Recombinant Anti-Bcl10 antibody [EPR26560-101] - BSA and Azide free (ab302620)
![Western blot - Anti-Bcl10 antibody [EPR26560-101] (BSA and Azide free) (AB302620)](https://www.abcam.com/ps/products/302/ab302620/Images/AB302620-489483-anti-bcl10antibody-epr26560101-western-blot-a20.jpg "Western blot - Anti-Bcl10 antibody [EPR26560-101] (BSA and Azide free) (AB302620)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26560-101] to Bcl10 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Bcl10 antibody [EPR26560-101] - BSA and Azide free
See all Bcl10 primary antibodies -
Description
Rabbit monoclonal [EPR26560-101] to Bcl10 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse
Does not react with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse spleen and skeletal muscle tissue lysates; A20 and NIH/3T3 whole cell lysates. IP: A20 whole cell lysate.
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General notes
ab302620 is the carrier-free version of ab302619.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26560-101 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab302620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 26 kDa).
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 26 kDa). |
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IP
Use at an assay dependent concentration. |
Target
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Function
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer. -
Sequence similarities
Contains 1 CARD domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. -
Cellular localization
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 12042 Mouse
- SwissProt: Q9Z0H7 Mouse
- Unigene: 239141 Mouse
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Alternative names
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
Images
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Western blot - Anti-Bcl10 antibody [EPR26560-101] (BSA and Azide free) (AB302620)All lanes : Anti-Bcl10 antibody [EPR26560-101] (ab302619) at 1/1000 dilution
Lane 1 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab302619, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
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![Western blot - Anti-Bcl10 antibody [EPR26560-101] (BSA and Azide free) (AB302620)](https://www.abcam.com/ps/products/302/ab302620/Images/AB302620-489484-anti-bcl10antibody-epr26560101-western-blot-mouse-spleen.jpg)
Western blot - Anti-Bcl10 antibody [EPR26560-101] (BSA and Azide free) (AB302620)All lanes : Anti-Bcl10 antibody [EPR26560-101] (ab302619) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 125 secondsThis data was developed using ab302619, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer and concentration: 5% NFDM/TBST
Low expression: skeletal muscle (PMID: 10319863)
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![Immunoprecipitation - Anti-Bcl10 antibody [EPR26560-101] (BSA and Azide free) (AB302620)](https://www.abcam.com/ps/products/302/ab302620/Images/AB302620-2-anti-bcl10antibody-epr26560101-immunoprecipitation-a20.jpg)
Immunoprecipitation - Anti-Bcl10 antibody [EPR26560-101] (BSA and Azide free) (AB302620)This data was developed using ab302619, the same antibody clone in a different buffer formulation.
Bcl10 was immunoprecipitated from 0.35 mg A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate 10 µg with ab302619 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302619 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate 10 µg
Lane 2: ab302619 IP in A20 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302619 in A20 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
Observed MW(KDa): 28
The lysate was freshly made and used for IP immediately to minimize protein degradation.
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![Anti-Bcl10 antibody [EPR26560-101] (BSA and Azide free) (AB302620)](https://www.abcam.com/ps/products/302/ab302620/Images/AB302620-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-Bcl10 antibody [EPR26560-101] (BSA and Azide free) (AB302620)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab302620 has not yet been referenced specifically in any publications.
