Anti-BDNF antibody (ab226843)
Key features and details
- Rabbit polyclonal to BDNF
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-BDNF antibody
See all BDNF primary antibodies -
Description
Rabbit polyclonal to BDNF -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Cat, Dog, Pig, Chimpanzee
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Immunogen
Synthetic peptide within Human BDNF (internal sequence). The exact sequence is proprietary. Carrier-protein conjugated synthetic peptide.
Database link: P23560 -
Positive control
- IHC-P: Adult mouse retina tissue, Rat brain tissue. WB: Mouse and rat brain tissue extract,Transfected (+) 293T whole cell extracts ICC/IF: DIV9 rat E18 primary cortical neuron cells.
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General notes
For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
Dilution is assay dependent. |
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| IHC-P |
1/100 - 1/1000.
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| WB |
1/500 - 1/3000. Detects a band of approximately 28 kDa.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. Dilution is assay dependent. |
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IHC-P
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Detects a band of approximately 28 kDa. |
Target
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Function
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. -
Tissue specificity
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. -
Involvement in disease
Bulimia nervosa 2
Congenital central hypoventilation syndrome -
Sequence similarities
Belongs to the NGF-beta family. -
Post-translational
modificationsThe propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG). -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 493690 Cat
- Entrez Gene: 503511 Chimpanzee
- Entrez Gene: 617701 Cow
- Entrez Gene: 403461 Dog
- Entrez Gene: 627 Human
- Entrez Gene: 12064 Mouse
- Entrez Gene: 397495 Pig
- Entrez Gene: 24225 Rat
see all -
Alternative names
- Abrineurin antibody
- ANON2 antibody
- BDNF antibody
see all
Images
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Western blot - Anti-BDNF antibody (ab226843)All lanes : Anti-BDNF antibody (ab226843) at 1/500 dilution (4? overnight)
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Additional bands at: 17 kDa (possible mature (processed) protein)12% SDS-PAGE.
Running: 80V, 15min; 140V, 40 min. Transfer: Semi-dry, 18 V, 60 min (NC embrane). Blocking: 5% non-fat milk in TBST, RT, 60 minutes. Washing: 5 ml TBST, 4 x 5 min. ECL exposure.
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Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (ab226843)Immunocytochemistry/ Immunofluorescence analysis of DIV9 rat E18 primary cortical neuron cells labeling BDNF with ab226843 at 1:500 (green). Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Cells were counterstained with DAPI (blue) and a Tau antibody diluted at 1/500 (yellow).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (ab226843)Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling BDNF with ab226843 at 1/500 dilution at 4°C overnight. Antigen Retrieval:Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30min. Blocking:1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 minutes. Secondary antibody incubation: ABC HRP Kit (Rabbit IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (ab226843)Immunohistochemical analysis of paraffin-embedded adult mouse retina tissue labeling BDNF with ab226843 at 1/250 dilution (green). Counter nuclear stained with DAPI (blue) and a beta III Tubulin antibody diluted at 1/500 (red).
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Western blot - Anti-BDNF antibody (ab226843)All lanes : Anti-BDNF antibody (ab226843) at 1/1000 dilution
Lane 1 : Non-transfected (–) 293T whole cell extracts
Lane 2 : Transfected (+) 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody15% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab226843 has been referenced in 17 publications.
- Ju L et al. BDNF-TrkB signaling-mediated upregulation of Narp is involved in the antidepressant-like effects of (2R,6R)-hydroxynorketamine in a chronic restraint stress mouse model. BMC Psychiatry 22:182 (2022). PubMed: 35291971
- Azam S et al. Dioscin-Mediated Autophagy Alleviates MPP+-Induced Neuronal Degeneration: An In Vitro Parkinson's Disease Model. Molecules 27:N/A (2022). PubMed: 35566180
- Liu HT et al. Baicalein Exerts Therapeutic Effects against Endotoxin-Induced Depression-like Behavior in Mice by Decreasing Inflammatory Cytokines and Increasing Brain-Derived Neurotrophic Factor Levels. Antioxidants (Basel) 11:N/A (2022). PubMed: 35624812
- Yoon EJ et al. Improvement of Cognitive Function in Ovariectomized Rats by Human Neural Stem Cells Overexpressing Choline Acetyltransferase via Secretion of NGF and BDNF. Int J Mol Sci 23:N/A (2022). PubMed: 35628371
- Zainullina LF et al. Dimeric mimetic of BDNF loop 4 promotes survival of serum-deprived cell through TrkB-dependent apoptosis suppression. Sci Rep 11:7781 (2021). PubMed: 33833366