Anti-BCKDHA (phospho S293) antibody (ab200577)
Key features and details
- Rabbit polyclonal to BCKDHA (phospho S293)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BCKDHA (phospho S293) antibody
See all BCKDHA primary antibodies -
Description
Rabbit polyclonal to BCKDHA (phospho S293) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within BCKDHA (phospho S293). The exact sequence is proprietary.
Database link: P12694 -
Positive control
- WB: NIH 3T3, CT26, CH27, TCMK-1, BW5147.3, Hep-G2, HeLa, and LNCaP Whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/10000 - 1/25000. Predicted molecular weight: 50 kDa.
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| Notes |
|---|
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WB
1/10000 - 1/25000. Predicted molecular weight: 50 kDa. |
Target
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Function
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). -
Involvement in disease
Maple syrup urine disease 1A -
Sequence similarities
Belongs to the BCKDHA family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 468889 Chimpanzee
- Entrez Gene: 282149 Cow
- Entrez Gene: 484488 Dog
- Entrez Gene: 101128832 Gorilla
- Entrez Gene: 593 Human
- Entrez Gene: 12039 Mouse
- Entrez Gene: 100462553 Orangutan
- Entrez Gene: 100142666 Pig
see all -
Alternative names
- Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
- FLJ45695 antibody
- OVD1A antibody
see all
Images
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Western blot - Anti-BCKDHA (phospho S293) antibody (ab200577)All lanes : Anti-BCKDHA (phospho S293) antibody (ab200577) at 1/25000 dilution
Lane 1 : Hep-G2 Whole cell lysate
Lane 2 : HeLa Whole cell lysate
Lane 3 : LNCaP Whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDaDetection: Chemiluminescence with an exposure time of 3 minutes.
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Western blot - Anti-BCKDHA (phospho S293) antibody (ab200577)All lanes : Anti-BCKDHA (phospho S293) antibody (ab200577) at 1/25000 dilution
Lane 1 : NIH 3T3 Whole cell lysate
Lane 2 : CT26 Whole cell lysate
Lane 3 : CH27 Whole cell lysate
Lane 4 : TCMK-1 Whole cell lysate
Lane 5 : BW5147.3 Whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDaDetection: Chemiluminescence with an exposure time of 3 minutes.
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab200577 has been referenced in 14 publications.
- Wu G et al. Exercise Enhances Branched-Chain Amino Acid Catabolism and Decreases Cardiac Vulnerability to Myocardial Ischemic Injury. Cells 11:N/A (2022). PubMed: 35626742
- Pontoizeau C et al. Neonatal gene therapy achieves sustained disease rescue of maple syrup urine disease in mice. Nat Commun 13:3278 (2022). PubMed: 35672312
- Lee J et al. BCAA Supplementation in Mice with Diet-induced Obesity Alters the Metabolome Without Impairing Glucose Homeostasis. Endocrinology 162:N/A (2021). PubMed: 33765118
- Yoshida N et al. Bacteroides spp. promotes branched-chain amino acid catabolism in brown fat and inhibits obesity. iScience 24:103342 (2021). PubMed: 34805797
- Kaya E et al. Beneficial Effects of Acetyl-DL-Leucine (ADLL) in a Mouse Model of Sandhoff Disease. J Clin Med 9:N/A (2020). PubMed: 32276303