Anti-BCL2L10 antibody [8A2F9] (ab233641)
Key features and details
- Mouse monoclonal [8A2F9] to BCL2L10
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-BCL2L10 antibody [8A2F9] -
Description
Mouse monoclonal [8A2F9] to BCL2L10 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human BCL2L10 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: Q9HD36 -
Positive control
- WB: BCL2L10 (aa 31-186)-hIgGFc transfected HEK-293 cell lysate; Human BCL2L10 (aa 31-186) recombinant protein. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IHC-P: Human lung cancer and cervical cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
8A2F9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 22 kDa.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
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ICC/IF |
1/200 - 1/1000.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 22 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. |
ICC/IF
1/200 - 1/1000. |
Target
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Function
Promotes cell survival. Suppresses apoptosis induced by BAX but not BAK. -
Tissue specificity
Widely expressed in adult tissues. Preferentially expressed in lung, liver and kidney. -
Sequence similarities
Belongs to the Bcl-2 family. -
Cellular localization
Mitochondrion. Nucleus membrane. - Information by UniProt
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Database links
- Entrez Gene: 10017 Human
- Omim: 606910 Human
- SwissProt: Q9HD36 Human
- Unigene: 283672 Human
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Alternative names
- Anti apoptotic protein NrH antibody
- Anti-apoptotic protein NrH antibody
- Apoptosis regulator Bcl B antibody
see all
Images
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Anti-BCL2L10 antibody [8A2F9] (ab233641) at 1/500 dilution + Human BCL2L10 (aa 31-186) recombinant protein
Predicted band size: 22 kDaExpected MW is 43.1 kDa.
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All lanes : Anti-BCL2L10 antibody [8A2F9] (ab233641) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : BCL2L10 (aa 31-186)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 22 kDa -
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for BCL2L10 (green) using ab233641 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling BCL2L10 with ab233641 at 1/200 dilution (green) compared with a negative control (red).
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Paraffin-embedded human lung cancer tissue stained for BCL2L10 using ab233641 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human cervical cancer tissue stained for BCL2L10 using ab233641 at 1/200 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233641 has not yet been referenced specifically in any publications.