Recombinant Anti-Bcl10 antibody [EPR26560-101] (ab302619)
![Western blot - Anti-Bcl10 antibody [EPR26560-101] (AB302619)](https://www.abcam.com/ps/products/302/ab302619/Images/AB302619-489481-anti-bcl10antibody-epr26560101-western-blot-a20.jpg "Western blot - Anti-Bcl10 antibody [EPR26560-101] (AB302619)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26560-101] to Bcl10
- Suitable for: WB, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Bcl10 antibody [EPR26560-101]
See all Bcl10 primary antibodies -
Description
Rabbit monoclonal [EPR26560-101] to Bcl10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse
Does not react with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse spleen and skeletal muscle tissue lysates; A20 and NIH/3T3 whole cell lysates. IP: A20 whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26560-101 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab302619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 26 kDa).
|
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| IP |
1/30.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 26 kDa). |
|
IP
1/30. |
Target
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Function
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer. -
Sequence similarities
Contains 1 CARD domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. -
Cellular localization
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 12042 Mouse
- SwissProt: Q9Z0H7 Mouse
- Unigene: 239141 Mouse
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Alternative names
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
Images
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Western blot - Anti-Bcl10 antibody [EPR26560-101] (AB302619)All lanes : Anti-Bcl10 antibody [EPR26560-101] (ab302619) at 1/1000 dilution
Lane 1 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking / Diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
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![Western blot - Anti-Bcl10 antibody [EPR26560-101] (AB302619)](https://www.abcam.com/ps/products/302/ab302619/Images/AB302619-489482-anti-bcl10antibody-epr26560101-western-blot-mouse-spleen.jpg)
Western blot - Anti-Bcl10 antibody [EPR26560-101] (AB302619)All lanes : Anti-Bcl10 antibody [EPR26560-101] (ab302619) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 125 secondsBlocking / Diluting buffer and concentration: 5% NFDM/TBST
Low expression: skeletal muscle (PMID: 10319863)
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![Immunoprecipitation - Anti-Bcl10 antibody [EPR26560-101] (AB302619)](https://www.abcam.com/ps/products/302/ab302619/Images/AB302619-2-anti-bcl10antibody-epr26560101-immunoprecipitation-a20.jpg)
Immunoprecipitation - Anti-Bcl10 antibody [EPR26560-101] (AB302619)Bcl10 was immunoprecipitated from 0.35 mg A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate 10 µg with ab302619 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302619 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate 10 µg
Lane 2: ab302619 IP in A20 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302619 in A20 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
Observed MW(KDa): 28
The lysate was freshly made and used for IP immediately to minimize protein degradation.
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![Anti-Bcl10 antibody [EPR26560-101] (AB302619)](https://www.abcam.com/ps/products/302/ab302619/Images/AB302619-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-Bcl10 antibody [EPR26560-101] (AB302619)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab302619 has not yet been referenced specifically in any publications.