Anti-beta 2 Adrenergic Receptor antibody (ab176490)
Key features and details
- Rabbit polyclonal to beta 2 Adrenergic Receptor
- Suitable for: WB, ELISA
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-beta 2 Adrenergic Receptor antibody
See all beta 2 Adrenergic Receptor primary antibodies -
Description
Rabbit polyclonal to beta 2 Adrenergic Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Horse, Guinea pig, Hamster, Cat, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Mouse beta 2 Adrenergic Receptor (N terminal). The exact sequence is proprietary.
Database link: P18762 -
Positive control
- Rat heart membrane extract, Rat brain stem, HEK293 cells expressing beta 2 Adrenergic Receptor, Lewis rat brain membranes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 47,65 kDa.
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ELISA |
Use at an assay dependent concentration.
(Cellular and membrane) |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 47,65 kDa. |
ELISA
Use at an assay dependent concentration. (Cellular and membrane) |
Target
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Function
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily. -
Post-translational
modificationsPalmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 154 Human
- Entrez Gene: 24176 Rat
- Omim: 109690 Human
- SwissProt: P07550 Human
- SwissProt: P10608 Rat
- Unigene: 2551 Human
- Unigene: 10206 Rat
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Alternative names
- ADRB2 antibody
- ADRB2_HUMAN antibody
- ADRB2R antibody
see all
Images
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Anti-beta 2 Adrenergic Receptor antibody (ab176490) at 2.5 µg/ml + Rat heart membrane extract at 20 µg
Predicted band size: 47,65 kDa
Additional bands at: 65 kDa (possible post-translational modification) -
Cellular ELISA: HEK293 cells expressing beta 2 Adrenergic Receptor were treated with indicated concentrations of agonist (Isoproterenol) and antagonist (CPG12177) and probed with ab176490 (1/1000 of a stock solution of 1µg/μL ) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).
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Membrane ELISA: Lewis rat brain membranes (5 µg/well) were treated with indicated concentrations of agonist (Isoproterenol) and antagonist (CGP12177) and probed with ab176490 (1/1000 of a stock solution of 1µg/μL) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab176490 has been referenced in 3 publications.
- Bhattacharyya R et al. Axonal generation of amyloid-β from palmitoylated APP in mitochondria-associated endoplasmic reticulum membranes. Cell Rep 35:109134 (2021). PubMed: 34010653
- Belay T et al. Modulation of T helper 1 and T helper 2 immune balance in a murine stress model during Chlamydia muridarum genital infection. PLoS One 15:e0226539 (2020). PubMed: 32413046
- Gao V et al. Astrocytic ß2-adrenergic receptors mediate hippocampal long-term memory consolidation. Proc Natl Acad Sci U S A 113:8526-31 (2016). PubMed: 27402767