Anti-BDNF antibody [3B2] (ab205067)
![Western blot - Anti-BDNF antibody [3B2] (ab205067)](https://www.abcam.com/ps/products/205/ab205067/Images/ab205067-256332-anti-bdnf-antibody-3b2-western-blot.jpg "Western blot - Anti-BDNF antibody [3B2] (ab205067)")
Key features and details
- Mouse monoclonal [3B2] to BDNF
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human, Chinese hamster
- Isotype: IgG1
Overview
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Product name
Anti-BDNF antibody [3B2]
See all BDNF primary antibodies -
Description
Mouse monoclonal [3B2] to BDNF -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Chinese hamster -
Immunogen
Recombinant full length protein corresponding to Human BDNF (N terminal). (Purified from E.coli).
Database link: P23560 -
Positive control
- Brain tissue lysate, proBDNF-expressing U2OS cells; Human cerebral cortex tissue sections from Alzheimer's disease patients.
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General notes
For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3B2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 15 kDa.
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| ICC/IF |
Use a concentration of 0.33 - 20 µg/ml.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 15 kDa. |
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ICC/IF
Use a concentration of 0.33 - 20 µg/ml. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. -
Tissue specificity
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. -
Involvement in disease
Bulimia nervosa 2
Congenital central hypoventilation syndrome -
Sequence similarities
Belongs to the NGF-beta family. -
Post-translational
modificationsThe propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG). -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 627 Human
- Entrez Gene: 12064 Mouse
- Entrez Gene: 24225 Rat
- Omim: 113505 Human
- SwissProt: P23560 Human
- SwissProt: P21237 Mouse
- SwissProt: P23363 Rat
- Unigene: 502182 Human
see all -
Alternative names
- Abrineurin antibody
- ANON2 antibody
- BDNF antibody
see all
Images
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Western blot - Anti-BDNF antibody [3B2] (ab205067)All lanes : Anti-BDNF antibody [3B2] (ab205067) at 2.5 µg/ml
Lane 1 : Human hippocampus with MES
Lane 2 : Rat hippocampus with MES
Lane 3 : Mouse hippocampus with MES
Secondary
All lanes : Goat anti-Mouse800 Licor at 1/10000 dilution
Predicted band size: 15 kDaThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel ran at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for 1 hour before incubation with ab205067 (2.5 microgram /mL) overnight at 4°C. Antibody binding was detected using infrared labelled goat anti-mouse IgG (green) at a 1:10,000 dilution for 1 hour at room temperature.
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![Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [3B2] (ab205067)](https://www.abcam.com/ps/products/205/ab205067/Images/ab205067-247725-anti-bdnf-antibody-3b2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [3B2] (ab205067)Immunofluorescent analysis of U2OS cells labeling BDNF with ab205067 at 0.33 µg/ml, followed by Goat anti-mouse AlexaFluor488 secondary antibody. For nuclear staining DAPI was used. A: proBDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [3B2] (ab205067)](https://www.abcam.com/ps/products/205/ab205067/Images/ab205067-247726-anti-bdnf-antibody-3b2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [3B2] (ab205067)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebral cortex tissue sections from Alzheimer's disease patients labeling BDNF with ab205067 at 5 µg/ml, followed by peroxidase secondary detection and DAB staining. A: BDNF staining by ab205067; B.Negative staining without primary antibody. Sections were counterstained with toluidine blue.
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![Western blot - Anti-BDNF antibody [3B2] (ab205067)](https://www.abcam.com/ps/products/205/ab205067/Images/ab205067-247727-anti-bdnf-antibody-3b2-western-blot.jpg)
Western blot - Anti-BDNF antibody [3B2] (ab205067)Anti-BDNF antibody [3B2] (ab205067) at 5 µg/ml + 10 µl of CHO cell culture supernatant containing BDNF at 10 µl
Secondary
HRP-conjugated Goat anti-Mouse IgG
Predicted band size: 15 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab205067 has been referenced in 26 publications.
- Li C et al. Inhibitory effect of miR‑182‑5p on retinal neovascularization by targeting angiogenin and BDNF. Mol Med Rep 25:N/A (2022). PubMed: 34935052
- Yin X et al. Dexmedetomidine Combined with Low-Dose Propofol Declines Learning and Memory Impairment and Neural Cell Injury in Developing Rats. Evid Based Complement Alternat Med 2022:9543459 (2022). PubMed: 35685722
- Mirchandani-Duque M et al. Galanin and Neuropeptide Y Interaction Enhances Proliferation of Granule Precursor Cells and Expression of Neuroprotective Factors in the Rat Hippocampus with Consequent Augmented Spatial Memory. Biomedicines 10:N/A (2022). PubMed: 35740319
- Li Y et al. (2R,6R)-hydroxynorketamine acts through GluA1-induced synaptic plasticity to alleviate PTSD-like effects in rat models. Neurobiol Stress 21:100503 (2022). PubMed: 36532380
- Zhu JP et al. Baihe Jizihuang Tang Ameliorates Chronic Unpredictable Mild Stress-Induced Depression-Like Behavior: Integrating Network Pharmacology and Brain-Gut Axis Evaluation. Evid Based Complement Alternat Med 2021:5554363 (2021). PubMed: 34475963