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    products/primary-antibodies/bdnf-antibody-ab226843.pdf

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Neuroscience Neurology process Growth and Development Neurotrophins
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Anti-BDNF antibody (ab226843)

  • Datasheet
  • SDS
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Western blot - Anti-BDNF antibody (ab226843)
  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (ab226843)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (ab226843)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (ab226843)
  • Western blot - Anti-BDNF antibody (ab226843)

Key features and details

  • Rabbit polyclonal to BDNF
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-BDNF antibody [EPR1292] (ab108319)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-BDNF antibody
    See all BDNF primary antibodies
  • Description

    Rabbit polyclonal to BDNF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Cat, Dog, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide within Human BDNF (internal sequence). The exact sequence is proprietary. Carrier-protein conjugated synthetic peptide.
    Database link: P23560

  • Positive control

    • IHC-P: Adult mouse retina tissue, Rat brain tissue. WB: Mouse and rat brain tissue extract,Transfected (+) 293T whole cell extracts ICC/IF: DIV9 rat E18 primary cortical neuron cells.
  • General notes

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Neurotrophins
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (ab4022)
  • Recombinant Protein

    • Recombinant Human/Murine/Rat BDNF protein (Active) (ab9794)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab226843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.

Dilution is assay dependent.

IHC-P
1/100 - 1/1000.
WB
1/500 - 1/3000. Detects a band of approximately 28 kDa.
Notes
ICC/IF
Use at an assay dependent concentration.

Dilution is assay dependent.

IHC-P
1/100 - 1/1000.
WB
1/500 - 1/3000. Detects a band of approximately 28 kDa.

Target

  • Function

    During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificity

    Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in disease

    Bulimia nervosa 2
    Congenital central hypoventilation syndrome
  • Sequence similarities

    Belongs to the NGF-beta family.
  • Post-translational
    modifications

    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P23560 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 493690 Cat
    • Entrez Gene: 503511 Chimpanzee
    • Entrez Gene: 617701 Cow
    • Entrez Gene: 403461 Dog
    • Entrez Gene: 627 Human
    • Entrez Gene: 12064 Mouse
    • Entrez Gene: 397495 Pig
    • Entrez Gene: 24225 Rat
    • Omim: 113505 Human
    • SwissProt: Q9TST3 Cat
    • SwissProt: Q5IS78 Chimpanzee
    • SwissProt: Q95106 Cow
    • SwissProt: Q7YRB4 Dog
    • SwissProt: P23560 Human
    • SwissProt: P21237 Mouse
    • SwissProt: P14082 Pig
    • SwissProt: P23363 Rat
    • Unigene: 502182 Human
    • Unigene: 1442 Mouse
    • Unigene: 11266 Rat
    see all
  • Alternative names

    • Abrineurin antibody
    • ANON2 antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • BULN2 antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

Images

  • Western blot - Anti-BDNF antibody (ab226843)
    Western blot - Anti-BDNF antibody (ab226843)
    All lanes : Anti-BDNF antibody (ab226843) at 1/500 dilution (4? overnight)

    Lane 1 : Mouse brain
    Lane 2 : Rat brain

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Additional bands at: 17 kDa (possible mature (processed) protein)



    12% SDS-PAGE. 

    Running: 80V, 15min; 140V, 40 min. Transfer: Semi-dry, 18 V, 60 min (NC embrane). Blocking: 5% non-fat milk in TBST, RT, 60 minutes. Washing:  5 ml TBST, 4 x 5 min. ECL exposure.    

     

  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (ab226843)
    Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (ab226843)

    Immunocytochemistry/ Immunofluorescence analysis of DIV9 rat E18 primary cortical neuron cells labeling BDNF with ab226843 at 1:500 (green). Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Cells were counterstained with DAPI (blue) and a Tau antibody diluted at 1/500 (yellow). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (ab226843)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (ab226843)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling BDNF with ab226843 at 1/500 dilution at 4°C overnight. Antigen Retrieval:Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30min. Blocking:1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 minutes. Secondary antibody incubation: ABC HRP Kit (Rabbit IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (ab226843)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (ab226843)

    Immunohistochemical analysis of paraffin-embedded adult mouse retina tissue labeling BDNF with ab226843 at 1/250 dilution (green). Counter nuclear stained with DAPI (blue) and a beta III Tubulin antibody diluted at 1/500 (red). 

  • Western blot - Anti-BDNF antibody (ab226843)
    Western blot - Anti-BDNF antibody (ab226843)
    All lanes : Anti-BDNF antibody (ab226843) at 1/1000 dilution

    Lane 1 : Non-transfected (–) 293T whole cell extracts
    Lane 2 : Transfected (+) 293T whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    15% SDS-PAGE

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (10)

Publishing research using ab226843? Please let us know so that we can cite the reference in this datasheet.

ab226843 has been referenced in 10 publications.

  • Zainullina LF  et al. Dimeric mimetic of BDNF loop 4 promotes survival of serum-deprived cell through TrkB-dependent apoptosis suppression. Sci Rep 11:7781 (2021). PubMed: 33833366
  • Chang M  et al. Genistein acts as antidepressant agent against chronic mild stress-induced depression model of rats through augmentation of brain-derived neurotrophic factor. Brain Behav 11:e2300 (2021). PubMed: 34333865
  • Dong D  et al. The antidepressant effects of l-arginine on chronic mild stress-induced depression by augmenting the expression of brain-derived neurotrophic factor in rats. Brain Res Bull 158:128-134 (2020). PubMed: 32114003
  • Deng W  et al. MicroRNA-130a regulates neurological deficit and angiogenesis in rats with ischaemic stroke by targeting XIAP. J Cell Mol Med 24:10987-11000 (2020). PubMed: 32790238
  • Hu P  et al. Early-life stress alters affective behaviors in adult mice through persistent activation of CRH-BDNF signaling in the oval bed nucleus of the stria terminalis. Transl Psychiatry 10:396 (2020). PubMed: 33177511
  • Chai HH  et al. The chemokine CXCL1 and its receptor CXCR2 contribute to chronic stress-induced depression in mice. FASEB J 33:8853-8864 (2019). PubMed: 31034777
  • Han J  et al. Minor ginsenoside F1 improves memory in APP/PS1 mice. Mol Brain 12:77 (2019). PubMed: 31488185
  • Maruta T  et al. Upregulation of ERK phosphorylation in rat dorsal root ganglion neurons contributes to oxaliplatin-induced chronic neuropathic pain. PLoS One 14:e0225586 (2019). PubMed: 31765435
  • Li S  et al. Therapeutic effect of methylprednisolone combined with high frequency electrotherapy on acute spinal cord injury in rats. Exp Ther Med 18:4682-4688 (2019). PubMed: 31807152
  • Zhang DD  et al. Vildagliptin, a DPP4 inhibitor, alleviates diabetes-associated cognitive deficits by decreasing the levels of apoptosis-related proteins in the rat hippocampus. Exp Ther Med 15:5100-5106 (2018). PubMed: 29805536

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