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  1. Link

    products/primary-antibodies/bcl9-antibody-ab37305.pdf

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Epigenetics and Nuclear Signaling Transcription Cancer susceptibility Proto-oncogenes
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Anti-BCL9 antibody (ab37305)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (20)

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Immunocytochemistry/ Immunofluorescence - Anti-BCL9 antibody (ab37305)
  • Western blot - Anti-BCL9 antibody (ab37305)

Key features and details

  • Rabbit polyclonal to BCL9
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-BCL9 antibody
  • Description

    Rabbit polyclonal to BCL9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human BCL9 aa 800-900 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab37304)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Nuclear

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human BCL9 peptide (ab37304)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human BCL9 protein (ab157959)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab37305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use a concentration of 2 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 140 kDa).Can be blocked with Human BCL9 peptide (ab37304).
ICC/IF
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 140 kDa).Can be blocked with Human BCL9 peptide (ab37304).
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Relevance

    BCL9 is involved in signal transduction through the Wnt pathway. It promotes beta-catenin's transcriptional activity. BCL9 is associated with B-cell acute lymphoblastic leukemia. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies (referenced from swissprot and entrez gene).
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 607 Human
    • Entrez Gene: 77578 Mouse
    • Omim: 602597 Human
    • SwissProt: O00512 Human
    • SwissProt: Q1JQ81 Human
    • SwissProt: Q5T489 Human
    • SwissProt: Q9D219 Mouse
    • Alternative names

      • B-cell CLL/lymphoma 9 antibody
      • BCL 9 antibody
      • Legless homolog antibody
      • LGS antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-BCL9 antibody (ab37305)
      Immunocytochemistry/ Immunofluorescence - Anti-BCL9 antibody (ab37305)

      ab37305 stained in HeLa cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37305 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

    • Western blot - Anti-BCL9 antibody (ab37305)
      Western blot - Anti-BCL9 antibody (ab37305)
      All lanes : Anti-BCL9 antibody (ab37305) at 2 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 2 : Jurkat nuclear extract lysate (ab14844)
      Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate
      Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 140 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?
      Additional bands at: 100 kDa, 47 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (20)

    Publishing research using ab37305? Please let us know so that we can cite the reference in this datasheet.

    ab37305 has been referenced in 20 publications.

    • Zhang X  et al. miRNA-122-5p in POI ovarian-derived exosomes promotes granulosa cell apoptosis by regulating BCL9. Cancer Med 11:2414-2426 (2022). PubMed: 35229987
    • Wang Z  et al. Discovery of an Orally Bioavailable Small-Molecule Inhibitor for the β-Catenin/B-Cell Lymphoma 9 Protein-Protein Interaction. J Med Chem 64:12109-12131 (2021). PubMed: 34382808
    • Zimmerli D  et al. TBX3 acts as tissue-specific component of the Wnt/ß-catenin transcriptional complex. Elife 9:N/A (2020). PubMed: 32808927
    • Salazar VS  et al. Reactivation of a developmental Bmp2 signaling center is required for therapeutic control of the murine periosteal niche. Elife 8:N/A (2019). PubMed: 30735122
    • Wang L  et al. Mechanism of Low Expression of miR-30a-5p on Epithelial-Mesenchymal Transition and Metastasis in Ovarian Cancer. DNA Cell Biol 38:341-351 (2019). PubMed: 30839226
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-BCL9 antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (gel 12%)
    Sample
    Human Cell lysate - whole cell (muscle)
    Specification
    muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 30 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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