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  1. Link

    products/primary-antibodies/bcl10-antibody-epr8587-ab150380.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Bcl10 antibody [EPR8587] (ab150380)

  • Datasheet
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Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)
  • Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)
  • Anti-Bcl10 antibody [EPR8587] (ab150380)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8587] to Bcl10
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human Bcl10 protein (ab124575)
Knockout
Product image
Human BCL10 knockout HeLa cell line (ab261797)

View more associated products

Overview

  • Product name

    Anti-Bcl10 antibody [EPR8587]
    See all Bcl10 primary antibodies
  • Description

    Rabbit monoclonal [EPR8587] to Bcl10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Bcl10 aa 1-250.

  • Positive control

    • WB: Recombinant Human Bcl10 protein (ab124575); Ramos, HeLa, HuT-78 and Raji cell lysates. IHC-P: Human MALT and tonsil tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8587
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • CARD Family
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-Bcl10 antibody [EPR8587] - BSA and Azide free (ab248953)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human BCL10 knockout HeLa cell line (ab261797)
  • KO cell lysates

    • Human BCL10 knockout HeLa cell lysate (ab257144)
  • Positive Controls

    • Recombinant Human Bcl10 protein (ab124575)
    • HeLa whole cell lysate (ab29545)
    • Ramos whole cell lysate (ab3955)
  • Recombinant Protein

    • Recombinant Human Bcl10 protein (ab124575)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
    Note=Defects in BCL10 are involved in various types of cancer.
  • Sequence similarities

    Contains 1 CARD domain.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
  • Cellular localization

    Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
  • Target information above from: UniProt accession O95999 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8915 Human
    • Omim: 603517 Human
    • SwissProt: O95999 Human
    • Unigene: 193516 Human
    • Alternative names

      • AI132454 antibody
      • B cell CLL/lymphoma 10 antibody
      • B cell lymphoma/leukemia10 antibody
      • B-cell CLL/lymphoma 10 antibody
      • B-cell leukemia/lymphoma 10 antibody
      • B-cell lymphoma/leukemia 10 antibody
      • Bcl 10 antibody
      • Bcl-10 antibody
      • Bcl10 antibody
      • BCL10_HUMAN antibody
      • c E10 antibody
      • c-E10 antibody
      • C81403 antibody
      • CARD containing apoptotic signaling protein antibody
      • CARD containing molecule enhancing NF kappa B antibody
      • CARD containing molecule enhancing NF kB antibody
      • CARD containing molecule enhancing NF-kB antibody
      • CARD containing molecule enhancing NFkB antibody
      • CARD containing proapoptotic protein antibody
      • CARD like apoptotic protein antibody
      • CARD-containing apoptotic signaling protein antibody
      • CARD-containing molecule enhancing NF-kappa-B antibody
      • CARD-containing proapoptotic protein antibody
      • CARD-like apoptotic protein antibody
      • CARMEN antibody
      • Caspase recruiting domain containing protein antibody
      • caspase-recruiting domain-containing protein antibody
      • cCARMEN antibody
      • cE 10 antibody
      • cE10 antibody
      • CED 3/ICH 1 prodomain homologous E10 like regulator antibody
      • CED-3/ICH-1 prodomain homologous E10-like regulator antibody
      • CED3/ICH1 prodomain homologous E10 like regulator antibody
      • Cellular E10 antibody
      • Cellular homolog of vCARMEN antibody
      • Cellular-E10 antibody
      • CIPER antibody
      • CLAP antibody
      • hCLAP antibody
      • Mammalian CARD containing adapter molecule E10 antibody
      • Mammalian CARD-containing adapter molecule E10 antibody
      • mE 10 antibody
      • mE10 antibody
      • R-RCD1 antibody
      see all

    Images

    • Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)
      Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)
      All lanes : Anti-Bcl10 antibody [EPR8587] (ab150380) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : BCL10 knockout HeLa cell lysate
      Lane 3 : Romas cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 26 kDa
      Observed band size: 32 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab150380 observed at 32 kDa. Red - loading control, ab7291 observed at 52 kDa.

      ab150380 Anti-Bcl10 antibody [EPR8587] was shown to specifically react with Bcl10 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261797 (knockout cell lysate ab257144) was used. Wild-type and Bcl10 knockout samples were subjected to SDS-PAGE. ab150380 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)
      Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)
      All lanes : Anti-Bcl10 antibody [EPR8587] (ab150380) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HuT-78 cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size: 26 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)

      Immunohistochemical analysis of paraffin embedded Human MALT tissue labeling Bcl10 with ab150380 at a 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)

      Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Bcl10 with ab150380 at a 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Bcl10 antibody [EPR8587] (ab150380)
      Anti-Bcl10 antibody [EPR8587] (ab150380)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab150380? Please let us know so that we can cite the reference in this datasheet.

    ab150380 has not yet been referenced specifically in any publications.

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