Recombinant Anti-Bcl10 antibody [EPR26560-101] (ab302619)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26560-101] to Bcl10
- Suitable for: WB, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Bcl10 antibody [EPR26560-101]
See all Bcl10 primary antibodies -
Description
Rabbit monoclonal [EPR26560-101] to Bcl10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse
Does not react with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse spleen and skeletal muscle tissue lysates; A20 and NIH/3T3 whole cell lysates. IP: A20 whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26560-101 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab302619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 26 kDa).
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IP |
1/30.
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Notes |
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WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 26 kDa). |
IP
1/30. |
Target
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Function
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer. -
Sequence similarities
Contains 1 CARD domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. -
Cellular localization
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 12042 Mouse
- SwissProt: Q9Z0H7 Mouse
- Unigene: 239141 Mouse
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Alternative names
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
Images
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All lanes : Anti-Bcl10 antibody [EPR26560-101] (ab302619) at 1/1000 dilution
Lane 1 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking / Diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
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All lanes : Anti-Bcl10 antibody [EPR26560-101] (ab302619) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 125 secondsBlocking / Diluting buffer and concentration: 5% NFDM/TBST
Low expression: skeletal muscle (PMID: 10319863)
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Bcl10 was immunoprecipitated from 0.35 mg A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate 10 µg with ab302619 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302619 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate 10 µg
Lane 2: ab302619 IP in A20 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302619 in A20 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
Observed MW(KDa): 28
The lysate was freshly made and used for IP immediately to minimize protein degradation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab302619 has not yet been referenced specifically in any publications.