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  1. Link

    products/primary-antibodies/bcl10-antibody-4f8e8h8-ab36585.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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Anti-Bcl10 antibody [4F8E8H8] (ab36585)

  • Datasheet
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Western blot - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
  • Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
  • Flow Cytometry - Anti-Bcl10 antibody [4F8E8H8] (ab36585)

Key features and details

  • Mouse monoclonal [4F8E8H8] to Bcl10
  • Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Bcl10 antibody [4F8E8H8]
    See all Bcl10 primary antibodies
  • Description

    Mouse monoclonal [4F8E8H8] to Bcl10
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3).

  • Positive control

    • MCF-7 cell extract. Human breast and liver carcinoma tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    4F8E8H8
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • CARD Family
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Bcl10 protein (ab124575)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab36585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
WB
1/500 - 1/2000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
Flow Cyt
1/200.
ICC/IF
1/200 - 1/1000.
Notes
IHC-P
Use at an assay dependent concentration.
WB
1/500 - 1/2000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
Flow Cyt
1/200.
ICC/IF
1/200 - 1/1000.

Target

  • Function

    Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
    Note=Defects in BCL10 are involved in various types of cancer.
  • Sequence similarities

    Contains 1 CARD domain.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
  • Cellular localization

    Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
  • Target information above from: UniProt accession O95999 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8915 Human
    • Entrez Gene: 12042 Mouse
    • Omim: 603517 Human
    • SwissProt: O95999 Human
    • SwissProt: Q9Z0H7 Mouse
    • Unigene: 193516 Human
    • Unigene: 239141 Mouse
    • Alternative names

      • AI132454 antibody
      • B cell CLL/lymphoma 10 antibody
      • B cell lymphoma/leukemia10 antibody
      • B-cell CLL/lymphoma 10 antibody
      • B-cell leukemia/lymphoma 10 antibody
      • B-cell lymphoma/leukemia 10 antibody
      • Bcl 10 antibody
      • Bcl-10 antibody
      • Bcl10 antibody
      • BCL10_HUMAN antibody
      • c E10 antibody
      • c-E10 antibody
      • C81403 antibody
      • CARD containing apoptotic signaling protein antibody
      • CARD containing molecule enhancing NF kappa B antibody
      • CARD containing molecule enhancing NF kB antibody
      • CARD containing molecule enhancing NF-kB antibody
      • CARD containing molecule enhancing NFkB antibody
      • CARD containing proapoptotic protein antibody
      • CARD like apoptotic protein antibody
      • CARD-containing apoptotic signaling protein antibody
      • CARD-containing molecule enhancing NF-kappa-B antibody
      • CARD-containing proapoptotic protein antibody
      • CARD-like apoptotic protein antibody
      • CARMEN antibody
      • Caspase recruiting domain containing protein antibody
      • caspase-recruiting domain-containing protein antibody
      • cCARMEN antibody
      • cE 10 antibody
      • cE10 antibody
      • CED 3/ICH 1 prodomain homologous E10 like regulator antibody
      • CED-3/ICH-1 prodomain homologous E10-like regulator antibody
      • CED3/ICH1 prodomain homologous E10 like regulator antibody
      • Cellular E10 antibody
      • Cellular homolog of vCARMEN antibody
      • Cellular-E10 antibody
      • CIPER antibody
      • CLAP antibody
      • hCLAP antibody
      • Mammalian CARD containing adapter molecule E10 antibody
      • Mammalian CARD-containing adapter molecule E10 antibody
      • mE 10 antibody
      • mE10 antibody
      • R-RCD1 antibody
      see all

    Images

    • Western blot - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
      Western blot - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
      All lanes : Anti-Bcl10 antibody [4F8E8H8] (ab36585) at 1/500 dilution

      Lane 1 : Cell lysates prepared from NIH/3T3 cells
      Lane 2 : Cell lysates prepared from Hela cells
      Lane 3 : Cell lysates prepared from MCF-7 cells
      Lane 4 : Cell lysates prepared from Jurkat cells

      Lysates/proteins at 100 µg per lane.

      Secondary
      All lanes : HRP-conjugated Goat polyclonal to mouse IgG1

      Predicted band size: 31 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
      Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human liver carcinoma tissue by immunohistochemistry with DAB staining.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
      Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human breast carcinoma tissue by immunohistochemistry with DAB staining.
    • Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
      Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody [4F8E8H8] (ab36585)

      ab36585 at 1/1000 dilution staining Bcl10 in human Hela cells by Immunocytochemistry/ Immunofluorescence. Bcl10 show green staining in image and actin filament stained red with Alexa Fluor®555 conjugated secondary. Nuclei stained blue with DRAQ5 fluorescent DNA dye.

    • Flow Cytometry - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
      Flow Cytometry - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
      Flow cytometric analysis of Hela cells using ab36585 at a 1/200 dilution (green) and negative control (purple).

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab36585? Please let us know so that we can cite the reference in this datasheet.

    ab36585 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I'd like to get more information about your antibody, anti-Bcl10 (product #ab36585), especially epitope region.

    Does your Bcl10 antibody detect N-terminal or C-terminal?

    I'm looking for Bcl10 western blot antibody, which detect C-terminal region, not CARD domain in N-terminal, and reactive in mouse.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 03 2012

    Answer

    Thank you for contacting us.
    The antibody ab36585 was raised agianst the Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3) - thus we do not know where the epitope region is and whether it is N- or C-terminal.
    This is the only antibody that has been tested in mouse. Other antibodies were tested in WB, but not mouse or do not react with mouse.

    We have ab93604, ab128183, and ab1142 that were raised against a C-terminal region of Bcl10. If you like to try any of these for mouse samples, you can use our Abtrial program.

    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
    The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the to-be-tested product at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount in the amount of the price you paid for the to-be-tested product. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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