For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/beta-2-adrenergic-receptor-antibody-epr707n-ab182136.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Signaling Pathway G Protein Signaling GPCR
Share by email
RecombinantRabMAb

Recombinant Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (5) Submit a question References (50)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
  • Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
  • Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
  • Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR707(N)] to beta 2 Adrenergic Receptor
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

You may also be interested in

Primary
Product image
Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)
Protein
Product image
Recombinant Human beta 2 Adrenergic Receptor protein (ab157862)
Primary
Product image
Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)

View more associated products

Overview

  • Product name

    Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]
    See all beta 2 Adrenergic Receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR707(N)] to beta 2 Adrenergic Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver, Human skeletal muscle, Mouse heart, Rat heart, Mouse kidney, Rat kidney, and A431 lysates; IHC-P: Rat stomach, Human stomach and Mouse cerebrum tissues; IHC-Fr: Mouse stomach tissue section.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR707(N)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Myocardial regeneration
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] - BSA and Azide free (ab213651)
    • Alexa Fluor® 647 Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab225294)
    • Alexa Fluor® 488 Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab225295)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human beta 2 Adrenergic Receptor protein (ab157862)
  • Related Products

    • Recombinant Human beta 2 Adrenergic Receptor protein (ab157862)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 46 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For lower background staining, perform heat mediated antigen retrieval with citrate buffer pH 6.

For unpurified use at 1/250.

See IHC antigen retrieval protocols.

IHC-Fr (1)
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 46 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For lower background staining, perform heat mediated antigen retrieval with citrate buffer pH 6.

For unpurified use at 1/250.

See IHC antigen retrieval protocols.

IHC-Fr
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
  • Post-translational
    modifications

    Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
    Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
    Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P07550 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 154 Human
    • Entrez Gene: 11555 Mouse
    • Entrez Gene: 24176 Rat
    • Omim: 109690 Human
    • SwissProt: P07550 Human
    • SwissProt: P18762 Mouse
    • SwissProt: P10608 Rat
    • Unigene: 2551 Human
    • Unigene: 5598 Mouse
    • Unigene: 10206 Rat
    see all
  • Alternative names

    • ADRB2 antibody
    • ADRB2_HUMAN antibody
    • ADRB2R antibody
    • ADRBR antibody
    • Adrenergic beta 2 receptor surface antibody
    • Adrenoceptor beta 2 surface antibody
    • B2AR antibody
    • BAR antibody
    • beta 2 adrenoceptor antibody
    • Beta 2 adrenoreceptor antibody
    • Beta-2 adrenergic receptor antibody
    • Beta-2 adrenoceptor antibody
    • Beta-2 adrenoreceptor antibody
    • BETA2AR antibody
    • Catecholamine receptor antibody
    • OTTHUMP00000160386 antibody
    see all

Images

  • Western blot - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
    Western blot - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
    All lanes : Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136) at 0.1 µg/ml (purified)

    Lane 1 : A431 ( Human epidermoid carcinoma epithelial cell) whole cell lysates
    Lane 2 : Human skeletal muscle lysates
    Lane 3 : Human fetal liver lysates
    Lane 4 : Mouse heart lysates
    Lane 5 : Rat heart lysates
    Lane 6 : Mouse kidney lysates
    Lane 7 : Rat kidney lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

    The 50-90kda bands detected by ab182136 are consistent with many literatures, like PMID: 2836733, PMID: 17570158, PMID: 11701618, PMID: 2545714, PMID: 16708760.

  • Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
    Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)

    IHC image of beta 2 Adrenergic Receptor staining in a section of frozen human prostate carcinoma performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab182136, 1/750 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
    Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)

    Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1/100 (1.3 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1:100 dilution (1.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1:100 dilution (1.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1:100 dilution (1.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
    Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (50)

Publishing research using ab182136? Please let us know so that we can cite the reference in this datasheet.

ab182136 has been referenced in 50 publications.

  • Benítez-Angeles M  et al. Unconventional interactions of the TRPV4 ion channel with beta-adrenergic receptor ligands. Life Sci Alliance 6:N/A (2023). PubMed: 36549871
  • Choi S  et al. Aging Alters the Formation and Functionality of Signaling Microdomains Between L-type Calcium Channels and β2-Adrenergic Receptors in Cardiac Pacemaker Cells. Front Physiol 13:805909 (2022). PubMed: 35514336
  • Ito N  et al. Slc12a8 in the lateral hypothalamus maintains energy metabolism and skeletal muscle functions during aging. Cell Rep 40:111131 (2022). PubMed: 35905718
  • Mai L  et al. Sympathectomy Ameliorates CFA-Induced Mechanical Allodynia via Modulating Phenotype of Macrophages in Sensory Ganglion in Mice. J Inflamm Res 15:6263-6274 (2022). PubMed: 36386581
  • Tao J  et al. Sfrp1 protects against acute myocardial ischemia (AMI) injury in aged mice by inhibiting the Wnt/ß-catenin signaling pathway. J Cardiothorac Surg 16:12 (2021). PubMed: 33468190
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Mouse heart, 293T)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
40 µg
Treatment
293T treated with siRNA
Specification
Mouse heart, 293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 04 2021

Western blot abreview for Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 30 2020

Immunohistochemistry (Frozen sections) abreview for Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (liver)
Permeabilization
Yes - 0.5% Trrton x-100
Specification
liver
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 26 2018

Western blot abreview for Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12 cells)
Gel Running Conditions
Reduced Denaturing (9%)
Loading amount
15 µg
Treatment
400 or 600uM Palmitate for 24hrs
Specification
C2C12 cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 13 2017

Western blot abreview for Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293F)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
293F
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 09 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.