Recombinant Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
![Western blot - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)](https://www.abcam.com/ps/products/182/ab182136/Images/ab182136-316259-anti-beta-2-adrenergic-receptor-antibody-epr707n-western-blot.jpg "Western blot - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR707(N)] to beta 2 Adrenergic Receptor
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]
See all beta 2 Adrenergic Receptor primary antibodies -
Description
Rabbit monoclonal [EPR707(N)] to beta 2 Adrenergic Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human fetal liver, Human skeletal muscle, Mouse heart, Rat heart, Mouse kidney, Rat kidney, and A431 lysates; IHC-P: Rat stomach, Human stomach and Mouse cerebrum tissues; IHC-Fr: Mouse stomach tissue section.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR707(N) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 46 kDa).
|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For lower background staining, perform heat mediated antigen retrieval with citrate buffer pH 6. For unpurified use at 1/250. |
|
| IHC-Fr | (1) |
1/100.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 46 kDa). |
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For lower background staining, perform heat mediated antigen retrieval with citrate buffer pH 6. For unpurified use at 1/250. |
|
IHC-Fr
1/100. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
Target
-
Function
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily. -
Post-translational
modificationsPalmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 154 Human
- Entrez Gene: 11555 Mouse
- Entrez Gene: 24176 Rat
- Omim: 109690 Human
- SwissProt: P07550 Human
- SwissProt: P18762 Mouse
- SwissProt: P10608 Rat
- Unigene: 2551 Human
see all -
Alternative names
- ADRB2 antibody
- ADRB2_HUMAN antibody
- ADRB2R antibody
see all
Images
-
Western blot - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)All lanes : Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136) at 0.1 µg/ml (purified)
Lane 1 : A431 ( Human epidermoid carcinoma epithelial cell) whole cell lysates
Lane 2 : Human skeletal muscle lysates
Lane 3 : Human fetal liver lysates
Lane 4 : Mouse heart lysates
Lane 5 : Rat heart lysates
Lane 6 : Mouse kidney lysates
Lane 7 : Rat kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 46 kDaBlocking and diluting buffer: 5% NFDM/TBST
The 50-90kda bands detected by ab182136 are consistent with many literatures, like PMID: 2836733, PMID: 17570158, PMID: 11701618, PMID: 2545714, PMID: 16708760.
-
![Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)](https://www.abcam.com/ps/products/182/ab182136/Images/ab182136-394211-aanti-beta-2-adrenergic-receptor-epr707n-antibody-immunohistochemistry-frozen-prostate-cancer-human.jpg)
Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)IHC image of beta 2 Adrenergic Receptor staining in a section of frozen human prostate carcinoma performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab182136, 1/750 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
![Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)](https://www.abcam.com/ps/products/182/ab182136/Images/ab182136-10-anti-beta-2-adrenergic-receptor-antibody-epr707-53-immunohistochemistry-frozen-stomach-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1/100 (1.3 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)](https://www.abcam.com/ps/products/182/ab182136/Images/ab182136-316281-anti-beta-2-adrenergic-receptor-antibody-epr707n-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1:100 dilution (1.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)](https://www.abcam.com/ps/products/182/ab182136/Images/ab182136-316280-anti-beta-2-adrenergic-receptor-antibody-epr707n-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1:100 dilution (1.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)](https://www.abcam.com/ps/products/182/ab182136/Images/ab182136-316279-anti-beta-2-adrenergic-receptor-antibody-epr707n-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1:100 dilution (1.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain. -
![Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)](https://www.abcam.com/ps/products/182/ab182136/Images/ab182136-12-benefits-of-recombinant-antibodies.png)
Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (50)
ab182136 has been referenced in 50 publications.
- Benítez-Angeles M et al. Unconventional interactions of the TRPV4 ion channel with beta-adrenergic receptor ligands. Life Sci Alliance 6:N/A (2023). PubMed: 36549871
- Choi S et al. Aging Alters the Formation and Functionality of Signaling Microdomains Between L-type Calcium Channels and β2-Adrenergic Receptors in Cardiac Pacemaker Cells. Front Physiol 13:805909 (2022). PubMed: 35514336
- Ito N et al. Slc12a8 in the lateral hypothalamus maintains energy metabolism and skeletal muscle functions during aging. Cell Rep 40:111131 (2022). PubMed: 35905718
- Mai L et al. Sympathectomy Ameliorates CFA-Induced Mechanical Allodynia via Modulating Phenotype of Macrophages in Sensory Ganglion in Mice. J Inflamm Res 15:6263-6274 (2022). PubMed: 36386581
- Tao J et al. Sfrp1 protects against acute myocardial ischemia (AMI) injury in aged mice by inhibiting the Wnt/ß-catenin signaling pathway. J Cardiothorac Surg 16:12 (2021). PubMed: 33468190