Recombinant Anti-Bax antibody [EPR18283] (ab182733)
![Western blot - Anti-Bax antibody [EPR18283] (ab182733)](https://www.abcam.com/ps/products/182/ab182733/Images/ab182733-275658-anti-bax-antibody-epr18283-western-blot.jpg "Western blot - Anti-Bax antibody [EPR18283] (ab182733)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18283] to Bax
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Bax antibody [EPR18283]
See all Bax primary antibodies -
Description
Rabbit monoclonal [EPR18283] to Bax -
Host species
Rabbit -
Specificity
Expression levels of BAX protein vary with sample type. Induction may be required if endogenous expression is low. -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver, fetal heart, fetal kidney, fetal spleen and fetal lung lysates; Human thymus, cerebellum and lymph node lysates; HeLa, MCF7, HuT-78, Ramos, HEK-293, Raji, L-929, C2C12, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain, kidney and spleen lysates. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18283 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/50.
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| WB | (5) |
1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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| Notes |
|---|
|
IP
1/50. |
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WB
1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
Target
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Function
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. -
Tissue specificity
Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
Cellular localization
Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. - Information by UniProt
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Database links
- Entrez Gene: 581 Human
- Entrez Gene: 12028 Mouse
- Entrez Gene: 24887 Rat
- Omim: 600040 Human
- SwissProt: Q07812 Human
- SwissProt: Q07813 Mouse
- SwissProt: Q63690 Rat
- Unigene: 624291 Human
see all -
Alternative names
- Apoptosis regulator BAX antibody
- BAX antibody
- Bax-protein antibody
see all
Images
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Western blot - Anti-Bax antibody [EPR18283] (ab182733)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: BAX knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HT29 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab182733 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.ab182733 was shown to recognize BAX when BAX knockout samples were used, along with additional cross-reactive bands. Wild-type and BAX knockout samples were subjected to SDS-PAGE. Ab182733 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 2000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Bax antibody [EPR18283] (ab182733)](https://www.abcam.com/ps/products/182/ab182733/Images/ab182733-256208-anti-bax-antibody-epr18283-western-blot.jpg)
Western blot - Anti-Bax antibody [EPR18283] (ab182733)All lanes : Anti-Bax antibody [EPR18283] (ab182733) at 1/2000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal spleen lysate
Lane 5 : Human fetal lung lysate
Lane 6 : Human thymus lysate
Lane 7 : Human cerebellum lysate
Lane 8 : Human lymph node lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2,3 and 4: 8 seconds; Lane 5 and 6: 1 minute; Lane 7 and 8: 15 seconds.
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![Western blot - Anti-Bax antibody [EPR18283] (ab182733)](https://www.abcam.com/ps/products/182/ab182733/Images/ab182733-256206-anti-bax-antibody-epr18283-western-blot.jpg)
Western blot - Anti-Bax antibody [EPR18283] (ab182733)All lanes : Anti-Bax antibody [EPR18283] (ab182733) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HuT-78 (Human Sezary syndrome cell line) whole cell lysate
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 5 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 6 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 7 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 8 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate
Lane 9 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Jurkat doesn't express Bax. (PMID: 9583678).
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![Western blot - Anti-Bax antibody [EPR18283] (ab182733)](https://www.abcam.com/ps/products/182/ab182733/Images/ab182733-256204-anti-bax-antibody-epr18283-western-blot.jpg)
Western blot - Anti-Bax antibody [EPR18283] (ab182733)All lanes : Anti-Bax antibody [EPR18283] (ab182733) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat kidney lysate
Lane 7 : Rat spleen lysate
Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 9 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 10 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 11 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-7: 30 seconds; Lane 8-11: 1 second.
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![Immunoprecipitation - Anti-Bax antibody [EPR18283] (ab182733)](https://www.abcam.com/ps/products/182/ab182733/Images/ab182733-256202-anti-bax-antibody-epr18283-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Bax antibody [EPR18283] (ab182733)Bax was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab182733 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab182733 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate, 10µg (Input).
Lane 2: ab182733 IP in HeLa whole cell lysate.
Lane 3: Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab182733 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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![Western blot - Anti-Bax antibody [EPR18283] (ab182733)](https://www.abcam.com/ps/products/182/ab182733/Images/ab182733-298163-anti-bax-antibody-epr18283-western-blot.jpg)
Western blot - Anti-Bax antibody [EPR18283] (ab182733)All lanes : Anti-Bax antibody [EPR18283] (ab182733) at 1/2000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell). Whole cell lysates
Lane 2 : Hep G2 (Human hepatocellular carcinoma epithelial cell). Whole cell lysates
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) Whole cell lysates
Lane 4 : A549 (Human lung carcinoma epithelial cell) Whole cell lysates
Lane 5 : C2C12 (Mouse myoblasts myoblast) Whole cell lysates
Lane 6 : C6 (Rat glial tumor glial cell) Whole cell lysates
Lane 7 : Mouse brain. Whole tissue lysate
Lane 8 : Rat brain. Whole tissue lysate
Lane 9 : Rat Spleen. Whole tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 21 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking and Diluting buffers: 5% NFDM/TBST
Exposure time 58 s
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![Anti-Bax antibody [EPR18283] (ab182733)](https://www.abcam.com/ps/products/182/ab182733/Images/ab182733-7-benefits-of-recombinant-antibodies.png)
Anti-Bax antibody [EPR18283] (ab182733)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (137)
ab182733 has been referenced in 137 publications.
- Pan X et al. METTL3 promotes adriamycin resistance in MCF-7 breast cancer cells by accelerating pri-microRNA-221-3p maturation in a m6A-dependent manner. Exp Mol Med N/A:N/A (2021). PubMed: 33420414
- Liu C et al. Cytoplasmic SHMT2 drives the progression and metastasis of colorectal cancer by inhibiting ß-catenin degradation. Theranostics 11:2966-2986 (2021). PubMed: 33456583
- Lv R et al. Z-Guggulsterone Induces Apoptosis in Gastric Cancer Cells through the Intrinsic Mitochondria-Dependent Pathway. ScientificWorldJournal 2021:3152304 (2021). PubMed: 33488300
- Xie F et al. Long non-coding RNA LINC00488 facilitates thyroid cancer cell progression through miR-376a-3p/PON2. Biosci Rep 41:N/A (2021). PubMed: 33600548
- Li Y et al. microRNA-130a-5p suppresses myocardial ischemia reperfusion injury by downregulating the HMGB2/NF-?B axis. BMC Cardiovasc Disord 21:121 (2021). PubMed: 33658008