Recombinant Anti-Bcl10 antibody [EPR8587] (ab150380)
![Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)](https://www.abcam.com/ps/products/150/ab150380/Images/ab150380-384458-anti-bcl10-antibody-epr8587-western-blot-hela-ramos-a549.jpg "Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8587] to Bcl10
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Bcl10 antibody [EPR8587]
See all Bcl10 primary antibodies -
Description
Rabbit monoclonal [EPR8587] to Bcl10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Bcl10 aa 1-250.
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Positive control
- WB: Recombinant Human Bcl10 protein (ab124575); Ramos, HeLa, HuT-78 and Raji cell lysates. IHC-P: Human MALT and tonsil tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8587 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150380 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer. -
Sequence similarities
Contains 1 CARD domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. -
Cellular localization
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 8915 Human
- Omim: 603517 Human
- SwissProt: O95999 Human
- Unigene: 193516 Human
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Alternative names
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
Images
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Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)All lanes : Anti-Bcl10 antibody [EPR8587] (ab150380) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BCL10 knockout HeLa cell lysate
Lane 3 : Romas cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab150380 observed at 32 kDa. Red - loading control, ab7291 observed at 52 kDa.
ab150380 Anti-Bcl10 antibody [EPR8587] was shown to specifically react with Bcl10 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261797 (knockout cell lysate ab257144) was used. Wild-type and Bcl10 knockout samples were subjected to SDS-PAGE. ab150380 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)](https://www.abcam.com/ps/products/150/ab150380/Images/ab150380-156969-anti-bcl10-antibody-epr8587-western-blot.jpg)
Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)All lanes : Anti-Bcl10 antibody [EPR8587] (ab150380) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HuT-78 cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 26 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)](https://www.abcam.com/ps/products/150/ab150380/Images/ab150380-156968-anti-bcl10-antibody-epr8587-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)Immunohistochemical analysis of paraffin embedded Human MALT tissue labeling Bcl10 with ab150380 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)](https://www.abcam.com/ps/products/150/ab150380/Images/ab150380-156967-anti-bcl10-antibody-epr8587-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Bcl10 with ab150380 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Bcl10 antibody [EPR8587] (ab150380)](https://www.abcam.com/ps/products/150/ab150380/Images/ab150380-5-benefits-of-recombinant-antibodies.png)
Anti-Bcl10 antibody [EPR8587] (ab150380)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab150380 has not yet been referenced specifically in any publications.