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Anti-BDNF antibody [3B2] (ab205067)

Submit a review Submit a question References (19)
Western blot - Anti-BDNF antibody [3B2] (ab205067)
  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [3B2] (ab205067)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [3B2] (ab205067)
  • Western blot - Anti-BDNF antibody [3B2] (ab205067)

Key features and details

  • Mouse monoclonal [3B2] to BDNF
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human, Chinese hamster
  • Isotype: IgG1

Overview

  • Product name

    Anti-BDNF antibody [3B2]
    See all BDNF primary antibodies

  • Description

    Mouse monoclonal [3B2] to BDNF

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Chinese hamster

  • Immunogen

    Recombinant full length protein corresponding to Human BDNF (N terminal). (Purified from E.coli).
    Database link: P23560

  • Positive control

    • Brain tissue lysate, proBDNF-expressing U2OS cells; Human cerebral cortex tissue sections from Alzheimer's disease patients.

  • General notes

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab205067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 15 kDa.
ICC/IF
Use a concentration of 0.33 - 20 µg/ml.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 15 kDa.
ICC/IF
Use a concentration of 0.33 - 20 µg/ml.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

  • Tissue specificity

    Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

  • Involvement in disease

    Bulimia nervosa 2
    Congenital central hypoventilation syndrome

  • Sequence similarities

    Belongs to the NGF-beta family.

  • Post-translational
    modifications

    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Abrineurin antibody
    • ANON2 antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • BULN2 antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

Images

  • Western blot - Anti-BDNF antibody [3B2] (ab205067)
    Western blot - Anti-BDNF antibody [3B2] (ab205067)
    All lanes : Anti-BDNF antibody [3B2] (ab205067) at 2.5 µg/ml

    Lane 1 : Human hippocampus with MES
    Lane 2 : Rat hippocampus with MES
    Lane 3 : Mouse hippocampus with MES

    Secondary
    All lanes : Goat anti-Mouse800 Licor at 1/10000 dilution

    Predicted band size: 15 kDa



    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel ran at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for 1 hour before incubation with ab205067 (2.5 microgram /mL) overnight at 4°C.  Antibody binding was detected using infrared labelled goat anti-mouse IgG (green) at a 1:10,000 dilution for 1 hour at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [3B2] (ab205067)
    Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [3B2] (ab205067)

    Immunofluorescent analysis of U2OS cells labeling BDNF with ab205067 at 0.33 µg/ml, followed by Goat anti-mouse AlexaFluor488 secondary antibody. For nuclear staining DAPI was used. A: proBDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [3B2] (ab205067)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [3B2] (ab205067)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebral cortex tissue sections from Alzheimer's disease patients labeling BDNF with ab205067 at 5 µg/ml, followed by peroxidase secondary detection and DAB staining. A: BDNF staining by ab205067; B.Negative staining without primary antibody. Sections were counterstained with toluidine blue.

  • Western blot - Anti-BDNF antibody [3B2] (ab205067)
    Western blot - Anti-BDNF antibody [3B2] (ab205067)
    Anti-BDNF antibody [3B2] (ab205067) at 5 µg/ml + 10 µl of CHO cell culture supernatant containing BDNF at 10 µl

    Secondary
    HRP-conjugated Goat anti-Mouse IgG

    Predicted band size: 15 kDa

References (19)

Publishing research using ab205067? Please let us know so that we can cite the reference in this datasheet.

ab205067 has been referenced in 19 publications.

  • Zhu JP  et al. Baihe Jizihuang Tang Ameliorates Chronic Unpredictable Mild Stress-Induced Depression-Like Behavior: Integrating Network Pharmacology and Brain-Gut Axis Evaluation. Evid Based Complement Alternat Med 2021:5554363 (2021). PubMed: 34475963
  • Ni L  et al. The potential role of the HCN1 ion channel and BDNF-mTOR signaling pathways and synaptic transmission in the alleviation of PTSD. Transl Psychiatry 10:101 (2020). PubMed: 32198387
  • Bae SH  et al. Brain-derived neurotrophic factor mediates macrophage migration inhibitory factor to protect neurons against oxygen-glucose deprivation. Neural Regen Res 15:1483-1489 (2020). PubMed: 31997812
  • Li H  et al. Involvement of the G-Protein-Coupled Receptor 4 in the Increased Expression of RANK/RANKL/OPG System and Neurotrophins by Nucleus Pulposus Cells under the Degenerated Intervertebral Disc-Like Acidic Microenvironment. Biomed Res Int 2020:1328436 (2020). PubMed: 32566653
  • Lorenzana-Martínez G  et al. Effects of Hibiscus sabdariffa calyces on spatial memory and hippocampal expression of BDNF in ovariectomized rats. Nutr Neurosci N/A:1-11 (2020). PubMed: 32787648
View all Publications for this product

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