Recombinant Anti-Bcl10 antibody [ep605y] (ab40752)
![Immunoprecipitation - Anti-Bcl10 antibody [ep605y] (ab40752)](https://www.abcam.com/ps/products/40/ab40752/Images/ab40752-501391-anti-bcl10-antibody-ep605y-immunoprecipitation-mouse.jpg "Immunoprecipitation - Anti-Bcl10 antibody [ep605y] (ab40752)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [ep605y] to Bcl10
- Suitable for: Flow Cyt (Intra), WB, IHC-P, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Bcl10 antibody [ep605y]
See all Bcl10 primary antibodies -
Description
Rabbit monoclonal [ep605y] to Bcl10 -
Host species
Rabbit -
Specificity
Not suitable for mouse IHC-P.
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Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Bcl10 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: Raji, WEHI-231, RAW 264.7 whole cell and mouse spleen tissue lysates. Flow Cyt (intra): Daudi cells. IP: Daudi and WEHI-231 whole cell lystates. IHC-P: Human colon carcinoma.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
ep605y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
1/30 - 1/50.
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| Notes |
|---|
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
1/30 - 1/50. |
Target
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Function
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer. -
Sequence similarities
Contains 1 CARD domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. -
Cellular localization
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 8915 Human
- Entrez Gene: 12042 Mouse
- Omim: 603517 Human
- SwissProt: O95999 Human
- SwissProt: Q9Z0H7 Mouse
- Unigene: 193516 Human
- Unigene: 239141 Mouse
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Alternative names
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
Images
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Immunoprecipitation - Anti-Bcl10 antibody [ep605y] (ab40752)
32 kDa Bcl10 was immunoprecipitated from 0.35mg WEHI-231 (mouse B cell lymphoma B lymphocyte ), whole cell lysate with ab40752 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab40752 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1 (Input): WEHI-231 (mouse B cell lymphoma B lymphocyte ), whole cell lysate 10 μg.
Lane 2 (+): WEHI-231 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40752 in WEHI-231 whole cell lysate.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 102 seconds.
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![Immunoprecipitation - Anti-Bcl10 antibody [ep605y] (ab40752)](https://www.abcam.com/ps/products/40/ab40752/Images/ab40752-501390-anti-bcl10-antibody-ep605y-immunoprecipitation-human.jpg)
Immunoprecipitation - Anti-Bcl10 antibody [ep605y] (ab40752)32 kDa Bcl10 was immunoprecipitated from 0.35mg of Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate with ab40752 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab40752 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1 (Input): Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate 10 μg.
Lane 2 (+): Daudi whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40752 in Daudi whole cell lysate.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds.
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![Western blot - Anti-Bcl10 antibody [ep605y] (ab40752)](https://www.abcam.com/ps/products/40/ab40752/Images/ab40752-501388-anti-bcl10-antibody-ep605y-western-blot-mouse.jpg)
Western blot - Anti-Bcl10 antibody [ep605y] (ab40752)All lanes : Anti-Bcl10 antibody [ep605y] (ab40752) at 1/2000 dilution
Lane 1 : WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate
Lane 2 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lane 5 : Mouse bone marrow lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Exposure time: 103 secondsBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
ab129002 was used as a loading control.
The expression profile observed is consistent with what has been described in the literature (PMID: 10319863).
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![Western blot - Anti-Bcl10 antibody [ep605y] (ab40752)](https://www.abcam.com/ps/products/40/ab40752/Images/ab40752-53851-anti-bcl10-antibody-ep605y-western-blot.jpg)
Western blot - Anti-Bcl10 antibody [ep605y] (ab40752)Anti-Bcl10 antibody [ep605y] (ab40752) at 1/2000 dilution + Raji cell lysate at 10 µg
Predicted band size: 31 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [ep605y] (ab40752)](https://www.abcam.com/ps/products/40/ab40752/Images/ab40752-53850-anti-bcl10-antibody-ep605y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [ep605y] (ab40752)Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ab40752 at a dilution of 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-Bcl10 antibody [ep605y] (ab40752)](https://www.abcam.com/ps/products/40/ab40752/Images/ab40752-4-anti-bcl10-antibody-ep605y-flowcytometry-daudi-human.jpg)
Flow Cytometry (Intracellular) - Anti-Bcl10 antibody [ep605y] (ab40752)Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling Bcl10 with purified ab40752 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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![Immunoprecipitation - Anti-Bcl10 antibody [ep605y] (ab40752)](https://www.abcam.com/ps/products/40/ab40752/Images/ab40752-5-anti-bcl10-antibody-ep605y-immunoprecipitation-daudi-human.jpg)
Immunoprecipitation - Anti-Bcl10 antibody [ep605y] (ab40752)Purified ab40752 at 1/50 dilution (2µg) immunoprecipitating Bcl10 in Daudi whole cell lysate.
Lane 1 (input): Daudi (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab40752 + Daudi whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40752 in Daudi whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 32 kDa -
![Anti-Bcl10 antibody [ep605y] (ab40752)](https://www.abcam.com/ps/products/40/ab40752/Images/ab40752-3-benefits-of-recombinant-antibodies.png)
Anti-Bcl10 antibody [ep605y] (ab40752)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab40752 has been referenced in 1 publication.
- Ginster S et al. Two Antagonistic MALT1 Auto-Cleavage Mechanisms Reveal a Role for TRAF6 to Unleash MALT1 Activation. PLoS One 12:e0169026 (2017). WB . PubMed: 28052131