Anti-Bcl10 antibody (ab227206)
Key features and details
- Rabbit polyclonal to Bcl10
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Bcl10 antibody
See all Bcl10 primary antibodies -
Description
Rabbit polyclonal to Bcl10 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human Bcl10 (internal sequence). The exact sequence is proprietary.
Database link: O95999 -
Positive control
- WB: HeLa cell extract; Raji, Neuro-2a, GL261 and C8D30 whole cell lysates. IP: Raji whole cell extract. IHC-P: Human gastric cancer tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| IP |
1/100 - 1/500.
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| Notes |
|---|
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IHC-P
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
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ICC/IF
1/100 - 1/1000. |
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IP
1/100 - 1/500. |
Target
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Function
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer. -
Sequence similarities
Contains 1 CARD domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. -
Cellular localization
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 540824 Cow
- Entrez Gene: 8915 Human
- Entrez Gene: 12042 Mouse
- Entrez Gene: 100125346 Pig
- Entrez Gene: 83477 Rat
- Entrez Gene: 714869 Rhesus monkey
- Omim: 603517 Human
- SwissProt: O95999 Human
see all -
Alternative names
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
Images
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Western blot - Anti-Bcl10 antibody (ab227206)All lanes : Anti-Bcl10 antibody (ab227206) at 1/2500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Bcl10 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 26 kDa -
Western blot - Anti-Bcl10 antibody (ab227206)All lanes : Anti-Bcl10 antibody (ab227206) at 1/2500 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : Bcl10 knockout HeLa cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 26 kDa12% SDS-PAGE gel.
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Western blot - Anti-Bcl10 antibody (ab227206)Anti-Bcl10 antibody (ab227206) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 26 kDa12% SDS PAGE gel.
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Western blot - Anti-Bcl10 antibody (ab227206)All lanes : Anti-Bcl10 antibody (ab227206) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa12 % SDS-PAGE gel.
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Immunoprecipitation - Anti-Bcl10 antibody (ab227206)Bcl10 was immunoprecipitated from Raji (human Burkitt's lymphoma cell line) whole cell extract with 4 μg ab227206. Western blot was performed from the immunoprecipitate using ab227206 at 1/500 dilution. Anti-rabbit IgG was used as a secondary reagent
Lane 1: Raji whole cell extract 40 μg (Input).
Lane 2: Control IP in Raji whole cell extract with 4 μg of preimmune Rabbit IgG.
Lane 3: ab227206 IP in Raji whole cell extract.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody (ab227206)Paraffin-embedded human gastric cancer tissue stained for Bcl10 using ab227206 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody (ab227206)2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Bcl10 (green) using ab227206 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227206 has not yet been referenced specifically in any publications.