zhang X et al. MicroRNA-365b-3p represses the proliferation and promotes the apoptosis of non-small cell lung cancer cells by targeting PPP5C. Oncol Lett 21:389 (2021). PubMed: 33777212
- Gao Y et al. Surface PEGylated Cancer Cell Membrane-Coated Nanoparticles for Codelivery of Curcumin and Doxorubicin for the Treatment of Multidrug Resistant Esophageal Carcinoma. Front Cell Dev Biol 9:688070 (2021). PubMed: 34386493
- Liu JW et al. Effect of Warm Acupuncture Combined with Bone Marrow Mesenchymal Stem Cells Transplantation on Cartilage Tissue in Rabbit Knee Osteoarthritis. Evid Based Complement Alternat Med 2021:5523726 (2021). PubMed: 34422071